Simultaneous determination of twelve inorganic and organic arsenic compounds by liquid chromatography–ultraviolet irradiation–hydride generation atomic fluorescence spectrometry

Simon, Stéphane; Tran, Lan Huong; Pannier, Florence; Potin‐Gautier, Martine

doi:10.1016/j.chroma.2003.09.068

Cited by 64 publications

(40 citation statements)

References 35 publications

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“…8 Better sensitivity has been achieved with the use of HG-atomic fluorescence spectrometry (HG-AFS). [9][10][11] Nevertheless, ICP-MS [12][13][14][15][16][17][18][19] is widely used for arsenic speciation analysis since it offers extremely high sensitivity, large dynamic range and easy coupling to HPLC. Especially, electrospray tandem mass spectrometry (ES-MS/MS) 4,20-23 was used for structural verification of arsenosugar compounds.…”

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confidence: 99%

Determination of Arsenic Species in Marine Samples by HPLC-ICP-MS

2006

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IntroductionAs far we have developed the very high sensitive methods for determining trace and ultratrace elements by combination between flow injection analysis (FIA) with on-line column preconcentrating techniques and inductively coupled plasma mass spectrometry (ICP-MS), such as trace metals (Cr(III), Al, V, Mn, Co, Ni, Cu, Zn, Mo, Cd, Pb and U) in seawater 1-3 and rare earth elements (Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu) in seawater. [4][5][6] Therefore, since an ICP-MS instrument is a high sensitive detector, a hyphenated method by combination between high performance liquid chromatography (HPLC) and ICP-MS, is promising to be sensitive.Speciation of arsenic has acquired significant attention over the past 20 years in both mechanistic and exposure assessment research, because of toxicity of arsenic. While inorganic arsenic species As V and As III have been classified as carcinogenic, the methylated forms, MMA, and DMA have recently been identified as cancer promoters. It is believed that arsenobetaine and arsenocholine, which are highly methyl substituted, are considered to be non toxic. Depending on the kind of organism, different kind and concentrations of arsenic compounds were observed.In fish and crustaceans, arsenobetaine is a dominant species along with trace of MMA, DMA, TeMAs and TMAO. [1][2][3][4][5][6] In marine organism, more than 17 different inorganic and organic species have been reported. 7 In marine algae, arsenosurgars compounds (derivatives of dimethylarsinoylribosides and trimethylarsonioribosides) are most abundant. [2][3][4][5][6][7] The analytical speciation of arsenic has been achieved with the use of coupled techniques, which combine a separation process (chromatography) with suitable detection. Hydride generation atomic absorption spectrometry (HG-AAS) has been used for arsenic speciation in which online thermal or microwave oxidation was employed for converting many organoarsenicals into hydride forming one. 8 Better sensitivity has been achieved with the use of HG-atomic fluorescence spectrometry (HG-AFS). [9][10][11] Nevertheless, ICP-MS [12][13][14][15][16][17][18][19] is widely used for arsenic speciation analysis since it offers extremely high sensitivity, large dynamic range and easy coupling to HPLC. Especially, electrospray tandem mass spectrometry (ES-MS/MS) 4,20-23 was used for structural verification of arsenosugar compounds.Arsenicals have widely varying ionic characteristics, which are pH dependent. The anionic species As III , As V , DMA and MMA, can usually be resolved using anion exchange HPLC. Arsenobetaine (pKa 2.2) may be cationic or zwitter ionic. Cation exchange can be used to separate the cationic species arsenobetaine, arsenocholine, TeMAs and TMAO. 13Alternatively, ion pairing reversed phase HPLC can be used to resolve inorganic and organoarsenicals in single chromatographic run with help of ion pairing agents. Tetrabutyl ammonium hydroxide is common ion pairing agent used for separating anionic species and pentanesulfonate or hex...

