Simultaneous Estimation of Twenty Eight Phenolic Compounds by a Novel and Expeditious Method Developed on Quaternary Ultra-Performance Liquid Chromatography System with a Photodiode Array Detector

Abstract

Plant secondary metabolites including phenolics and flavonoidsare synthesized through phenylpropanoid and phenylpropanoid-acetate pathways and significantly contribute against adverse effect of abiotic and biotic stresses. Herein, we present the development and execution of a novel and expeditious ultra-performance liquid chromatographic-photodiode array (UPLC-PDA) method for qualitative and quantitative analysis of 28 phenolic compounds comprising of flavonoids, phenolic acids, aldehydes and alcohols. The method is able to separate phenolic compounds in just 17 min with the separation of isobaric species such as 3,4 dihydroxybenzoic acid and 3,5 dihydroxy benzoic acid; quercetin and taxifolin. Linear curves concentrations ranged from 6-18 µg/mL (3,5 dihydroxy benzoic acid), 4-12 µg/mL (catechin and salicylic acid) and 2-6 µg/mL for rest of the compounds and correlation coefficients were >0.994. The limit of detection (LOD) varied from 0.04-0.45 µg/mL. Cotton root samples were used to assess the method in terms of recovery efficiency (85-120%), precision (0.12-4.09%) and intermediate precision (0.32-4.0%).Phenolics and flavonoidsin root samples of healthy and diseased plants as well as leaf samples of healthy plants were successfully quantified using this novel method without an expensive Mass Spectrometer.

Optimization through a Box–Behnken Experimental Design of the Microwave-Assisted Extraction of the Psychoactive Compounds in Hallucinogenic Fungi (Psylocibe cubensis)

“…High-performance liquid chromatography with fluorescence (FLR) [ 27 ], mass spectrometer (MS) [ 28 ], or photodiode array detection (PDA) have been the most widely used method for the detection of psilocybin and psilocin [ 23 ]. The last one registers the UV-Vis spectra of all the outgoing compounds under several wavelengths, so that compounds can be detected under optimal absorption conditions [ 29 ].…”

UV-Vis Spectrophotometry and UPLC–PDA Combined with Multivariate Calibration for Kappaphycus alvarezii (Doty) Doty ex Silva Standardization Based on Phenolic Compounds

“…Chromatographic techniques such as high-performance liquid chromatography (HPLC) and ultra-performance liquid chromatography (UPLC) have been developed for phenolic compounds determination in plant sources [7,8]. The UPLC-PDA technique has successfully characterized the phenolic acids and flavonoids from herbal plants [9]. Additionally, the UPLC-PDA was employed to characterize and quantify the phenolic compounds in K. alvarezii due to a faster separation than the HPLC.…”