Simultaneous determination of β-lactam antibiotics in milk by ion-pair liquid chromatography

Liquid chromatographic assay for dicloxacillin in plasma

“…The available methods include microbiological assays, enzyme immunoassays, which require relatively long time for analysis or they do not have the required sensitivity [1,2] and high-performance liquid chromatography (HPLC) assays. The available HPLC methods for dicloxacillin reported include methods which are limited by complex requirements for sample preparation or by the use of chromatographic modifiers as well as methods for non-biological applications such as quality control [3][4][5][6]. Dicloxacillin (Fig.…”

Determination of Beta-Lactam residues in milk by high performance liquid chromatography

“…The adjustment of the extract pH between 8.1 and 8.3, before the elution, was important to avoid clogging of C18 cartridge (Boison and Keng, 1998). The use of C18 SPE cartridge for purification of extracts containing beta-lactams has been described by many authors (Terada and Sakabe, 1985;Munns et al, 1985;Boison et al, 1994;Tarbin et al, 1995;Sorensen et al, 1997;Luo et al, 1997;Boison and Keng, 1998;Takeba et al, 1998) and was also appropriate in this method. The 1 mL volume of acetonitrile:water 50:50 v/v was sufficient to extract the beta-lactams completely.…”

A Rapid Validated RP-HPLC Method for the Simultaneous Determination of Cleaning Validation and Cross Contamination of 12 Beta-Lactam Compounds

“…Fukutsu et al reported a RPLC-MS method for the verification of cefmetazole and cefpodoxime proxetil contamination in other pharmaceuticals [5]. Takeba et al reported the iron-pair liquid chromatography method for the determination of beta-lactam antibiotics in milk [6]. Also, some capillary electrophoresis methods are available for the determination of beta-lactam compounds [7, 8].…”