Simultaneous determination of tryptophan and 8 metabolites in human plasma by liquid chromatography/tandem mass spectrometry

Boulet, Lysiane; Faure, Patrice; Flore, Patrice; Montérémal, Julien; Ducros, Véronique

doi:10.1016/j.jchromb.2017.04.010

Cited by 60 publications

(40 citation statements)

References 39 publications

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“…Concentrations of the metabolites in human plasma samples from six healthy volunteers (three male and three female) are described in Table 2. The results were consistent with those of previous reports [23,24] [(3HK was 14-54 nmol/L, KYN was 1.2-3.4 μmol/L, 5HT was <4-180 nmol/L, 5HTP was <2.3-8 nmol/L, 3HA was 5-51 nmol/L, Trp was 45-88 μmol/L, XA was 12-33 nmol/L, and KA was 21-66 nmol/L) and (5HIAA was 13.0 ng/mL, IAA was 291.5 ng/mL, KA was 7.9 ng/mL, PA was 5.4 ng/mL, QA was 69.0 ng/mL]. To the best of our knowledge, there is no reported data of ILA in human plasma.…”

Section: Human Plasma Analysissupporting

confidence: 94%

“…Various methods of analyzing Trp metabolites have been reported [17][18][19][20][21][22][23][24]. In our previous studies, we have developed analytical methods to quantify amino acids and their metabolites in human plasma using pre-column derivatization with a 3-aminopyridyl-N-hydroxy succinimidyl carbamate (APDS) reagent and liquid chromatography tandem mass spectrometry (LC-MS/MS) [25].…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Analysis of Tryptophan and Its Metabolites in Human Plasma Using Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

et al. 2019

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A simplified method for analyzing tryptophan (Trp) and its metabolites in human plasma was developed using liquid chromatography-electrospray ionization tandem mass spectrometry. Trp and its metabolites have various chemical properties but have no common functional group for derivatization. Using a reversed-phase pentafluorophenyl (PFP) column for liquid chromatography separation, Trp and its 15 metabolites (3-hydroxyanthranilic acid, 3-hydroxy-kynurenine, 3-indoleacetic acid, 5-hydroxyindole-3-acetic acid, 5-hydroxy-L-tryptophane, anthranilic acid, indole-3-lactic acid, kynurenine, kynurenic acid, melatonin, nicotinic acid, picolinic acid, quinolinic acid, serotonin, and xanthurenic acid were successfully separated within 15 min without derivatization. Neopterin which is known as a biomarker for inflammation and is often evaluated with Trp metabolites in several reports could also be simultaneously analyzed. With this method, Trp and its metabolites were detected with good sensitivity and selectivity without derivatization and solid-phase extraction. The method was validated in this study, showing that the relative standard deviation of 14 Trp metabolites was <15%, and accuracy was within 100% ± 20%, with the exception of nicotinic acid. The quantification range was optimized to 0.0150-100 µM covering the concentration of Trp metabolites in human plasma. Overall, 12 Trp metabolites in healthy human plasma were quantified with good precision and accuracy.

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Section: Human Plasma Analysissupporting

confidence: 94%

Section: Introductionmentioning

confidence: 99%

Simultaneous Analysis of Tryptophan and Its Metabolites in Human Plasma Using Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

et al. 2019

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“…However, all of these clinical diagnostic guidelines do not, or only superficially, cover MS-based metabolomics and lipidomics. Furthermore, only a few MS-based methods have been published that were validated according to such guidelines ( 42 – 47 ). Recently, the Clinical Laboratory Standards Institute (CLSI) issued the CLSI C62-A guideline on MS in the clinical laboratory ( 48 , 49 ).…”

Section: Pre-analyticsmentioning

confidence: 99%

MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines

Burla

Arita

et al. 2018

Journal of Lipid Research

261

227

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Human blood is a self-regenerating lipid-rich biological fluid that is routinely collected in hospital settings. The inventory of lipid molecules found in blood plasma (plasma lipidome) offers insights into individual metabolism and physiology in health and disease. Disturbances in the plasma lipidome also occur in conditions that are not directly linked to lipid metabolism; therefore, plasma lipidomics based on MS is an emerging tool in an array of clinical diagnostics and disease management. However, challenges exist in the translation of such lipidomic data to clinical applications. These relate to the reproducibility, accuracy, and precision of lipid quantitation, study design, sample handling, and data sharing. This position paper emerged from a workshop that initiated a community-led process to elaborate and define a set of generally accepted guidelines for quantitative MS-based lipidomics of blood plasma or serum, with harmonization of data acquired on different instrumentation platforms across independent laboratories as an ultimate goal. We hope that other fields may benefit from and follow such a precedent.

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“… did not describe their derivatization conditions. Despite the high sensitivity of fluorescence detection, identification of Trp in human plasma remains challenging and some authors failed to do it , while some others argued that Trp should be quantitated in plasma at a concentration around 54 µM . Boulat et al.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoresis/visible‐LED induced fluorescence of tryptophan: What's new?

Perquis

Ong-Meang

et al. 2019

Electrophoresis

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Tryptophane (Trp) labelled by 3‐(4‐carboxybenzoyl)‐2‐quinolinecarboxaldehyde (CBQCA) is very difficult to identify using CE and fluorescence detection (480 nm). Why in this article some mass spectrometry experiments show that Trp is really labelled by CBQCA as Leucine (Leu)? If the maximum of UV absorption (λmax) is the same between Leu‐CBQCA and Trp‐CBQCA, the molar extinction coefficient is around 2 fold higher for Trp‐CBQCA. The fluorescence of the Leu‐CBQCA derivative is 50 times more important than for Trp‐CBQCA. The addition of 7.5 mM of β‐cyclodextrin (β‐CD) was found to be a good mean to improve 2.1 fold the sensitivity of the Trp‐CBQCA fluorescence. Using a buffer containing SDS and β‐CD in CE, a LOD of 0.7 µM of L‐Trp can be reached and the ratio of the intensities between Leu, Isoleucine, Valine, Trp is 100, 21, 15, 1. Negative ESI/MS and MS/MS of the labeled amino acids show that a loss of the carboxylate function takes place. In the presence of two enantiomers of Trp‐CBQCA, we have shown that this decarboxylation is not due to the derivatization process in the solution but rather occurs in the source of the mass spectrometer.

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Simultaneous determination of tryptophan and 8 metabolites in human plasma by liquid chromatography/tandem mass spectrometry

Cited by 60 publications

References 39 publications

Simultaneous Analysis of Tryptophan and Its Metabolites in Human Plasma Using Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

Simultaneous Analysis of Tryptophan and Its Metabolites in Human Plasma Using Liquid Chromatography–Electrospray Ionization Tandem Mass Spectrometry

MS-based lipidomics of human blood plasma: a community-initiated position paper to develop accepted guidelines

Capillary electrophoresis/visible‐LED induced fluorescence of tryptophan: What's new?

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