Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC

Parveen, Rabea; Ahmad, Farhan Jalees; Iqbal, Zeenat; Singh, Mhaveer; Kamal, Y. T.; Ahmad, Sayeed

doi:10.1556/achrom.26.2014.2.13

Cited by 11 publications

(12 citation statements)

References 8 publications

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“…Presence of picrosides [ 15 – 17 ], cucurbitacins [ 18 – 20 ], apocynin [ 21 ], and their anticancer potential [ 18 , 20 , 22 – 34 ] has already been reported and hence simultaneous analysis of these compounds in the BAF was carried out using newly developed HPTLC methods. These methods were developed as per ICH guidelines, similar to several methods reported by the Laboratory for Quality Control of Herbal Drugs and Botanicals [ 35 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

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HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract ofPicrorhiza kurroa

Mallick

Singh

Parveen

et al. 2015

BioMed Research International

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Objective. Hydroalcoholic extract of Picrorhiza kurroa and its fractions were subjected to in vitro screening for cytotoxicity; further best active fraction (BAF) obtained was tested against Ehrlich ascites carcinoma (EAC) model in Balb/c mice after its quality control analysis. Methods. Cytotoxicities of all the fractions and mother extract of P. kurroa were determined, using MTT assay on breast cancer (MCF-7, MDA-MB 231) and cervical cancer (HeLa, SiHa) cell lines. Metabolic fingerprinting was developed using HPTLC with quantification of biomarkers (cucurbitacins B and E; betulinic acid; picrosides 1 and 2; and apocynin) in BAF. The EAC tumor-bearing mice were used for in vivo anticancer activity after oral administration (50 mg Kg−1) for 10 days. Results. Cytotoxicity assay of mother extract and its fractions over breast cancer and cervix cancer cell lines showed that dichloromethane (DCM) fraction was most cytotoxic (IC50 36.0–51.0 µg mL−1 at 72 h). Oral administration of DCM fraction showed significant reduction in tumor regression parameters, viable tumor cell count and restoration of hematological parameters may be due to presence of cucurbitacins B and E; betulinic acid; picrosides 1 and 2; and apocynin, as compared to the untreated mice of the control group. Conclusion. The DCM fraction of P. kurroa displayed potent anticancer activity and can be further explored for the development of a potential candidate for cancer therapy.

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Section: Methodsmentioning

confidence: 99%

“…The newly developed HPTLC method was optimized and validated as per the ICH guidelines for calibration, linearity, precision, accuracy, robustness, specificity, LOD, and LOQ, similar to the other methods reported from this laboratory [ 35 , 36 ].…”

Section: Methodsmentioning

confidence: 99%

HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract ofPicrorhiza kurroa

Mallick

Singh

Parveen

et al. 2015

BioMed Research International

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“…21,22 Calibration curve and linearity Different volumes (0.1 1 µL/spot) of the standard solutions (oleanolic acid and swertiamarin) were separately spotted on TLC plates (in triplicate) to obtain different concentrations of oleanolic acid (200-2000 ng/spot) Leishmaniasis is a wide spread life-threatening disease which is caused by obligate intracellular, kinetoplastid protozoa of the genus Leishmania. 15 It is considered one of the serious tropical diseases in many countries.…”

Section: Validation Of the Methods Developedmentioning

confidence: 99%

“…The data of peak area versus drug concentration were treated by linear least square regression and the concentration range showing best regressions was considered for linearity. 22,23 Precision Precision of the proposed method was obtained by repeatability and intermediate precision. Inter-day and intra day precisions were done by preparing and applying three different concentrations of standards (in triplicate) on the same day and on three different consecutive days, respectively.…”

Section: Extractionmentioning

confidence: 99%

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HPTLC Analysis and Antiproliferative Effect of Various Extracts of Swertia alata on Growth of Leishmania donovani Promastigotes in vitro

Bajaj¹,

Wakode²,

Khan³

2017

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The present study was carried out to evaluate the quality control analysis and antiproliferative effects of petroleum ether, chloroform, ethanol and aqueous extracts of Swertia alata (family Gentianaceae) on Leishmania donovani. A basic, exact, quick and reproducible high performance thin layer chromatography (HPTLC) has been created for synchronous analysis of Oleanolic acid and Swertiamarin from S. alata. The method was validated for linearity, precision, specificity, accuracy and robustness. Densitometric scanning of the plates directly at 254nm was used for analysis of Swertiamarin, while as for the analysis of Oleanolic acid; plates were scanned for 520 nm after spraying with anis aldehyde-sulphuric acid reagent. For the extract of plants and Amphotericin B, the concentration response curve was plotted and IC 50 values were determined and also MTT assay was carried out for comparative study. Compact bands of Oleanolic acid and Swertiamarin were obtained at R f 0.62±0.001 and 0.66±0.002, linearity was satisfactory for oleanolic acid and swertiamarin. The developed and validated HPTLC methods were having Linearity range 200-2000 and 100-2000 ng/spot for oleanolic acid and swertiamarin respectively with r 2 value 0.989±0.003 and 0.999±0.03, limit of detection (11.25 and 6.19 ng/spot), limit of quantification (37.51 and 20.64 ng/spot), recovery (98.4-101.0 % and 98.2-100.8%) and precision with % RSD < 2%. Various dried extracts were found to contain oleanolic acid in the range of 0.009%-0.012% and swertiamarin in the range of 0.113-0.135% (w/w). Cytotoxicity assay of extracts over promastigotes showed that ethanolic extract was found to be most cytotoxic (IC 50 31.43±1.90 g/mL) after 96 h in comparison to other extracts. The ethanolic extract showed significant reduction in promastigotes which may be due to presence of oleanolic acid as compared with the other groups. The present HPTLC method is being reported for the first time and can be used for routine quality control. Key words: Antiproliferative, HPTLC, Oleanolic acid, Quality control, Swertiamarin. 14 Oleanolic acid and swertiamarin are very important bioactive constituents of S. alata; therefore, there is an urgent need to develop a simple method for estimation of two chemotherapeutic compound using sophisticated instruments like high performance liquid chromatography (HPTLC). Till date, several analytical methods have been reported for analysis of these both the compounds. However, literature survey shows no single quantitative method available for estimation of both the compounds in the plant S. alata. Sakshi Bajaj et al.: HPTLC analysis and antiproliferative effect of various extracts of Swertia alata S108Pharmacognosy Journal, Vol 9, Issue 6 (Suppl), Nov-Dec, 2017 well closed containers under refrigeration until used for the biological testing. Preparation of standard and quality control (QC) samplesStock solutions of Oleanolic acid and Swertiamarin (10mg/mL) were prepared in HPLC grade methanol and by appropriate dilution standard soluti...

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Multiple shoot induction and jasmonic versus salicylic acid driven elicitation for enhanced andrographolide production in Andrographis paniculata

Zaheer

Giri

2015

Plant Cell Tiss Organ Cult

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Simultaneous estimation of anti-cancer terpenoids in pharmaceutical nanoformulation by RP-HPLC and HPTLC

Cited by 11 publications

References 8 publications

HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract ofPicrorhiza kurroa

HPTLC Analysis of Bioactivity Guided Anticancer Enriched Fraction of Hydroalcoholic Extract ofPicrorhiza kurroa

HPTLC Analysis and Antiproliferative Effect of Various Extracts of Swertia alata on Growth of Leishmania donovani Promastigotes in vitro

Multiple shoot induction and jasmonic versus salicylic acid driven elicitation for enhanced andrographolide production in Andrographis paniculata

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