Simultaneous enhancement of phenolic compound degradations by Acinetobacter strain V₂ via a step‐wise continuous acclimation process

Abstract

Phenol degradation enhancement of Acinetobacter strain V2 by a step-wise continuous acclimation process was investigated. At the end of 8 months, three stable adapted strains, designated as R, G, and Y, were developed with the sub-lethal concentration of phenol at 800, 1100, and 1400 mg/L, respectively, from 400 mg/L of V2 parent strain. All strains degraded phenol at their sub-lethal level within 24 h, their growth rate increased as the acclimation process continued and retained their degradation properties even after storing at -80 °C for more than 3 years. All adapted strains appeared coccoid with an ungranulated surface under electron microscope compared to typical rod-shaped parental strain V2 . The adapted Y strain also possessed superior degradation ability against aniline, benzoate, and toluene. This study demonstrated the use of long term acclimation process to develop efficient and better pollutant degrading bacterial strains with potentials in industrial and environmental bioremediation.

Analysis of a phenol-adapted microbial community: degradation capacity, taxonomy and metabolic description

“…The presence of harmful molecules, like phenol, in the environment favours the prevalence of resistant micro-organisms in the microbial communities through one or more of the following mechanisms: induction of specific enzymes which enhance degradative capacities; development of a specific microbial subpopulation with capacity for co-metabolic process with the main microbial population; and the selection of mutants that acquired altered enzymatic specificities or novel metabolic activities. (Spain and Van Veld 1983;Timmis and Pieper 1999;Ojo 2007;Ray and Peters 2010;Gu et al 2016;Lin et al 2016). Consortium MR-01 was acclimated to phenol for 3 months; in response to this adaptation, the maximum phenol degradation was attained faster than in other works reported in literature (Saravanan et al 2008;Kilic ß and D€ onmez 2013;Lim et al 2013;Gu et al 2016;Bera et al 2017).…”

“…; Lin et al . ). Consortium MR‐01 was acclimated to phenol for 3 months; in response to this adaptation, the maximum phenol degradation was attained faster than in other works reported in literature (Saravanan et al .…”

Comparative Genomic and Transcriptomic Analysis of Phenol Degradation and Tolerance in Acinetobacter lwoffii through Adaptive Evolution

“…The degradation of phenol at a high concentration can rely solely on pure species such as Burkholderia sp. (1.5 g/L, approximately 48 h) [24], R. opacus PD630 (1.5 g/L, 45 h) [12], B. brevis (1.5 g/L, 108 h) [8], Acinetobacter strain V2 (1.4 g/L, approximately 24 h) [14], C. albicans TL3 (1.41 g/L, 50 h) [25], and C. tropicalis PHB5 (2.4 g/L, ~48 h) [26]. The degradation concentration of A. lwoffii NL115 was lower than that of C. tropicalis PHB5, but its degradation efficiency (125 mg/L/h) was higher.…”

“…The phenol degradation capacity of Rhodococcus opacus [12], Rhodococcus pyridinivorans [13], Acinetobacter sp. [14], Chlorella sp. [15], and Is Chrysis galbana Parke [16] has been improved using ALE.…”

Draft genome sequence of phenol degrading Acinetobacter sp. Strain V2, isolated from oil contaminated soil

“… 8 Acinetobacter sp. V2 strain is able to degrade 400 ppm of phenol within 24 h. 9 Presence of commercially important proteins apart from its ability to degrade diesel and engine oil makes this organism unique and thus genome sequencing.…”