Simultaneous determination of triamcinolone acetonide palmitate and triamcinolone acetonide in beagle dog plasma by UPLC-MS/MS and its application to a long-term pharmacokinetic study of triamcinolone acetonide palmitate lipid emulsion injection

Liu, Hui; Yang, Mingjing; Wu, Panpan; Guan, Jiao; Men, Lei; Lin, Hongli; Tang, Xing; Zhao, Yunli

doi:10.1016/j.jpba.2014.11.028

Cited by 12 publications

(10 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The parameters of the fragmentor and collision energies were optimized in order to obtain the richest relative abundance of precursor and product ions. The optimization of the parameters was sensitive and stable, and could be applied to pharmacokinetic investigation of peimine and peiminine [ 30 ]. Theophylline was used as the internal standard (IS) as it exhibited a stable response, effective separation, and made this analytical method more accurate.…”

Section: Resultsmentioning

confidence: 99%

“…There are several reports on assaying peimine and peiminine in Bulbus Fritillaria [ 18 , 19 , 23 , 31 ], but no analytical method was developed for the simultaneous determination of the given two alkaloids in FU and FT powder, Most studies mainly focused on the determination of several other alkaloids present in high contents. Liu et al developed a method to determine the two alkaloids simultaneously from alkaloid extracts of Bulbus Fritillaria in rat plasma [ 30 ]. In this method, the LLOQs were 1.0 ng/mL for both peimine and peiminine and the run time was 11.2 min.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder

et al. 2018

View full text Add to dashboard Cite

A simple and high sensitive ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was developed and validated for the simultaneous determination of peimine and peiminine in beagle dog plasma after the oral administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq powder. Chromatographic separation was achieved on an ACQUIT UPLC® BEH C18 column (1.7 μm, 2.1 × 100 mm) in a gradient elution way with a mobile phase consisting of acetonitrile and water containing 0.1% formic acid at a flow rate of 0.4 mL/min. The plasma samples were prepared by a liquid–liquid extraction (LLE) method with ethyl acetate. The analytes were detected with a triple quadrupole tandem mass spectrometry (MS) in multiple reaction monitoring (MRM) mode and a positive ion electrospray ionization (ESI) of the transitions at m/z 432.4→414.4 for peimine and m/z 430.3→412.3 for peiminine. The method was linear for two analytes over the investigated range with all determined correlation coefficients exceeding 0.9900. The lower limit of quantification (LLOQ) was 0.988 ng/mL for peimine and 0.980 ng/mL for peiminine. The mean extraction recoveries of peimine and peiminine at three quality control samples (QC) levels were ranged from 82.56 to 88.71%, and matrix effects ranged from 92.06 to 101.2%. The intra-day and inter-day precision and accuracy were within the acceptable limits at LLOQ and QC levels. The method was effectively and successfully applied to the pharmacokinetics of peimine and peiminine after oral administration of powder to beagle dogs. The obtained results may be help to guide the clinical application of Fritillaria ussuriensis Maxim and Fritillaria thunbergii Miq.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Therefore, sequentially to further examine the mechanisms of action of the enantiomers or diastereomers, it is necessary to understand which compounds are present at the site of action. Therefore, there is an increased demand for sensitive analytical methods to quantify and evaluate the chirality of metabolites present in biological fluids [96]. UHPLC is an incredible tool for the present rehearsing chromatographer, as it can essentially expand the output of a chromatographic separation.…”

Section: Uhplc In Separation Of Isomersmentioning

confidence: 99%

Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

et al. 2019

View full text Add to dashboard Cite

Background: UHPLC-MS/MS is connected in various research facilities for the qualitative and quantitative investigation of a pharmaceutical substance, pharmaceutical items, and biological specimen. Main body: The commence review article is an endeavor to offer pervasive awareness around assorted aspects and details about the UHPLC-MS/MS and related techniques with the aim on practice to an estimation of medicinal active agents in the last 10 years. The article also focused on isolation, separation, and characterization of present impurity in drug and biological samples. Conclusion: Review article compiles a general overview of medicinally important drugs and their analysis with UHPLC-MS/MS. It gives fundamental thought regarding applications of UHPLC-MS/MS for the study on safety limit. The summary of developed UHPLC-MS/MS methods gives a contribution to the future trend and limitations in this area of research.

show abstract

“…A large number of methods have been reported for the determination of TCA in complex matrices, mainly based on LC with different detection modes . For example, Fernandez‐Cunha et al.…”

Section: Introductionmentioning

confidence: 99%

“… applied a HPLC–UV method to determine TCA in the aqueous humor of rabbit's eyes, whereas Liu et al. and Leporati et al. used the HPLC–MS method to quantify TCA in plasma and urine samples, respectively.…”

Section: Introductionmentioning

confidence: 99%

Development of an ultra high performance liquid chromatography method for determining triamcinolone acetonide in hydrogels using the design of experiments/design space strategy in combination with process capability index

et al. 2016

View full text Add to dashboard Cite

An ultra high performance liquid chromatography method was developed and validated for the quantitation of triamcinolone acetonide in an injectable ophthalmic hydrogel to determine the contribution of analytical method error in the content uniformity measurement. During the development phase, the design of experiments/design space strategy was used. For this, the free R-program was used as a commercial software alternative, a fast efficient tool for data analysis. The process capability index was used to find the permitted level of variation for each factor and to define the design space. All these aspects were analyzed and discussed under different experimental conditions by the Monte Carlo simulation method. Second, a pre-study validation procedure was performed in accordance with the International Conference on Harmonization guidelines. The validated method was applied for the determination of uniformity of dosage units and the reasons for variability (inhomogeneity and the analytical method error) were analyzed based on the overall uncertainty.

show abstract

Simultaneous determination of triamcinolone acetonide palmitate and triamcinolone acetonide in beagle dog plasma by UPLC-MS/MS and its application to a long-term pharmacokinetic study of triamcinolone acetonide palmitate lipid emulsion injection

Cited by 12 publications

References 22 publications

Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder

Simultaneous Determination and Pharmacokinetics of Peimine and Peiminine in Beagle Dog Plasma by UPLC-MS/MS after the Oral Administration of Fritillariae ussuriensis Maxim and Fritillariae thunbergii Miq Powder

Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

Development of an ultra high performance liquid chromatography method for determining triamcinolone acetonide in hydrogels using the design of experiments/design space strategy in combination with process capability index

Contact Info

Product

Resources

About