Simultaneous determination of three diarylheptanoids and an α-tetralone derivative in the green walnut husks (Juglans regia L.) by high-performance liquid chromatography with photodiode array detector

Anti‐proliferative and apoptotic activities of constituents of chloroform extract of Juglans regia leaves

“…Among the isolated compounds, 5,7‐ dihydroxy‐3,4′‐dimethoxyflavone [ 6 ] was isolated from this genus for the first time, whereas 4‐hydroxy‐α – tetralone [ 4 ] and regiolone [ 7 ] have been reported as constituents of J. mandshurica , J. nigra and green husks of J . regia . In addition, compounds 3 and 5 have previously been isolated from J. cathayensis , J. mandshurica and leaves of J. regia .…”

“…In addition, compounds 3 and 5 have previously been isolated from J. cathayensis , J. mandshurica and leaves of J. regia . Lupeol [ 2 ] and 5‐hydroxy‐3,7,4′‐trimethoxyflavone [ 1 ] have also been identified in the Juglandacea genus in previous studies .…”

Determination of a novel diarylheptanoid (Juglanin B) from green walnut husks (Juglans regia L.) in rat plasma by high‐performance liquid chromatography

“…In order to improve the resolution and symmetry of the peak, acetic acid and potassium dihydrogen phosphate were compared as modifi ers. In a previous study, 2% acetic acid was used as a mobile phase modifi er (Liu et al, 2008a), but in this experiment it was found that a more stable baseline could be obtained when using 10 mM potassium dihydrogen phosphate as a modifi er in the mobile phase, which could improve the sensitivity of the analytes. In addition, when the pH value of potassium dihydrogen phosphate solution was adjusted to 3, the mobile phase achieved better separation and symmetry of the peak, because the ionization of phenolic hydroxyl groups in the analytes which were easily ionized in a water system could be restrained under acidic conditions.…”

“…In a previous study by our team, an HPLC method was developed for simultaneous determination of three diarylheptanoids (Juglanin A, Juglanin B, Rhoiptelol) and Regiolone in green walnut husks (Liu et al, 2008a). However, this method was found not to be suitable for plasma samples.…”

“…In order to research the pharmacokinetic behavior of JB, it is necessary to develop a determination method to identify and determine the compound in rat plasma. Until now, there have been only a few reports about determination of these cyclic diarylheptanoids in green walnut husks using HPLC and CE (Liu et al, 2008a;Li et al, 2008). To the best of our knowledge, few analytical methods have been reported for quantitation and determination of the cyclic diarylheptanoid in biological samples by high-performance liquid chromatography (HPLC).…”