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Determination of Arsenic Species in Marine Samples by HPLC-ICP-MS

2006

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“…Most of them are based on chromatographic separation techniques such as High Performance Liquid Chromatography (HPLC) (Gailer and Irgolic, 1996;Teräsahde et al, 1996;Le and Ma, 1997;Dagnac et al, 1999;Kohlmeyer et al, 2002) or Capillary Zone Electrophoresis (Van Holderbeke et al, 1999), coupled with a selective and sensitive detector such as Atomic Absorption Spectrometry (AAS) (Gailer and Irgolic, 1996) or Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) (Teräsahde et al, 1996;Kohlmeyer et al, 2002). Post column derivatization of arsenic species with formation of the volatile arsine hydrides, has been coupled with AAS (Le and Ma, 1997), Atomic Fluorescence Spectrometry (Simon et al, 2004) or ICP-MS (Dagnac et al, 1999), but cannot be used for quantifying AsBet without prior chemical conversion (Slejkovec et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Speciation of five arsenic species (arsenite, arsenate, MMAAV, DMAAV and AsBet) in different kind of water by HPLC-ICP-MS

et al. 2007

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A method using Ion Chromatography hyphenated to an Inductively Coupled Plasma-Mass Spectrometer has been developed to accurately determine arsenite (As III ), arsenate (As V ), mono-methylarsonic acid (MMAA V ), dimethylarsinic acid (DMAA V ) and arsenobetaine (AsBet) in different water matrices. The developed method showed a high sensitivity with detection limits for each arsenic species close to 0.4 pg injected. Arsenite and arsenate were the major species found in surface and well waters, but AsBet and DMAA V were found in some surface waters, which has never been reported before, while in some natural mineral waters located in volcanic region, the arsenic content exceeded the maximal admissible arsenic content by European legislation standards and the predominant form was As V .

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“…Up to twelve arsenic species have been determined in a single run with an anion-exchange column. 42 Detection limits in the mg L À1 level or lower are easily achieved with HPLC-HG-AFS. As an example, detection limits of 0.2, 0.1, 0.2, 0.2 and 0.3 mg L À1 have been reported for each of the following species: As(III), As(V), DMA, MMA and AsB (the later with a postcolumn UV photo-oxidation step).…”

Section: Speciation With Chromatographic Separationmentioning

confidence: 99%

Atomic Fluorescence Spectrometry: a suitable detection technique in speciation studies for arsenic, selenium, antimony and mercury

Sánchez-Rodas

Corns

Chen

et al. 2010

J. Anal. At. Spectrom.

250

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Atomic Fluorescence Spectrometry (AFS) is an ideal detection technique for speciation studies concerning hydride forming elements (mainly As, Se and Sb) and Hg. The analytical features of AFS, such as detection limits below the mg L À1 and the wide linear calibration range, up to the mg L À1, allow its application to a great variety of environmental, biological and food samples. AFS represents a suitable alternative to other atomic spectrometers commonly employed in speciation studies such as Atomic Absorption Spectrometry (AAS) and Inductively Coupled Plasma-Mass Spectrometry (ICP-MS). The instrumentation used for AFS and the design of the vapour generation and optical layouts required to sustain the full benefits of the AFS approach are also described. The present review explains and comments on the instrumental couplings of chromatographic (HPLC and GC) and nonchromatographic separations (CE) with AFS detection, with online hydride generation for the speciation of inorganic and organic compounds of As, Se and Sb, and cold vapour for Hg. Other optional intermediate steps are online photo-oxidation (UV), pyrolysis or Microwave Assisted Digestion (MAD) for non-directly reducible compounds. Many different sample types (e.g. water, soils, air, biota, food) have been analysed using these instrumental couplings with AFS detection. These are summarised and discussed.

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Simultaneous determination of twelve inorganic and organic arsenic compounds by liquid chromatography–ultraviolet irradiation–hydride generation atomic fluorescence spectrometry

Cited by 64 publications

References 35 publications

Determination of Arsenic Species in Marine Samples by HPLC-ICP-MS

Determination of Arsenic Species in Marine Samples by HPLC-ICP-MS

Speciation of five arsenic species (arsenite, arsenate, MMAAV, DMAAV and AsBet) in different kind of water by HPLC-ICP-MS

Atomic Fluorescence Spectrometry: a suitable detection technique in speciation studies for arsenic, selenium, antimony and mercury

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