Simultaneous determination of three Curcuminoids in Curcuma wenyujin Y.H.chen et C.Ling. by liquid chromatography–tandem mass spectrometry combined with pressurized liquid extraction

Shen, Yan; Han, Chao; Chen, Xiangzhun; Hou, Xiandeng; Zhang, Long

doi:10.1016/j.jpba.2013.03.027

Cited by 30 publications

(12 citation statements)

References 15 publications

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“…Curcuminoids are a group of bioactive compounds present in turmeric ( Curcuma longa L.) . In addition, bisdemethoxycurcumin (BDMC) is one of the most abundant curcuminoids, which accounts for 0.011–0.087‰ weight of the dried rhizome . And also, it has attracted increasing interest for various biological activities including antitumor, reduction of bone loss, hypoglycemic, and antioxidant activities .…”

Section: Introductionmentioning

confidence: 99%

Efflux excretion of bisdemethoxycurcumin‐O‐glucuronide in UGT1A1‐overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance‐associated proteins 1 (MRP1) as the glucuronide transporters

et al. 2018

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Bisdemethoxycurcumin (BDMC) was a natural curcuminoid with many bioactivities present in turmeric (Curcuma longa L.). However, the disposition mechanisms of BDMC via uridine 5 0 -diphospho-glucuronosyltransferase (UGT) metabolism still remain unclear. Therefore, we aimed to determine the potential efflux transporters for the excretion of BDMC-O-glucuronide. Herein, chemical inhibition assays (Ko143, MK571, dipyridamole, and leukotriene C4) and biological inhibition experiments including stable knocked-down of breast cancer resistance protein (BCRP), multidrug resistance-associated proteins (MRPs) transporters were both performed in a HeLa cell line stably overexpressing UGT1A1 established previously. The results indicated that Ko143 (5 and 20 μM) caused a marked reduction in excretion rate (18.4-55.6%) and elevation of intracellular BDMC-O-glucuronide (28.8-48.1%), whereas MK-571 (5 and 20 μM) resulted in a significant decrease in excretion rate (6.2-61.6%) and increase of intracellular BDMC-O-glucuronide (maximal 27.1-32.6%). Furthermore, shRNAmediated silencing of BCRP transporter led to a marked reduction in the excretion rate (21.1-36.9%) and an obvious elevation of intracellular glucuronide (24.9%). Similar results were observed when MRP1 was partially silenced. In addition, MRP3 and MRP4 silencing both displayed no obvious changes on the excretion rate and intracellular levels of glucuronide. In conclusion, chemical inhibition and gene silencing results both indicated that generated BDMC-O-glucoside were excreted primarily by the BCRP and MRP1 transporters.

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Section: Introductionmentioning

confidence: 99%

Efflux excretion of bisdemethoxycurcumin‐O‐glucuronide in UGT1A1‐overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance‐associated proteins 1 (MRP1) as the glucuronide transporters

et al. 2018

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“…12 Several methods were reported for the estimation of curcumin in different pharmaceutical and herbal formulations by using UV, 13 HPLC, 14 UPLC, 15 HPTLC, 16 FT-IR 17 and others hyphenated methods. 18,19 However, these techniques are not suitable for analyzing compounds in combinations of polyherbal formulations like Ayurvedic or Chinese medicinal products, since they contain more than one herb. While UV-spectrophotometric methods are more suitable for this objective, studies on dedicated UV-spectrophotometric methods to quantify the curcumin in polyherbal formulations are very limited.…”

Section: Introductionmentioning

confidence: 99%

Development and Validation of UV-Spectrophotometric method for the Estimation of Curcumin in Standardised Polyherbal Formulations

