Simultaneous Determination of α-Glucosidase Inhibitory Triterpenoids in Psidium guajava Using HPLC–DAD–ELSD and Pressurized Liquid Extraction

Abstract

Psidium guajava, a popular food and medicine dual purposes plant cultivated in tropical and subtropical regions, has been widely used as food crop and folk medicine, such as anti-diabetes agent, around the world. Triterpenoids have been considered as the major active ingredients of P. guajava. In the present study, a high-performance liquid chromatography coupled with diode array and evaporative light scattering detectors (HPLC–DAD–ELSD) method was developed for simultaneous determination of nine triterpenoids in P. guajava. Pressurized liquid extraction (PLE) was performed for sample preparation, and the analysis was achieved on a Cosmosil 5C18-MS-II (Nacalai Tesque, Kyoto, Japan) column eluted with gradient 0.1% aqueous formic acid-methanol system. The drift tube temperature of ELSD was set at 40 °C, and nitrogen flow-rate was at 1.6 L/min. All calibration curves for the analytes showed good linear regression (R2 > 0.9992) within test ranges. The established method was validated for intra-day and inter-day precisions (RSDs < 5%) and accuracy (recovery 94.23–106.87%). The validated method was successfully applied to determinate nine triterpenoids in 15 samples from the leave or fruit of P. guajava. In addition, the α-glucosidase inhibition assay showed good α-glucosidase inhibition activity in almost all the determined triterpenoids. The present study suggested that triterpenoids should be the quality control markers for P. guajava and HPLC–DAD–ELSD was an effective tool for the quality control of P. guajava.

Chemical Characterization and Hepatoprotective Effects of a Standardized Triterpenoid-Enriched Guava Leaf Extract

“…CA was identified as the most abundant triterpenoid in TGL. Our result was in agreement with a previously reported study on the major triterpenoids in guava leaves . Previous studies, including our group work, has reported that GUB is the major phenolic component present in guava. , The HPLC–DAD-MS analysis revealed that most phenolics including guavinoside B, flavonoids, and oleuropeic acid-conjugated phenolic glycosides in guava leaves were successfully removed from TGL (see Supporting Information Figure S4).…”

Advancements in Drug Delivery Systems for Natural Compounds Targeting Metabolic Disorders Associated with Inflammation

“…Taking a different administration route, scientists devised nanofiber patches for the transdermal delivery of curcumin. The transdermal route has gained recognition as an alternative to conventional oral delivery for various drugs, with numerous studies indicating improved bioavailability via this route [64][65][66][67][68][69][70][71][72][73]. These nanofibers were created using polyvinyl alcohol and gelation, resulting in a formulation characterized by fiber diameters of 200-250 nm and high reproducibility [74][75][76][77][78].…”

Etnofarmacologia, Fitoquímica, Toxicologia E Atividades Anti-Inflamatória E Antinociceptiva De Folhas De Psidium Guajava L.: Uma Revisão

“…Diversos triterpenos e esteroides foram identificados em derivados da espécie P. guajava, incluindo ácidos ursólico e oleanólico e 20-hidroxi-derivados, ácidos crataegoico, jacumárico, crataególico, guajanoico, asiático, guavacumárico, goreishico, isoneruicumárico, guavênico e guaijavólico, ilelatifol D, uvaol e β-sitosterol e seu glicosídeo (2,5,27,(183)(184)(185)(186)(187)(188). Os meroterpenoides floroglucinol policíclicos guajamer A-H, psidial D-G, um meroterpenoide benzoilfloroglucinol metilado guajamer I, e dois análogos foram isolados das folhas da espécie vegetal (189)(190)(191).…”