Simultaneous Estimation of Paracetamol, Ambroxol Hydrochloride, Levocetirizine Dihydrochloride, and Phenylephrine Hydrochloride in Combined Tablet Formulation by First-Order Derivative Spectrophotometry

Abstract

Paracetamol, ambroxol hydrochloride, levocetirizine dihydrochloride, and phenylephrine hydrochloride are used in combination for the treatment of chronic sinusitis, rhinitis, fever, nasal discharge, sore throat, and wheezing. The present work deals with method development for simultaneous estimation of paracetamol, ambroxol hydrochloride, levocetirizine dihydrochloride, and phenylephrine hydrochloride in tablet formulation by first-order derivative spectrosphotometry. For determination of sampling wavelength, 10 μg/mL of each of paracetamol, ambroxol hydrochloride, levocetirizine dihydrochloride, and phenylephrine hydrochloride was scanned in 200–400 nm ranges and sampling wavelengths were found to be 305.5 nm for paracetamol, 321 nm for ambroxol hydrochloride, 244 nm for levocetirizine dihydrochloride, and 280 nm for phenylephrine hydrochloride in first-order derivative spectrophotometry. In this method, linearity was observed in the ranges of 20–140 μg/mL for paracetamol and 10–70 μg/mL for ambroxol hydrochloride, levocetirizine dihydrochloride, and phenylephrine hydrochloride. The % recovery was within the range between 98 and 102%, and % relative standard deviation for precision and accuracy of the method was found to be less than 2%. The method is validated as per International Conference on Harmonization Guidelines. The method can be successfully applied for the simultaneous analysis of these drugs in pharmaceutical dosage forms.

Application of a nanostructured sensor based on graphene‐ and ethyl 2‐(4‐ferrocenyl[1,2,3]triazol‐1‐yl)acetate‐modified carbon paste electrode for determination of methyldopa in the presence of phenylephrine and guaifenesin

“…Phenylephrine decreases the congestion and the production of mucus thereby reducing discomfort and making breathing easier . To date, just a few methods have been introduced in the literature for the determination of phenylephrine, most of which are spectrophotometric or chromatographic methods and electroanalytical techniques have been rarely reported …”

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“…Literature survey revealed that there are so many methods of analysis were reported for individual analysis of Levocetirizine Dihydrochloride, Phenylephrine hydrochloride, and Paracetamol, as well as with combination with other drugs [7][8][9][10][11][12][13][14][15][16][17][18][19] , but no method is reported for Simultaneous determination of Levocetirizine Dihydrochloride, Phenylephrine hydrochloride and Paracetamol in combination formulations. A proposed analytical method has been developed and validated as per ICH guideline 20 .…”

Simultaneous Determination of Paracetamol and Tramadol in Pharmaceutical Tablets by Derivative UV-Vis Absorption Spectrophotometry

“…Determination of Paracetamol has been performed using various methods like reversed-phase high performance liquid chromatographic (RP-HPLC) with caffeine [1], in Pharmaceutical Mixture Using HPLC and GC-MS [15], by first-order derivative spectrophotometery in combination with ambroxol hydrochloride, levocetirizine dihydrochloride, and phenylephrine hydrochloride [16]. By RP-HPLC in combination with Chlorzoxazone, and Nimesulide [17].…”