Simultaneous Electrochemical Determination of Glutathione and Glutathione Disulfide at a Nanoscale Copper Hydroxide Composite Carbon Ionic Liquid Electrode

Abstract

Direct simultaneous electrochemical determination of glutathione (GSH) and glutathione disulfide (GSSG) has been presented using a nanoscale copper hydroxide carbon ionic liquid composite electrode. To the best of our knowledge, this is the first report on the simultaneous determination of these two biologically important compounds based on their direct electrochemical oxidation. Incorporation of copper(II) hydroxide nanostructures in the composite electrode results in complexation of Cu(II) with the thiol group of GSH and leads to a significant decrease in GSH oxidation overpotential, while an anodic peak corresponding to the direct oxidation of GSSG as the product of GSH oxidation is observed at higher overvoltages. Low detection limits of 30 nM for GSH and 15 nM for GSSG were achieved based on a signal-to-noise ratio of 3. The proposed method is free from interference of cysteine, homocysteine, ascorbic acid (AA), and uric acid (UA). No electrode surface fouling was observed during successive scans. Stability, high sensitivity, and low detection limits made the proposed electrode applicable for the analysis of biological fluids.

Rapid and sensitive determination of free thiols by capillary zone electrophoresis with near‐infrared laser‐induced fluorescence detection using a new BODIPY‐based probe as labeling reagent

“…Up till now, various methods for the determination of thiol compounds have been used, especially UV-Vis spectrophotometry [8,9], spectrofluorimetry [10,11], electrochemical method [12][13][14], MS [15,16], LC [17,18], and CE [19][20][21].…”

Ascorbic acid-functionalized Ag NPs as a probe for colorimetric sensing of glutathione

“…Therefore, it is highly desirable to develop a new sensitive, fast and practical method for the detection of GSH in biological samples. Although conventional analytical tools including electrospray ionization mass spectrometry (ESI-MS) (Kailasa et al 2012), matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) (Wan et al 2013), high-performance liquid chromatography (HPLC) (Casey et al 2002), fluorescence spectrometry (Shao et al 2010) and electrochemistry (Safavi et al 2009) could meet the demand for the sensitive and selective detection of GSH in biological samples, all these methods are expensive, time-consuming and require tedious sample preparations. In this frame, it is necessary to develop a facile and sensitive method for the analysis of GSH in various samples.…”

Electrochemical determination of nonylphenol based on ionic liquid-functionalized graphene nanosheet modified glassy carbon electrode and its interaction with DNA

“…Ionic liquid (ILs) has attracted much attention in the fields of electrochemical sensors fabrication due to its specific properties such as negligible vapor pressure, wide electrochemical windows, and good solubility [30][31][32]. Many materials were prepared using ILs as template solvents and exhibited large surface areas and good electrocatalytic performance [33,34].…”