Simultaneous determination of two Monascus metabolites in red yeast rice by HPLC using fluorescence detection

Huang, Zhibing; Xu, Youlong; Hong, Zhang; Li, Laisheng; He, Qinghua; Li, Yanping

doi:10.1016/j.foodchem.2011.01.004

Cited by 9 publications

(6 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…as materials, and the natural MRP products in industrial scale were only the aqueous or ethanol extracts of fermented products, so their components were extremely complex and difficult to be identified. In order to characterize chemical structure of new MRP, many methods had been applied such as TLC, reversed‐phase HPLC, ionic liquid‐nonionic surfactant aqueous two‐phase system, reverse micellar extraction and macroporous resins (MACR) adsorption, UHPLC‐DAD‐QToF‐MS, Integrated Fermentation System, Transcriptomic Analysis, etc . Those methods were time‐consuming, costly, and needed expensive equipment, therefore, some simple methods should be explored to know the general information of red pigments and citrinin in those products.…”

Section: Introductionmentioning

confidence: 99%

A New Method to Purify Hydroxyl Monascus Red Pigment by Retrogradation of Both Pigments and Maize Amylopectin

Lian

Jiang

et al. 2019

Starch - Stärke

View full text Add to dashboard Cite

In monascus red pigment (MRP) products, there exist many components such as polypeptides, amino acids, polysaccharide, and citrinin (toxin). The existence of those components lowers the purity of pigments and brings a great security problem. Hydroxyl MRP has been found in products of China and other countries. This paper presents a new method to isolate hydroxyl MRP by retrograded maize amylopectin (MA) and proposes the mechanism involved based on investigation of Xray‐diffraction, IR and 13C solid NMR spectra. The research results have shown that the optimum retrogradation parameters for purification of hydroxyl MRP are as follows: the ratio of pigments and MA is 1:1, autoclaving temperature 120 °C and time, 20 min, aging temperature 4 °C and time needed, 48 h. Most non‐pigment components without hydroxyls in the samples are dissolved in solution when retrograded MA containing MRP has been hydrolyzed by amylase with only MA and MRP left in the precipitatate. Such precipitation can redissolve in aqueous KOH to open hydrogen bond and the MA in solution can be distilled by ethanol in neutral pH, and then the purified pigments in solution can be concentrated and dried. Every 100 g MA can isolate 68.0 g MRP. The results of IR and 13C solid NMR have indicated that hydrogen bonds might form between C7 of pigment and C1 of MA, also, C10 of pigment and C3 or C2 of MA. Retrogradation is surely a simple, inexpensive, and valid method to isolate pigments with hydroxyl groups and citrinin.

show abstract

Section: Introductionmentioning

confidence: 99%

A New Method to Purify Hydroxyl Monascus Red Pigment by Retrogradation of Both Pigments and Maize Amylopectin

Lian

Jiang

et al. 2019

Starch - Stärke

View full text Add to dashboard Cite

show abstract

“…reported two methods for the simultaneous measurement of monacolins and citrinin by HPLC and micellar electrokinetic capillary chromatography. Recently, our group also reported the determination of two orange pigments and strong blue fluorescent compounds by HPLC . MPA and MPB are analogous in structure but differ in their molecular weight by 28 as a result of the presence of two CH 2 moieties in the side chain .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, our group also reported the determination of two orange pigments and strong blue fluorescent compounds by HPLC. 35,36 MPA and MPB are analogous in structure but differ in their molecular weight by 28 as a result of the presence of two CH 2 moieties in the side chain. 29,30 The structures of MPA and MPB were also analogous to Monascus pigments.…”

Section: Introductionmentioning

confidence: 99%

Determination of two potential toxicity metabolites derived from the disruption of the pksCT gene in Monascus aurantiacus Li As3.4384

Huang

et al. 2017

J Sci Food Agric

Self Cite

View full text Add to dashboard Cite

show abstract

“…Some quantified components of Xuezhikang rather than the quantification methods applied have been reported in the literature. The methods used include HPLC for Xuezhikang [27] , [28] , as well as micellar electrokinetic capillary chromatography [29] , nuclear magnetic resonance (NMR) evaluation [30] , and chromatography coupled mass spectrometry technology to detect several components such as monacolins, pigments and citrinin in red yeast rice [31] , [32] , [33] , [34] . However, no method was reported for quantification of total active ingredients of Xuezhikang by inhibition of HMG-CoA reductase in vitro with HPLC-tandem mass spectrometry (MS/MS).…”

Section: Introductionmentioning

confidence: 99%

Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

Wang

Sun

Sha

et al. 2015

Journal of Pharmaceutical Analysis

View full text Add to dashboard Cite

A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was validated by several intra- and inter-day assays. The production transitions of m/z 147.0/59.1 and m/z 154.0/59.1 were used to detect and quantify mevalonolactone (MVAL) and MVAL-D7, respectively. The accuracy and precision of the method were evaluated over the concentration range of 0.005–1.000 μg/mL for MVAL and 0.010–0.500 μg/mL for lovastatin acid in three validation batch runs. The lower limit of quantitation was found to be 0.005 μg/mL for MVAL and 0.010 μg/mL for lovastatin acid. Intra-day and inter-day precision ranged from 0.95% to 2.39% and 2.26% to 3.38% for MVAL, 1.46% to 3.89% and 0.57% to 5.10% for lovastatin acid, respectively. The results showed that the active ingredients in Xuezhikang capsules were 12.2 and 14.5 mg/g, respectively. This assay method could be successfully applied to the quality control study of Xuezhikang capsule for the first time.

show abstract

Simultaneous determination of two Monascus metabolites in red yeast rice by HPLC using fluorescence detection

Cited by 9 publications

References 15 publications

A New Method to Purify Hydroxyl Monascus Red Pigment by Retrogradation of Both Pigments and Maize Amylopectin

A New Method to Purify Hydroxyl Monascus Red Pigment by Retrogradation of Both Pigments and Maize Amylopectin

Determination of two potential toxicity metabolites derived from the disruption of the pksCT gene in Monascus aurantiacus Li As3.4384

Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

Contact Info

Product

Resources

About