Simultaneous estimation of amlodipine besilate and olmesartan medoxomil in pharmaceutical dosage form

Abstract

Two UV Spectrophotometric and one reverse phase high performance liquid chromatography methods have been developed for the simultaneous estimation of amlodipine besilate and olmesartan medoxomil in tablet dosage form. First UV spectrophotometric method was a determination using the simultaneous equation method at 237.5 nm and 255.5 nm over the concentration range 10-50 μg/ml and 10-50 μg/ml, for amlodipine besilate and olmesartan medoxomil with accuracy 100.09%, and 100.22% respectively. Second UV spectrophotometric method was a determination using the area under curve method at 242.5-232.5 nm and 260.5-250.5 nm over the concentration range of 10-50 μg/ml and 10-50 μg/ml, for amlodipine besilate and olmesartan medoxomil with accuracy 100.10%, and 100.48%, respectively. In reverse phase high performance liquid chromatography analysis carried out using 0.05M potassuim dihydrogen phosphate buffer:acetonitrile (50:50 v/v) as the mobile phase and Kromasil C18 (4.6 mm i.d.×250 mm) column as the stationery phase with detection wavelength of 238 nm. Flow rate was 1.0 ml/min. Retention time for amlodipine besilate and olmesartan medoxomil were 3.69 and 5.36 min, respectively. Linearity was obtained in the concentration range of 4-20 μg/ml and 10-50 μg/ml for amlodipine besilate and olmesartan medoxomil, respectively. Proposed methods can be used for the estimation of amlodipine besilate and olmesartan medoxomil in tablet dosage form provided all the validation parameters are met.

Development and Validation of Dual Wavelength UV Spectrophotometric Method for simultaneous estimation of Cilnidipine and Olmesartan Medoxomil in Tablet dosage form

“…The absorbance of various dilutions of CIL and OLME in diluents was measured at λ 2 and λ 3 . At these two wavelength absorbance difference of CIL at any concentration level was found to be zero while for OLME, absorbance difference was found to increase concomitantly as concentration was increase [6][7][8][9][10][11].…”

Development of Spectrophotometric Method for Quantitative Estimation of Amlodipine Besylate, Olmesartan Medoxomil and Hydrochlorthiazide in Tablet Dosage Form

“…Amlodipine besylate is official in IP [2] and BP [3] and Hydrochlorthiazide is official in IP [2], BP [3] and USP [4]. Literature survey revealed that various methods such as UV [9,10,15,16,19,20], HPLC [11,17], HPTLC [12,18], LC-MS/MS [13] and UPLC [14] are available in single and combination with other drugs. However, no spectrophotometric method has yet been reported for simultaneous estimation of amlodipine besylate, olmesartan medoxomil and hydrochlorthiazide in tablet dosage forms.…”

Novel Analytical Method using Acquity QDa Mass Detector Coupled with LC-PDA for Impurity Profiling of Amlodipine Besylate and Olmesartan Medoxomil in Fixed Dose Tablets Formulation

“…The literature survey reveals that, few analytical methods are available for the assay of ADB /OSM in tablets by spectrophotometric techniques [8][9][10], few articles by the HPLC methods for ADB /OSM tablets [11][12][13] and one method which describe the estimation of impurities alone which was published on combination product by using UPLC technique [14]. However, UPLC technique is very costly and complex instrument having very tedious procedure.…”