Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy-dispersive x-ray fluorescence. 3. Routine analysis.

Maier, E. A.; Dietemann-Molard, A.; Rastegar, F.; Heimburger, Robert F.; Ruch, C.; Maier, A.; Roegel, E; Leroy, M. J. F.

doi:10.1093/clinchem/33.12.2234

Cited by 12 publications

(7 citation statements)

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“…In relation with the analytical techniques used in these studies, most elemental determinations in biofluids have been performed by atomic absorption spectroscopy (AAS) [81,82,83,87,91], particle-induced X-ray emission [95], energy-dispersive X-ray fluorescence [96], inductively coupled plasma atomic emission spectroscopy (ICP-AES) [24,74,79], or inductively coupled plasma mass spectrometry (ICP-MS) [73,76,77,80,84]. The main advantage of using an ICP-MS equipped with a triple quadrupole is the elimination of interferences by operating in either standard single quadrupole (SQ) mode or triple quadrupole (ICP-QQQ-MS).…”

Section: Metal Dyshomeostasis In Lung Cancer: Biofluids and Tissuesmentioning

confidence: 99%

The Metallome of Lung Cancer and its Potential Use as Biomarker

Callejón‐Leblic

Arias-Borrego

Pereira‐Vega

et al. 2019

IJMS

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Carcinogenesis is a very complex process in which metals have been found to be critically involved. In this sense, a disturbed redox status and metal dyshomeostasis take place during the onset and progression of cancer, and it is well-known that trace elements participate in the activation or inhibition of enzymatic reactions and metalloproteins, in which they usually participate as cofactors. Until now, the role of metals in cancer have been studied as an effect, establishing that cancer onset and progression affects the disturbance of the natural chemical form of the essential elements in the metabolism. However, it has also been studied as a cause, giving insights related to the high exposure of metals giving a place to the carcinogenic process. On the other hand, the chemical species of the metal or metallobiomolecule is very important, since it finally affects the biological activity or the toxicological potential of the element and their mobility across different biological compartments. Moreover, the importance of metal homeostasis and metals interactions in biology has also been demonstrated, and the ratios between some elements were found to be different in cancer patients; however, the interplay of elements is rarely reported. This review focuses on the critical role of metals in lung cancer, which is one of the most insidious forms of cancer, with special attention to the analytical approaches and pitfalls to extract metals and their species from tissues and biofluids, determining the ratios of metals, obtaining classification profiles, and finally defining the metallome of lung cancer.

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Section: Metal Dyshomeostasis In Lung Cancer: Biofluids and Tissuesmentioning

confidence: 99%

The Metallome of Lung Cancer and its Potential Use as Biomarker

Callejón‐Leblic

Arias-Borrego

Pereira‐Vega

et al. 2019

IJMS

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“…These elements may be associated with the structure of kaolinite, which is a normal constituent of the soils where tobacco is cultivated and can accumulate in the leaves of the plant. Moreover, it was reported that Fe, Cu, Ni, Rb, Ti and Pb could be found in AM cytoplasm of healthy nonsmokers [20]. The results of a recent study by P .…”

Section: Discussionmentioning

confidence: 85%

Chapter 6: Cell and cell system responses: The delayed respiratory consequences of inhaled radionuclides in population exposed to nuclear catastrophe

Chuchalin¹,

O²,

Chernyaev³

et al. 1995

Stem Cells

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Cytological analysis of bronchoalveolar lavage (BAL) and x-ray energy dispersive spectrometry of intercellular compounds were evaluated in 19 workers ("liquidators") commissioned to clean and contain the nuclear waste after the Chernobyl catastrophe in comparison with ten control subjects. Results showed a significant increase of neutrophils, small monocyte-like alveolar macrophages (AM) and AM with large black cytoplasmic inclusions. In addition, such mineral elements as Zr, Ce, Pr, Sm, Pa, PU and Cs were detected in AM phagosomes in liquidators. These elements were reported as markers of Chernobyl-type nuclear fuel. It is recognized that BAL gives access to particulate matter present in lung parenchyma. Thus, spectrometric analysis of AM inclusions can provide reliable information about the exposure to inhaled radionuclides seven years after the catastrophe.

