Simultaneous Evaluation of Chemiluminescence and Bioluminescence in a Phagocytic System

Abstract

The spectra of luminol-dependent chemiluminescence of leukocytes, forming as a result of their oxygen-dependent bactericidal systems activation, and bacterial bioluminescence of Escherichia coli recombinant strain with cloned lux operon, used as the object of phagocytosis, are not identical. Mutual overlapping of these spectra reaches 87%, including overlapping of the photoemission maximums. However these spectra can be evaluated separately in the short-wave "arm" of the chemiluminescence spectrum (<420 nm) and the long-wave "arm" of the bioluminescence spectrum (>560). The kinetics of luminol-dependent chemiluminescence of phagocytes and of bacterial bioluminescence in their mixtures is characterized by mutually dependent phase-wise changes in the intensity of the analyzed parameters.

Development of a Fully Automated Flow Injection Analyzer Implementing Bioluminescent Biosensors for Water Toxicity Assessment

“…V. fischeri strain NRRL-11177 (Dr Lange S.A.) was grown in 20 mL DSMZ No 6904 broth [ 4 ] for 20 h in an orbital incubator at 190 rpm at 24 °C [ 3 ]. The thus developed stock culture was mixed with 30% glycerin, dispensed in 2 mL vials and stored at −70 °C.…”

“…Bioassays are methods for assessing the toxic impact of whole samples on the environment and for screening environmental samples before going onto detailed chemical analyses that can be time consuming, expensive and do not allow monitoring [ 2 ]. The utilization of organisms possessing lux—genes [ 3 ] gained significant importance during the last decade since toxicity bioassays have been recognized as essential tests along chemical analyses [ 4 ]. The widely used marine photobacterium Vibrio fischeri is a self—maintained luminescent unit.…”