Singh¹,

Avupati²

2017

JYP

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Objective: We aimed to develop and validate a simple UV-spectrophotometric method relates to the estimation of curcumin in standardised polyherbal formulations. Methods: Pure curcumin was received as generous gift sample from Andhra University, India, all solvents and reagents of analytical reagent (AR) grade were procured from local suppliers and readily used for the method development. The method validation parameters were evaluated as per International Conference on Harmonization (ICH) guidelines. Further, this method was applied for the assay of curcumin in marketed product Live-well™, CUMIN (standardised turmeric extract (STE) fortified with standardised black pepper (SBE) extract) capsules using UV-spectrophotometric method. Results and Discussion: It was confirmed from the results of method validation characteristics such as specificity, linearity, range, precision, accuracy, and robustness were keeping within the limits of acceptance criteria defined under ICH guidelines, 76.0560% of curcumin was estimated to be present in each Live-well™, CUMIN capsule containing a minimum 95% of the total curcuminoids (label claimed). Conclusion:In summary, our method was found to be reliable in detecting the unknown percentage of curcumin in Live-well™, CUMIN capsule formulation. Also, the method was proved to be specific, linear, precise, accurate and robust to use for routine analysis of polyherbal formulations containing curcumin as their principal component in the extracts.Key words: UV-spectrophotometric method, Curcumin, ICH Guidelines, Live-well™, CUMIN. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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“…The pigments responsible for yellow color of curcumin are phenolic in nature and are known by the name curcuminoids, which occur naturally in curcumin. Naturally occurring curcuminoids are a mixture of curcumin (77%), demethoxycurcumin, DMC (17%), and bisdemethoxycurcumin, BDMC (3%) [6,7]. Figure 1 highlights the key potential benefits of curcumin with respect to its anticancer, antioxidative, anti-inflammatory, and immunemodulatory activities.…”

Section: Introductionmentioning

confidence: 99%

Structure-Function Elucidation of Antioxidative and Prooxidative Activities of the Polyphenolic Compound Curcumin

Malik

Mukherjee

2014

Chinese Journal of Biology

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Phenolic compounds have been very well known for their antioxidant properties, owing to their unique ability to act as free radical scavengers which, in turn, is an outstanding attribute of their unique biochemical structure. Recent accumulating lines of evidence inculcate sustainable interest and curiosity towards the chemoprotective nature of the natural polyphenolic compound curcumin (diferuloylmethane) against oxidative stress-mediated disorders. Curcumin is naturally found as a constituent of dietary spices called turmeric, extracted from the plant Curcuma longa. However, like every phenolic antioxidant, curcumin possesses a concentration and medium dependent anti-and pro-oxidant behaviour. A detailed study of the structure-function analysis and the understanding of the mode of action of curcumin as well as its chemical analogues is thus essential to understand the selective biochemical consequences of curcumin. Moreover, the presence of transition metal ions, route of administration, and localized tissue are also the vital decisive factors to determine curcumin behaviour. With this viewpoint, this paper sheds lights on the medium dependent prooxidative and antioxidative attributes of curcumin. Further, with respect to emergence of nanocarriers, a brief discussion focusing on the biochemical effect exertion of curcumin chiefly due to targeted and slow release has also been added towards the end.

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Simultaneous determination of three Curcuminoids in Curcuma wenyujin Y.H.chen et C.Ling. by liquid chromatography–tandem mass spectrometry combined with pressurized liquid extraction

Cited by 30 publications

References 15 publications

Efflux excretion of bisdemethoxycurcumin‐O‐glucuronide in UGT1A1‐overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance‐associated proteins 1 (MRP1) as the glucuronide transporters

Efflux excretion of bisdemethoxycurcumin‐O‐glucuronide in UGT1A1‐overexpressing HeLa cells: Identification of breast cancer resistance protein (BCRP) and multidrug resistance‐associated proteins 1 (MRP1) as the glucuronide transporters

Development and Validation of UV-Spectrophotometric method for the Estimation of Curcumin in Standardised Polyherbal Formulations

Structure-Function Elucidation of Antioxidative and Prooxidative Activities of the Polyphenolic Compound Curcumin

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