Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection

Benturquia, Nadia; Parrot, Sandrine; Sauvinet, Valérie; Renaud, Bernard; Denoroy, Luc

doi:10.1016/j.jchromb.2004.03.061

Cited by 50 publications

(38 citation statements)

References 29 publications

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“…The determination of the antiepileptic drug vigabatrin, Glu, Asp and GABA was also performed using an identical buffer. The method was used for monitoring vigabatrin and amino acid neurotransmitters in microdialysates from the rat striatum during intracerebral infusion of the drug and revealed rapid vigabatrininduced changes in GABA and Glu levels (Benturquia et al, 2004). Then five neurostransmitters which were separated and quantitated using CE/LIF and NDA Figure 3.…”

Section: Labelling and Analysis By Capillary Electrophoresismentioning

confidence: 99%

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Rammouz

Lacroix

Garrigues

et al. 2007

Biomedical Chromatography

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This paper reviews analytical methods, instrumental developments and applications for derivatization of primary amines with naphthalene-2,3-dicarboxaldehyde using fluorescence and chemiluminescence detection with capillary electrophoresis (CE) and high performance liquid chromatography (HPLC). The use of lasers as well as lamps as the excitation source for fluorescence detection is discussed. The detection limit observed with naphthalene-2,3-dicarboxaldehyde derivatization is often lower and better than those obtained with other analytical separations and other fluorescent dyes. In addition, this paper describes the crucial points that influence the stability of NDA primary amine derivatives, and summarize the separation, derivatization and migration conditions of the different techniques, with their advantages and drawbacks.

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Section: Labelling and Analysis By Capillary Electrophoresismentioning

confidence: 99%

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Rammouz

Lacroix

Garrigues

et al. 2007

Biomedical Chromatography

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“…In order to monitor simultaneously a drug and several neurotransmitters in the brain extracellular fluid, Benturquia et al [58] used CE-LIF coupled to in vivo microdialysis sampling. Determination of the antiepileptic drug vigabatrin and the amino acid neurotransmitters, Glu, Asp, and GABA, was performed on low-concentration samples which were derivatized with NDA and separated using a pH 9.2, 75 mM sodium borate running buffer containing 60 mM SDS, and 5 mM HP-b-CD.…”

Section: Neurochemical Applications Of Cementioning

confidence: 99%

Recent advances in amino acid analysis by capillary electrophoresis

et al. 2006

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This paper describes a number of articles that have been published on amino acid analysis using CE during the period from June 2003 to May 2005. This review article follows the previous ones of Smith (Electrophoresis 1999, 20, 3078-3083), Prata et al. (Electrophoresis 2001, 22, 4129-4138), and Poinsot et al. (Electrophoresis 2003, 24, 4047-4062). Several new developments in amino acid analysis with CE are reported concerning UV detection, LIF, MS, and NMR. In addition, we describe articles concerning clinical and pharmaceutical studies, neuroclinical applications, and agricultural and food analysis.

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“…This paper, however, carries out analyte detection at very low wavelength (202 nm) and is thus prone to several endogenous and exogenous interferences. Two CE methods for the analysis of VGB in biological fluids comprise LIF detection after derivatization [21,22] to reach the necessary sensitivity and selectivity. They include derivatization with naphthalene-2,3-dicarboxaldehyde and the use of a 442 nm laser [21] or 5-carboxymethylrhodamine succinimidyl ester with a 543.5 nm laser [22], respectively.…”

Section: Introductionmentioning

confidence: 99%

“…Two CE methods for the analysis of VGB in biological fluids comprise LIF detection after derivatization [21,22] to reach the necessary sensitivity and selectivity. They include derivatization with naphthalene-2,3-dicarboxaldehyde and the use of a 442 nm laser [21] or 5-carboxymethylrhodamine succinimidyl ester with a 543.5 nm laser [22], respectively. Benturquia et al [21] analyze VGB in microdialysates from rat brain by micellar EKC (MEKC), including the addition of hydroxypropyl-b-CD to the BGE; moreover, the matrix is dialysed extracellular fluid and is thus quite different from human plasma.…”

Section: Introductionmentioning

confidence: 99%

Determination of vigabatrin in human plasma by means of CE with LIF detection

et al. 2007

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A method has been developed for the quantitation of the antiepileptic drug vigabatrin (VGB) in human plasma. It is based on CE with LIF detection. The effect of the pH of the buffer and of N-methylglucamine (GLC) as BGE constituent was investigated. The final BGE consisted of 50 mM borate buffer, pH 9.0, with 100 mM GLC and enabled separation within 12 min at 20 kV voltage. An SPE procedure was used for the pretreatment of biological samples, based on mixed-mode lipophilic-cation exchange cartridges, followed by a derivatization step with 6-carboxyfluorescein-N-succinimidyl ester (CFSE). Fluorescence was excited by an Ar-ion laser (lambda(exc) = 488 nm). Linearity was observed in the 10-120 microg/mL plasma concentration range. Extraction yield was >96%, precision (expressed as RSD) <6.7% and accuracy (recovery) was between 97.0 and 101.6%. The method has been successfully applied to the analysis of VGB in plasma of epileptic patients undergoing therapy with the drug.

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Simultaneous determination of vigabatrin and amino acid neurotransmitters in brain microdialysates by capillary electrophoresis with laser-induced fluorescence detection

Cited by 50 publications

References 29 publications

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

The use of naphthalene‐2,3‐dicarboxaldehyde for the analysis of primary amines using high‐performance liquid chromatography and capillary electrophoresis

Recent advances in amino acid analysis by capillary electrophoresis

Determination of vigabatrin in human plasma by means of CE with LIF detection

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