Simultaneous Determination of Uric Acid and Ascorbic Acid Using Edge Plane Pyrolytic Graphite Electrodes

Abstract

Edge plane pyrolytic graphite electrodes have been applied for the determination of uric acid and ascorbic acid. The separate determination of uric acid was found to produce three linear ranges from 100 nM to 3400 mM with a detection limit of 30 nM found to be possible. Uric acid detection was also explored in the presence of 200 mM ascorbic acid where a detection limit of 52 nM was found to be possible. The detection of ascorbic acid in the presence of uric acid was also explored over three linear ranges of ascorbic acid with a limit of detection of 80 nM. Last the simultaneous determination of both uric acid and ascorbic acid is investigated over the range 100 nM to 1000 mM where detection limits of 50 nM and 120 nM were obtained respectively. Analysis of uric acid in a growth tissue medium was found to be successful, confirming the applicability of the methodology to real matrices. This protocol is shown to provide low detection limits, easy handling (no electrode modification), good voltammetric peak separation of uric acid and ascorbic acid and a wide linear dynamic range.

Manganese Dioxide Graphite Composite Electrodes: Application to the Electroanalysis of Hydrogen Peroxide, Ascorbic Acid and Nitrite

“…The calibration curve exhibited a linear range up to 2.5 mM with a limit of detection (based on 3σ) for ascorbic acid of 0.4 μM. This detection limit is lower or comparable to the ones obtained with existing methodologies [35][36][37][38] that range from 0.08 to 71 μM as shown in Table 2.…”

Simultaneous Detection of Ascorbic Acid and Hydrogen Peroxide by Flow‐Injection Analysis with a Thin Layer Dual‐Electrode Detector

“…In spite of these drawbacks, the fact that AA and H 2 O 2 are both electroactive compounds makes quite attractive their detection by electrochemical methods, in view of their inherent selectivity and sensitivity. Moreover, they offer in situ and instantaneous multicomponent measurements, almost always in the same environment in which the analytes of interest normally exist, with on line capabilities and low-cost instrumentation.. On the other hand, the possibility of adopting successfully specific approaches for tackling the electroanalytical monitoring of reciprocally interfering species was already rather abundantly reported [12,14,[26][27][28][29][30].…”

“…They are mostly based on titrimetric [5], chromatographic [6,7], spectrometric [6,[8][9][10], enzymatic [11][12][13] and electroanalytical [6,12,[14][15][16][17][18] approaches. Almost all of these methods suffer from interference from several species [19], among which hydrogen peroxide (H 2 O 2 ) is by far the worst.…”

Selective Detection of Uric Acid in the Presence of Ascorbic Acid and Dopamine Using Polymerized Luminol Film Modified Glassy Carbon Electrode

“…Among many methods for determination of UA in biological samples, voltammetric method has shown to be a powerful tool [9,10]. Recently, edge plane pyrolytic graphite electrodes [11], Ir-modified carbon working electrode with immobilizing uricase [12], hexacyanoferrate lanthanum film modified electrode [13], composite film of polyaniline nanonetworks/p-aminobenzene sulfonic acid modified GCE [14] and mesoporous SiO 2 -modified carbon paste electrodes [15] have been applied for the determination of UA. However, a new method for selective detection of UA in the presence of DA and AA is still required.…”