Simultaneous determination of three quaternary ammonium muscle relaxants in blood by high performance liquid chromatography-tandem mass spectrometry

Predictive stability, novel HPLC-MS analysis and semi-automatic compounding process for the emergency implementation of a production line of pancuronium in injectable solution.

“…More recently, Zecevic et al , in 2002, published an easy method for the quantification of PC in Pavulon based on high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection ( Zečević et al, 2002 ); it cannot, however, be used to investigate the stability of PC injectable drugs because it does not separate and quantify 3,17-ddPC from the other compounds. It is also of note that several methods have been published to quantify NMBA in biological matrices: fluorometry, HPLC coupled with mass spectrometry (MS), or gas chromatography with nitrogen sensitive detection ( Furuta et al, 1988 , Cirimele et al, 2003 , Huang et al, 2021 ), but these are unable to quantify the four relevant compounds for the assessment of the stability of PC injectable drugs. The stability-indicating quantitative analytical method was used to study the stability of the compounded formulation.…”