Simultaneous determination of thirteen β-blockers and one metabolite by gradient high-performance liquid chromatography with photodiode-array UV detection

Delamoye, Magali; Duverneuil, Charlotte; Paraire, F.; Mazancourt, Philippe de; Alvarez, Jean‐Claude

doi:10.1016/j.forsciint.2003.12.008

Cited by 124 publications

(55 citation statements)

References 21 publications

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“…Atenolol is one of the most commonly prescribed β-blockers, used in antihypertensive, antianginal and antiarrhythmic treatment [19]. After human consumption it is excreted mainly unchanged, leading to its frequent detection in raw wastewater and effluents of the WWTPs [20].…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of atenolol by an enriched nitrifying sludge: Products and pathways

Radjenović

Yuan

et al. 2017

Chemical Engineering Journal

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Biodegradation of β-blocker atenolol was investigated using an enriched nitrifying culture at controlled ammonium concentration and without ammonium addition. Analysis of the kinetics and structural elucidation of biodegradation products showed that atenolol biodegradation was found to be linked to the activity of nitrifying bacteria in the presence of ammonium. Atenolol was degraded cometabolically by ammonia-oxidizing bacteria (AOB), likely due to a broad substrate range of ammonia monooxyenase (AMO). Four products were formed during atenolol biodegradation with ammonia oxidation, including P267 (atenolol acid) and three new products P117 (1-isopropylamino-2-propanol), P167 (1-amino-3-phenoxy-2-propanol), and an unknown product P227 with a nominal molecular mass of 227. In comparison, only P267 and P227 were identified during atenolol biodegradation without ammonia oxidation. Follow-up experiments using atenolol acid as the parent compound indicated the formation of products P117, P167 and P227 in the presence of ammonium. Based on the products identified, a tentative biodegradation pathway of atenolol is suggested, which involves two steps independent of the presence of ammonium: i) microbial amide-bond hydrolysis to carboxyl group and formation of P267 (atenolol acid) and ii) a possible formation of P227 with its unidentified structure and other two cometabolically induced reactions: iii) breakage of ether bond in the alkyl side chain and formation of P117 and iv) a minor pathway through N-dealkylation and loss of acetamide moiety from the aromatic ring, yielding P167. This study provided an important insight regarding the biotransformation pathways under different metabolic conditions.

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Section: Introductionmentioning

confidence: 99%

Biodegradation of atenolol by an enriched nitrifying sludge: Products and pathways

Radjenović

Yuan

et al. 2017

Chemical Engineering Journal

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“…HPLC methods were applied to the simultaneous quantification of selected β-blockers (also: SOT, MET and PRO) in human fluids (plasma, serum, urine). [69][70][71] There is no analytical method for the simultaneous analysis of the 23 selected drugs belonging to four different groups in human urine samples in the scientific literature. It should be emphasized that the simultaneous analysis of several drugs is much more important and effective than the detection of one drug alone in urine samples.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

2009

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We have developed and validated a new and reliable gradient reversed-phase high-performance liquid chromatography (RP-HPLC) method with a diode array detector (DAD) for the simultaneous separation and determination of 23 frequently prescribed selected drugs belonging to different therapeutic groups in human urine samples. For the drugs listed below, this method of analysis for human urine was also successfully applied to determine urine concentrations of these drugs in samples from treated patients: enalapril (ENA), paracetamol (PAR), sotalol (SOT), dipyrone (DIP), vancomycin (VAN), captopril (CAP), fluconazole (FLU), cefazolin (CEF), metoprolol (MET), aspirin (ASP), ticlopidine (TIC), prednisolone (PRE), propranolol (PRO), digoxin (DIG), sildenafil (SIL), furosemide (FUR), dexamethasone (DEX), carvedilol (CAR), ketoprofen (KET), nifedipine (NIF), terbinafine (TER), acenocoumarol (ACE) and spironolactone (SPI). Separation of the analytes was achieved by RP-HPLC-DAD with a mobile phase composed of acetonitrile, methanol and 0.05% trifluoroacetic acid in water using a gradient elution program. Good linear relationships over the investigated concentration ranges were observed with values of r 2 higher than 0.998 for all of the drugs. The intra-day and inter-day precisions of this method were evaluated with RSD values less than 4.26 and 5.42%, respectively. The relative recoveries of the 23 investigated compounds ranged from 93.60 to 106.00% with RSD values less than 4.46%. An expanded uncertainty budget was constructed for all investigated drugs in human urine samples.

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“…A good guide to the work published up to 1991 is presented as the comprehensive monograph in the series Analytical Profiles of Drug Substances [4]. The most recent articles concerning the chromatographic analysis of this drug include high-performance liquid chromatography (HPLC) methods [5][6][7][8][9][10][11] and liquid chromatography/tandem mass spectrometry (LC-MS) [12][13][14]. Regarding CT, it is official in both BP [3] and USP [15].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous determination of celiprolol HCl and chlorthalidone in tablets and biological fluids using high-performance liquid chromatography

Belal¹,

El-Brashy²,

El‐Enany³

et al. 2012

Acta Chromatographica

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Summary.A simple, accurate, and sensitive reversed-phase HPLC method was developed for the simultaneous determination of celiprolol HCl (CE) and chlorthalidone (CT). Good chromatographic separation was achieved using a 250 mm × 4.6 mm i.d., 5 μm particle size Hypurity C8 column. Mobile phase containing a mixture of methanol and 0.04 M phosphate buffer (35:65, v/v) at pH 7.0 was pumped at a flow rate of 1.2 mL min −1 with UV detection at 225 nm. Lisinopril dihydrate (LIS) was used as internal standard. The method showed good linearity in the ranges 0.2-20 and 0.2-10 μg mL −1 with limits of detection 0.06 and 0.04 μg mL −1 and limits of quantification 0.20 and 0.14 μg mL −1 for CE and CT, respectively. The suggested method was successfully applied to the simultaneous analysis of the studied drugs in their synthetic mixture and coformulated tablet. The method was further extended to the determination of CE in biological fluids. The proposed method was also applied to the determination of the studied drugs in presence of some coadministered or related drugs such as atenolol, propranolol, acetazolamide, enalapril, nicardipine, triamterene, and hydrochlorothiazide without any interference.

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Simultaneous determination of thirteen β-blockers and one metabolite by gradient high-performance liquid chromatography with photodiode-array UV detection

Cited by 124 publications

References 21 publications

Biodegradation of atenolol by an enriched nitrifying sludge: Products and pathways

Biodegradation of atenolol by an enriched nitrifying sludge: Products and pathways

Development and Validation of an HPLC Method for the Simultaneous Analysis of 23 Selected Drugs Belonging to Different Therapeutic Groups in Human Urine Samples

Simultaneous determination of celiprolol HCl and chlorthalidone in tablets and biological fluids using high-performance liquid chromatography

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