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“…20 ), PZAA (review in Pleban et al. 19 ), energy dispersive X-ray fluorescence, 30 AAS, 12,13,16,17,22 ETAAS, 10,14 ICP-OES, 10,15 and X-ray fluorescence (with Vitoux et al. 25 ).…”

Section: Resultsmentioning

confidence: 99%

“…12 ); these data indicate an initial disruption to the functioning of the body, 1 thus enabling action to be taken to prevent further deterioration in human health. Since then, research centers have conducted tests on the concentrations of elements, mainly in lymphocytes 1,13–18 and erythrocytes, 1,10,12–17,19–39 although granulocyte 1 and platelets 1,25,40 data have also been obtained.…”

Section: Introductionmentioning

confidence: 99%

Studies of Element Concentration in the Lymphocytes, Erythrocytes, and Plasma of Healthy Human Donors Using Total Reflection X-ray Fluorescence

et al. 2021

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In this article the total reflection X-ray fluorescence (TXRF) analysis was used for determination of element concentrations in three isolated peripheral blood components: lymphocytes, erythrocytes, and plasma, collected from 36 healthy donors (15 men and 21 women) from eastern Poland. The studied blood components were isolated from whole peripheral blood using Histopaque-1077 density gradient centrifugation. In the lymphocytes, the following elements were measured: P, S, Cl, K, Ca, Fe, Zn, Br, Sr and Pb. In the erythrocytes: P, S, Cl, K, Cr, Mn, Fe, Cu, Zn, Se, Br, Rb and Pb, while in the plasma samples: P, S, Cl, K, Ti, V, Cr, Fe, Co, Ni, Cu, Zn, Br, Se, Sr and Pb. The descriptive statistic parameters of concentration such as: mean value, standard deviation, median, 1st and 3rd quartiles, 10th and 90th percentiles were calculated for all samples of the studied blood components, as well as separately for male and female groups. The measured element concentrations and calculated parameters can be used as the reference values. Element concentration distributions for male and female groups were statistically compared using the non-parametric Mann-Whitney test and statistical significance differences (Î± = 0.05) were found for: P (in lymphocytes), Se and Rb (in erythrocytes) and V (in plasma). The multi-group statistical comparison of element concentration distribution for different blood components was also done (Mann-Whitney and Kruskal-Wallis tests). The statistical test show that, the concentration levels are usually different, except in the following cases: Zn, Fe (lymphocytes and plasma, Fe only for female group), Cr (erythrocytes and plasma). The obtained concentration ranges were compared with literature-based data available for element concentration in lymphocytes, erythrocytes, and plasma. In this work, the application of the TXRF method allowed additionally for the simultaneous determination of the concentrations of such elements as: P, S, Cl, Br, Sr and Pb in lymphocytes, S, Cl and Br in erythrocytes, and P, S, Cl, K, Ti, Br and Pb in plasma. From analytical point of view, the article describes in details the measurement procedure including the isolation of blood components and samples preparation for TXRF measurements, and later the detection limit of the method is discussed.

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Simultaneous determination of trace elements in lavage fluids from human bronchial alveoli by energy-dispersive x-ray fluorescence. 3. Routine analysis.

Cited by 12 publications

References 0 publications

The Metallome of Lung Cancer and its Potential Use as Biomarker

The Metallome of Lung Cancer and its Potential Use as Biomarker

Chapter 6: Cell and cell system responses: The delayed respiratory consequences of inhaled radionuclides in population exposed to nuclear catastrophe

Studies of Element Concentration in the Lymphocytes, Erythrocytes, and Plasma of Healthy Human Donors Using Total Reflection X-ray Fluorescence

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