Simultaneous determination of triclabendazole and its metabolites in bovine and goat tissues by liquid chromatography–tandem mass spectrometry

Development and validation of a rapid selective high-throughput LC-MS/MS method for the determination of triclabendazole sulfoxide concentrations in sheep plasma and its use in bioequivalence studies

“…The determination of drug concentrations in plasma or other biological samples through liquid chromatography coupled with mass spectrometry (LC-MS/MS) methods is now considered a technological prerequisite for highly specific and sensitive analysis. Determination of triclabendazole metabolites from different types of biological matrices in animals has been described in several studies using different analytical methods [10][11][12][13][14][15][16]. Several methods reported in literature use high performance liquid chromatography (HPLC) to determine triclabendazole and its metabolites in the plasma of different species [10,13,14].…”

“…Several methods reported in literature use high performance liquid chromatography (HPLC) to determine triclabendazole and its metabolites in the plasma of different species [10,13,14]. In recent years, several LC-MS/MS methods have been described in literature for the determination of triclabendazole and its metabolites from bovine milk [15,16], and fat, muscle, liver and kidney tissues of cattle and goat [11,12]. No methods have yet been described in literature for the determination of triclabendazole or its metabolites from ovine plasma using a validated LC-MS method.…”

“…The total runtime for each sample was more than 40 min and for sample purification solid phase extraction was used in this methodology. A research team lead by Chengyuan Cai developed LC-MS methodologies used to determine triclabendazole and its metabolites from different types of bovine and goat matrices, such as muscle tissues and internal organs (kidney, liver) [11], as well as fat tissue [12]. Muscle, kidney and liver samples were purified using extraction of interfering compounds with hexane.…”

“…Muscle, kidney and liver samples were purified using extraction of interfering compounds with hexane. Analytical separation was performed on an XTerra MS C18 HPLC column with mobile phase consisting of acetronitrile and aqueous formic acid solution in gradient elution, while detection was carried out after positive electrospray ionization, having a total runtime of 20 min for each sample [11]. For analysis of triclabendazole and its metabolites from fat tissues Cai and his research team optimized the previous method, simplifying the sample purification of samples.…”

Application of liquid chromatographic method with fluorescence detection for the determination of triclabendazole in tablets and biological fluids

“…A literature survey revealed many methods for the analysis of TCB, none of them was an high‐performance liquid chromatography (HPLC) method with fluorescence detection. These methods include spectrophotometric methods , HPLC methods with ultraviolet detection , and mass spectrometry . This paper presents a simple, sensitive and accurate method for the determination of TCB in pure and in laboratory prepared tablets, and in biological fluids.…”