Simultaneous determination of thiamine and riboflavin in enriched cereal based products by high-performance liquid chromatography using selective detection

Mauro, D.; Wetzel, David L.

doi:10.1016/s0021-9673(01)97842-2

Cited by 23 publications

(13 citation statements)

References 11 publications

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“…Post-column derivatization has been proposed for T determinations with conventional columns [17][18][19][20] or more recently with an RP-amide C16 column [21], since it is a method of choice when the stability of fluorescent derivatives is poor. This was evaluated, using a C18 column and a Waters post-column reaction module and external pump.…”

Section: Derivatizationmentioning

confidence: 99%

Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column

Batifoulier¹,

Verny²,

Besson³

et al. 2005

Journal of Chromatography B

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Section: Derivatizationmentioning

confidence: 99%

Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column

Batifoulier¹,

Verny²,

Besson³

et al. 2005

Journal of Chromatography B

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“…11,13 When simultaneous analysis of vitamin B 1 and B 2 , with derivatization of thiamin is attempted, it has the disadvantage that riboflavin may be destroyed during the oxidation step. 14 The method proposed in this paper can be used in routine analysis for royal jelly, having the advantage of being simple, fast and reliable. The quantitative results for riboflavin and pyridoxine are presented in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous high performance liquid chromatographic analysis of vitamins B1, B2 and B6 in royal jelly

Presoto¹,

Rios²,

Almeida-Muradian³

2004

J. Braz. Chem. Soc.

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A geléia real é utilizada como suplemento alimentar, conhecido popularmente como rico em vitaminas B. Os objetivos do presente trabalho foram a determinação simultânea, por cromatografia líquida de alta eficiência, da tiamina (vitamina B 1 ), riboflavina (vitamina B 2 ) e piridoxina (vitamina B 6 ) e a comparação com a indicação da Ingestão Diária Recomendada (DRI). Os valores obtidos variaram de 20 a 171 ng g -1 de riboflavina e 408 a 2 188 ng g -1 de piridoxina nas amostras de geléia real analisadas. A tiamina não foi detectada. Segundo o "Food and Nutrition Board" (2000), as recomendações para estas vitaminas variam de 0,2-1,4 mg para a tiamina; 0,3-1,6 mg para a riboflavina e 0,1-2,0 mg para a piridoxina, dependendo da idade e sexo. Seguindo essas recomendações, a geléia real não pode ser considerada uma boa fonte dessas vitaminas, uma vez que elas aparecem na ordem de nanogramas por grama na geléia real. O método proposto pode ser utilizado em análises de rotina de geléia real tendo as vantagens de ser simples e rápido.Royal jelly is used as a food supplement, popularly known as rich in B vitamins. The present work has two objectives: firstly, to apply simultaneous quantitative determination by High Performance Liquid Chromatography of thiamin (vitamin B 1 ), riboflavin (vitamin B 2 ) and pyridoxine (vitamin B 6 ) and secondly to compare the obtained data with the Dietary Reference Intake (DRI) values. The values obtained showed no thiamin, a range from 20 to 171 ng g -1 of riboflavin and from 408 to 2 188 ng g -1 of pyridoxine in royal jelly. According to the Food and Nutrition Board (2000), the DRI of these vitamins varies from 0.2-1.4 mg for thiamin; 0.3-1.6 mg for riboflavin and 0.1-2.0 mg for pyridoxine, depending on age and sex. According to these recommendations, royal jelly is not a good source of vitamins B 1 , B 2 or B 6 as these vitamins appear only on order of ng g -1 . The proposed method can be used in routine analysis for royal jelly, having the advantage of being simple, fast and reliable.

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“…[9][10][11][12][13] However, the oxidation procedure is relatively time-consuming, and the product of derivatization, thiochrome, has chemical stability issues.…”

mentioning

confidence: 99%

“…10,13,[15][16][17] Ion-pairing reagents improve the chromatographic resolution and peak shape, particularly by reducing peak tailing. With these methods, the mixture of an acetate buffer with 1-octanesulfonate and organic solvents (acetonitrile and methanol) was selected as the mobile phase for the pharmaceutical analysis of thiamine in both United States Pharmacopoeia (USP) 18 and Korean Pharmacopoeia (KP) 19 monographs.…”

mentioning

confidence: 99%

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Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

Suh¹,

Kim²,

Jung³

et al. 2013

Bulletin of the Korean Chemical Society

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A novel green aqueous mobile phase modified with room temperature ionic liquids (RTILs) was employed in the absence of volatile organic solvents or ion-pairing reagents to analyze thiamine, a very polar compound, by reverse phase high performance liquid chromatography (RP-HPLC). Due to its strongly hydrophilic nature, thiamine was eluted near the column dead time (t 0 ) using a mobile phase without adding RTILs or ion-pairing reagents, even if a 100% aqueous mobile phase, which has weak elution power under reverse phase conditions, was used. Thus, 1-ethyl-3-methyl-imidazolium hexafluorophosphate ([EMIM][PF 6 ]), which has the strongest chaotropic effect, was selected as a mobile phase additive to improve retention and avoid baseline disturbances at t 0 . Various mobile phase parameters such as cation moiety, chaotropic anion moiety, pH and concentration of RTILs were optimized to determine thiamine at the proper retention time. Method validation was performed to assess linearity, intra-and inter-day accuracy and precision, recovery and repeatability; all results were found to be satisfactory. The developed method was also compared to the current official United States Pharmacopoeia (USP) and Korean Pharmacopoeia (KP) methods using an organic mobile phase containing an ionpairing reagent by means of evaluating various chromatographic parameters such as the capacity factor, theoretical plate number, peak asymmetry and tailing factor. The results indicated that the proposed method exhibited better efficiency of thiamine analysis than the official methods, and it was successfully applied to quantify thiamine in pharmaceutical preparations.

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Simultaneous determination of thiamine and riboflavin in enriched cereal based products by high-performance liquid chromatography using selective detection

Cited by 23 publications

References 11 publications

Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column

Determination of thiamine and its phosphate esters in rat tissues analyzed as thiochromes on a RP-amide C16 column

Simultaneous high performance liquid chromatographic analysis of vitamins B1, B2 and B6 in royal jelly

Determination of Thiamine in Pharmaceutical Preparations by Reverse Phase Liquid Chromatography Without Use of Organic Solvent

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