Simultaneous electrochemical magneto genosensing of foodborne bacteria based on triple-tagging multiplex amplification

Brandão, Delfina; Liébana, Susana; Campoy, Susana; Cortés, Pilar; Alegret, Salvador; Pividori, María Isabel

doi:10.1016/j.bios.2015.07.008

Cited by 29 publications

(20 citation statements)

References 29 publications

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“…The double-tagged DNA from E. coli O157: H7 was achieved by PCR performed with a double-tagging set of primers for the amplification of the eaeA (151 bp) gene fragment specific to E. coli (Brandão et al, 2015b), being each primer labeled in 5′ with biotin (to achieve the immobilization on the magnetic-MIP) and digoxigenin (for the optical readout by using the optical reporter anti-DIG-HRP), as schematically shown in Fig. 1 G4 biotin-dendrimer, followed by the incubation with streptavidin-Cy5.…”

Section: Characterization Of the Binding Of Double-tagged Dna From E Coli O157:h7 By A Magneto-actuated Immunoassaymentioning

confidence: 99%

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Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules

Aissa

Herrera-Chacón

Pupin

et al. 2017

Biosensors and Bioelectronics

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Magnetic separation based on biologically-modified magnetic particles is a preconcentration procedure commonly integrated in magneto actuated platforms for the detection of a huge range of targets. However, the main drawback of this material is the low stability and high cost. In this work, a novel hybrid molecularly-imprinted polymer with magnetic properties is presented with affinity towards biotin and biotinylated biomolecules. During the synthesis of the magneto core-shell particles, biotin was used as a template. The characterization of this material by microscopy techniques including SEM, TEM and confocal microscopy is presented. The application of the magnetic-MIPs for the detection of biotin and biotinylated DNA in magneto-actuated platforms is also described for the first time. The magnetic-MIP showed a significant immobilization capacity of biotinylated molecules, giving rise to a cheaper and a robust method (it is not required to be stored at 4°C) with high binding capacity for the separation and purification under magnetic actuation of a wide range of biotinylated molecules, and their downstream application including determination of their specific targets.

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Section: Characterization Of the Binding Of Double-tagged Dna From E Coli O157:h7 By A Magneto-actuated Immunoassaymentioning

confidence: 99%

“…The characterization of a double-tagged DNA amplicon from E. coli O157: H7 obtained by double-tagging PCR (Brandão et al, 2015b) was also performed by a magneto-actuated immunoassay, as schematically shown in Fig. S8.…”

Section: Fractional Occupancy Biotin Hrp / Biotin Hrp Kdmentioning

confidence: 99%

Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules

Aissa

Herrera-Chacón

Pupin

et al. 2017

Biosensors and Bioelectronics

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“…The (bio)modified‐beads are then collected after analyte interactions, stimulating the redox processes. The integration of magnetic beads to NC‐CPEs gives further advantages in terms of separation of the analyte from complex matrix and enhancement of biological reactions, with promising applications in food safety, environmental monitoring and bioengineering . This strategy opens up alternatives to develop specific magnetic‐nano(bio)fluids where the (bio)modifier is carried on the magnetic nanoparticles without the necessity to modify the NC‐CPE.…”

Section: Strategies To Bio‐functionalized Nc‐cpesmentioning

confidence: 99%

Customized Bio‐functionalization of Nanocomposite Carbon Paste Electrodes for Electrochemical Sensing: A Mini Review

Muñoz

Baeza

2017

Electroanalysis

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Over the past few decades, the (bio)functionalization of carbon nanomaterials (CNMs), such as nanohorns, carbon nanotubes, graphene, graphite and related with a wide range of (bio)modifiers have been extensively studied for their incorporation on different pure metal or carbon electrode surfaces via drop‐casting. However, CNMs are also shown to be important functional additives for polymers, having great potential to produce rigid nanocomposite materials with a range of enhanced properties, including mechanical, optical, electrical, thermal and electrochemical. The high malleability derived from the host polymer allows alternative strategies that can be carried out in order to incorporate different types of (bio)modifiers in/on/into a polymeric nanocomposite electrode. Accordingly, this mini review overviews the main methodologies used for the bio‐functionalization of electrochemical transducers based on nanocomposite carbon paste electrodes (NC‐CPEs). Additionally, the most extensively (bio)modifiers used in electrochemical (bio)sensing, together with their various electrocatalytical performance are also discussed, fact that might serve as a general outlook for planning further research.

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“…BioMag®, Dynabeads®, Adembeads® and Miltenyi®) and are widely used in laboratories to extract desired biological components, such as cells, organelles or DNA, from a fluid. In recent years, the magnetic properties of MPs have also been used as labels [ 30 ] and bioreceptor platforms in biosensing [ 31 ]. As shown in Figure 3 (left), they consist of an inorganic core of iron oxide (magnetite (Fe 3 O 4 ), maghemite (Fe 2 O 3 ) or other insoluble ferrites) coated with a polymer to confer stability (e.g .…”

Section: Protein Stabilisationmentioning

confidence: 99%

Bioconjugation and stabilisation of biomolecules in biosensors

Liébana

Drago

2016

Essays in Biochemistry

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Suitable bioconjugation strategies and stabilisation of biomolecules on electrodes is essential for the development of novel and commercially viable biosensors. In the present review, the functional groups that comprise the selectable targets for practical bioconjugation methods are discussed. We focus on describing the most common immobilisation techniques used in biosensor construction, which are classified into irreversible and reversible methods. Concerning the stability of proteins, the two main types of stability may be defined as (i) storage or shelf stability, and (ii) operational stability. Both types of stability are explained, as well as the introduction of an electrophoretic technique for predicting protein–polymer interactions. In addition, solution and dry stabilisation as well as stabilisation using the covalent immobilisation of proteins are discussed including possible factors that influence stability. Finally, the integration of nanomaterials, such as magnetic particles, with protein immobilisation is discussed in relation to protein stability studies.

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Simultaneous electrochemical magneto genosensing of foodborne bacteria based on triple-tagging multiplex amplification

Cited by 29 publications

References 29 publications

Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules

Magnetic molecularly imprinted polymer for the isolation and detection of biotin and biotinylated biomolecules

Customized Bio‐functionalization of Nanocomposite Carbon Paste Electrodes for Electrochemical Sensing: A Mini Review

Bioconjugation and stabilisation of biomolecules in biosensors

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