Simultaneous determination of vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices using liquid chromatography-tandem mass spectrometry

Andriguetti, Nadine Bordin; Lisboa, Letícia Loss; Hahn, Siomara Regina; Pagnussat, Lidiane Riva; Antunes, Marina Venzon; Linden, Rafael

doi:10.1016/j.jpba.2018.12.023

Cited by 31 publications

(35 citation statements)

References 16 publications

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“…As mentioned previously, VCN has some ionisable functional groups, so the retention mechanism of this drug is dependent on both pH and organic modifier. LC analysis under gradient method for quantifying VCN in biological samples has been also reported in some previous studies [9,12,21,23].…”

Section: Chromatographic Conditionmentioning

confidence: 56%

“…To this end, recent studies have investigated a variety of chromatographic methods, using different detectors, to quantify VCN in biological tissues. Liquid chromatography (LC) coupled with mass spectrometric detection (MS/MS) has been utilised [9]. However, despite its high sensitivity, LC-MS/MS requires highly expensive, sophisticated instrumentation, suffers from limited sample throughput and may give rise to ion suppression [10].…”

Section: Introductionmentioning

confidence: 99%

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A sensitive HPLC-UV method for quantifying vancomycin in biological matrices: Application to pharmacokinetic and biodistribution studies in rat plasma, skin and lymph nodes

Ramadon

Courtenay

Permana

et al. 2020

Journal of Pharmaceutical and Biomedical Analysis

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Vancomycin (VCN) is an antibiotic used in the treatment of methicillin-resistant Staphylococcus aureus (MRSA)-derived infections. As it has a relatively narrow therapeutic window, it is imperative to develop a sensitive and reliable analytical method for drug monitoring and pharmacokinetic purposes. In the present study, quick sample preparations and a sensitive high-performance liquid chromatography method using UV detection (HPLC-UV) have been developed and validated. The analytical method for detection and quantification of VCN in rat plasma, skin and lymph node samples was validated based on the Food and Drug Administration (FDA) and European Medicine Agency (EMEA) bioanalytical method validation guidelines. The optimised plasma sample preparation involved a simple protein precipitation step, with extraction recovery of 100.3 ± 0.92%. VCN in all biological matrices was analysed in a HPLC-UV system (215 nm) using a Cortecs ® C18 column (4.6 × 150 mm, 2.7 μm particle size) fitted with a guard cartridge set at 20°C. Reverse phase HPLC under gradient conditions, with mobile phase consisting of 20 mM phosphate buffer containing 0.5% v/v of triethylamine and a mixture of methanol -acetonitrile (70:30, v/v), and runtime of 12 min/sample was employed. The calibration standards used for plasma ranged between 0.1-50 μg/ml, while in the skin and lymph node matrices, standards ranged between 0.05-50 μg/ml with correlation coefficients (R 2 ) of ≥ 0.9995 for all matrices. The method was selective, sensitive, accurate and precise for detecting and quantifying VCN in the biological matrices used. The validated method was successfully utilised in the detection of VCN in a pharmacokinetic and organ biodistribution study carried out in rats following oral and IV bolus administration of the drug. This validated bioanalytical method offers a wide range of potential applications in clinical therapeutic drug monitoring, pharmacokinetics and toxicology.

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Section: Chromatographic Conditionmentioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

A sensitive HPLC-UV method for quantifying vancomycin in biological matrices: Application to pharmacokinetic and biodistribution studies in rat plasma, skin and lymph nodes

Ramadon

Courtenay

Permana

et al. 2020

Journal of Pharmaceutical and Biomedical Analysis

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“…LC-MS/MS assay was developed for the simultaneous determination of Vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices (VAMS). Estimation approaches were used for the measurements of Vancomycin and creatinine as their concentrations measured in VAMS differ from those in plasma [77] .…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for the determination of certain antibiotics used in critically ill patients

Ibrahim

El-Adl

Baraka

et al. 2020

J Pharm Biopharm Res

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“…Antibiotic treatment requires attainment of specific concentrations to achieve optimal outcomes, but there is often a balance between efficacy and toxicity that requires the use of TDM [11,24]. Sample collection for TDM is limited in pediatric patients for multiple reasons.…”

Section: 28mentioning

confidence: 99%

“…In the outpatient setting, timing of sampling relative to doses and travel to collection sites imposes challenges. Along with these obstacles, shipping and handling of liquid samples to testing facilities can be costly and complicated, due to the need for dry ice and constant temperature monitoring [24].…”

Section: 28mentioning

confidence: 99%

Development and validation of a volumetric absorptive microsampling- liquid chromatography mass spectrometry method for the analysis of cefepime in human whole blood: Application to pediatric pharmacokinetic study

Moorthy

Vedar

Zane

et al. 2020

Journal of Pharmaceutical and Biomedical Analysis

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Cefepime is a fourth-generation cephalosporin antibiotic with an extended spectrum of activity against many Gram-positive and Gram-negative bacteria. There is a growing need to develop sensitive, small volume assays, along with less invasive sample collection to facilitate pediatric pharmacokinetic clinical trials and therapeutic drug monitoring. The volumetric absorptive microsampling (VAMS™) approach provides an accurate and precise collection of a fixed volume of blood (10 μL), reducing or eliminating the volumetric blood hematocrit assay-bias associated with the dried blood spotting technique. We developed a high-performance liquid chromatographic method with tandem mass spectrometry detection for quantification of cefepime. Sample extraction from VAMS™ devices, followed by reversed-phase chromatographic separation and selective detection using tandem mass spectrometry with a 4 minute runtime per sample was employed. Standard curves were linear between 0.1 -100 μg/mL for cefepime. Intra

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Simultaneous determination of vancomycin and creatinine in plasma applied to volumetric absorptive microsampling devices using liquid chromatography-tandem mass spectrometry

Cited by 31 publications

References 16 publications

A sensitive HPLC-UV method for quantifying vancomycin in biological matrices: Application to pharmacokinetic and biodistribution studies in rat plasma, skin and lymph nodes

A sensitive HPLC-UV method for quantifying vancomycin in biological matrices: Application to pharmacokinetic and biodistribution studies in rat plasma, skin and lymph nodes

Analytical methods for the determination of certain antibiotics used in critically ill patients

Development and validation of a volumetric absorptive microsampling- liquid chromatography mass spectrometry method for the analysis of cefepime in human whole blood: Application to pediatric pharmacokinetic study

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