Simultaneous determination of tramadol and paracetamol in human plasma using LC-MS/MS and application in bioequivalence study of ­fixed-dose combination

Loh, Gabriel Onn Kit; Wong, Emily Yii Ling; Goh, Chen Zhu; Tan, Yvonne Tze Fung; Lee, Yi Lin; Pang, Lai Hui; Shahridzo, Siti Halimah; Damenthi, Nair; Hermansyah, Andi; Long, Chiau Ming; Peh, Kok Khiang

doi:10.1080/07853890.2023.2270502

Cited by 2 publications

(1 citation statement)

References 63 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Most often, these are preparations available on the pharmaceutical market. Some of them were conducted in combination with HPLC and mass spectrometry [17][18][19][20][21]. Many analysts, however, tend to use a chromatographic method that is simpler, taking into account, for example, sample preparation.…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography

Bober-Majnusz,

Pyka-Pająk

2024

Processes

View full text Add to dashboard Cite

TLC (thin-layer chromatography) and HPTLC (high-performance thin-layer chromatography) in normal (NP) and reversed (RP) phase systems were combined with densitometry to analyze caffeine, propyphenazone, and paracetamol. This work aims to check whether comparable limit of detection (LOD) values can be obtained on TLC and HPTLC plates. Analyses were performed on five (NP) or four (RP) different stationary phases (chromatographic plates), testing, in both cases, three mobile phases. It is shown that by using both TLC and HPTLC plates, it is possible to develop chromatographic conditions that enable the detection of compounds analyzed in amounts ranging from a dozen to several dozen µg/spot. In the RP system, lower LOD values for all tested compounds were obtained using TLC than HPTLC. However, performing analyses in the NP, similar (of the same order) LOD values were obtained for caffeine, propyphenazone, and paracetamol when using both TLC and HPTLC plates. For example, during the NP-HPTLC analysis using silica gel 60F254 plates (#1.05548) and mobile phase B (n-hexane—acetone—ammonia, 25:25:0.5, v/v/v), LOD values for caffeine, propyphenazone, and paracetamol were 0.010, 0.046, and 0.030 μg/spot, respectively. During NP-TLC analysis using silica gel 60F254 (#1.05554 plates) and the mobile phase C (chloroform—toluene—ethyl acetate—methanol—80% acetic acid, 18:18:7.5:6:0.3, v/v), the values of LOD were 0.054, 0.029, and 0.016 μg/spot, respectively. During RP-TLC analysis using TLC RP-18F254 plates (#1.05559) and mobile phase F (methanol-water, 40:10, v/v), the LOD values were 0.019, 0.024, and 0.053 μg/spot, respectively. Therefore, for economical reasons, TLC plates should be recommended for analyses of caffeine, propyphenazone, and paracetamol, which are several times cheaper than HPTLC plates.

show abstract

Section: Introductionmentioning

confidence: 99%

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography

Bober-Majnusz,

Pyka-Pająk

2024

Processes

View full text Add to dashboard Cite

show abstract

From surface to core: Comprehensive ToF-SIMS insights into pharmaceutical tablet analysis

Finšgar

2024

Microchemical Journal

View full text Add to dashboard Cite

Simultaneous determination of tramadol and paracetamol in human plasma using LC-MS/MS and application in bioequivalence study of fixed-dose combination

Cited by 2 publications

References 63 publications

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography

From surface to core: Comprehensive ToF-SIMS insights into pharmaceutical tablet analysis

Contact Info

Product

Resources

About

Simultaneous determination of tramadol and paracetamol in human plasma using LC-MS/MS and application in bioequivalence study of ­fixed-dose combination

Cited by 2 publications

References 63 publications

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography

Comparison of the Limit of Detection of Paracetamol, Propyphenazone, and Caffeine Analyzed Using Thin-Layer Chromatography and High-Performance Thin-Layer Chromatography

From surface to core: Comprehensive ToF-SIMS insights into pharmaceutical tablet analysis

Contact Info

Product

Resources

About

Simultaneous determination of tramadol and paracetamol in human plasma using LC-MS/MS and application in bioequivalence study of fixed-dose combination