Simultaneous estimation of ramipril, acetylsalicylic acid and atorvastatin calcium by chemometrics assisted UV-spectrophotometric method in capsules

Abstract

In the present work, three different spectrophotometric methods for simultaneous estimation of ramipril, aspirin and atorvastatin calcium in raw materials and in formulations are described. Overlapped data was quantitatively resolved by using chemometric methods, viz. inverse least squares (ILS), principal component regression (PCR) and partial least squares (PLS). Calibrations were constructed using the absorption data matrix corresponding to the concentration data matrix. The linearity range was found to be 1-5, 10-50 and 2-10 µg mL -1 for ramipril, aspirin and atorvastatin calcium, respectively. The absorbance matrix was obtained by measuring the zero-order absorbance in the wavelength range between 210 and 320 nm. A training set design of the concentration data corresponding to the ramipril, aspirin and atorvastatin calcium mixtures was organized statistically to maximize the information content from the spectra and to minimize the error of multivariate calibrations. By applying the respective algorithms for PLS 1, PCR and ILS to the measured spectra of the calibration set, a suitable model was obtained. This model was selected on the basis of RMSECV and RMSEP values. The same was applied to the prediction set and capsule formulation. Mean recoveries of the commercial formulation set together with the figures of merit (calibration sensitivity, selectivity, limit of detection, limit of quantification and analytical sensitivity) were estimated. Validity of the proposed approaches was successfully assessed for analyses of drugs in the various prepared physical mixtures and formulations.

Simultaneous Estimation of Hydrochlorothiazide and Losartan Potassium in Bulk Solid Dosage Form by Chemometric Assisted Spectrophotometric Methods

“…[5] Also can be used for the descriptive issue solving like the model composition, identification and understanding. Chemometrics shows its application in the multivariate data collection and analysis.…”

Simultaneous Estimation of Atorvastatin and Aspirin in Bulk and Capsule Dosage Form by Chemometric Assisted Spectrophotometric Methods

“…Literature survey revealed that very few analytical methods like UVSpectroscopy and HPLC methods were reported and no chemometric method was reported for the analysis of the above said combination. [11][12][13][14][15][16][17][18][19][20][21][22][23] Moreover, most of the proposed methods demand complex extraction procedures for the separation of drugs in to individual components from the dosage form Thus the present study aims to design chemometric assisted spectroscopic methods for the intricate analysis of Atorvastatin and Aspirin without prior separation.…”

Development and Validation of an Alternate Stability-indicating UV Spectrophotometric Analytical Method for Aspirin in Tablets

“…In addition, a literature survey exposed there were numerous analytical methodologies to detect ASP and SA in tablets and biofluids [6][7][8][9][10][11][12][13][14][15][16][17] . Particularly, for the pharmaceutical industry, the interest raises on the need of developing robust analytical stability methods to ensure drugs will not degrade until the sell-by date has expired [18,19] .…”

“…However, with the introduction of multicomponent methods, UV spectrophotometry showed an astonishing capability in the quantitation of compounds in mixtures without previous separation [8][9][10][11][15][16][17]24] . This aspect revealed great advantage over HPLC methods, such as lower cost, less time of analysis and minimum organic solvent waste.…”