Simultaneous genotyping of the three lactose tolerance-linked polymorphisms LCT –13907C&gt;G, LCT –13910C&gt;T and LCT –13915T&gt;G with Pyrosequencing™ technology

Nilsson, Torbjörn; Olsson, Lovisa

doi:10.1515/cclm.2008.015

Cited by 19 publications

(15 citation statements)

References 15 publications

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“…Sequencing was performed using a PSQ96 SNP reagent Kit and a PSQ 96MA system (Pyrosequencing AB) PSQ96MA 2.0.1 software. The procedure has been previously described in detail (21, 22). …”

Section: Methodsmentioning

confidence: 99%

Body fat and dairy product intake in lactase persistent and non-persistent children and adolescents

Almon¹,

Patterson²,

Nilsson³

et al. 2010

Food & Nutrition Research

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BackgroundLactase non-persistent (LNP) individuals may be lactose intolerant and therefore on a more restricted diet concerning milk and milk products compared to lactase persistent (LP) individuals. This may have an impact on body fat mass.ObjectiveThis study examines if LP and LNP children and adolescents, defined by genotyping for the LCT-13910 C > T polymorphism, differ from each other with regard to milk and milk product intake, and measures of body fat mass.DesignChildren (n=298, mean age 9.6 years) and adolescents (n=386, mean age 15.6 years), belonging to the Swedish part of the European Youth Heart Study, were genotyped for the LCT-13910 C > T polymorphism. Dietary intakes of reduced and full-fat dairy varieties were determined.ResultsLNP (CC genotype) subjects consumed less milk, soured milk and yoghurt compared to LP (CT/TT genotype) subjects (p<0.001). Subsequent partitioning for age group attenuated this observation (p=0.002 for children and p=0.023 in adolescents). Six subjects were reported by parents to be ‘lactose intolerant’, none of whom were LNP. LNP children and adolescents consumed significantly less reduced fat milk and milk products than LP children and adolescents (p=0.009 for children and p=0.001 for adolescents).ConclusionsWe conclude that LP is linked to an overall higher milk and dairy intake, but is not linked to higher body fat mass in children and adolescents.

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“…Sequencing was performed using a PSQ96 SNP reagent Kit and a PSQ 96MA system (Pyrosequencing AB) PSQ96MA 2.0.1 software. The procedure has been previously described in detail (21, 22). …”

Section: Methodsmentioning

confidence: 99%

Body fat and dairy product intake in lactase persistent and non-persistent children and adolescents

Almon¹,

Patterson²,

Nilsson³

et al. 2010

Food & Nutrition Research

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“…LCT-13907C>G and LCT-13915T>G, have been described [23]. These genotypes were not tested for in the actual study.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism in the oxytocin promoter region in patients with lactase non-persistence is not related to symptoms

et al. 2009

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BackgroundOxytocin and the oxytocin receptor have been demonstrated in the gastrointestinal (GI) tract and have been shown to exert physiological effects on gut motility. The role for oxytocin in the pathophysiology of GI complaints is unknown. The aim of this study was to examine genetic variations or polymorphism of oxytocin (OXT) and its receptor (OXTR) genes in patients with GI complaints without visible organic abnormalities.MethodsGenetic variants in the OXT promoter region, and in the OXTR gene in DNA samples from 131 rigorously evaluated patients with Irritable Bowel Syndrome (IBS), 408 homozygous subjects referred for lactase (LCT-13910 C>T, rs4988235) genotyping, and 299 asymptomatic blood donors were compared. One polymorphism related to the OXT gene (rs6133010 A>G) and 4 related to the OXTR gene (rs1465386 G>T, rs3806675 G>A, rs968389 A>G, rs1042778 G>T) were selected for genotyping using Applied Biosystems 7900 HT allele discrimination assays.ResultsThere were no statistically significant differences in the genotype or allele frequencies in any of the SNPs when IBS patients were compared to healthy controls. Among subjects referred for lactase genotyping, the rs6133010 A>G OXT promoter A/G genotype tended to be more common in the 154 non-persistent (27.3%) subjects than in the 254 lactase persistant (18.1%) subjects and in the healthy controls (19.4%) (p = 0.08). When direct comparing, the A/G genotype was less common in the OXT promoter region in controls (p = 0.09) and in subjects with lactase persistence (p = 0.03) compared to subjects with lactase non-persistence. When healthy controls were viewed according to their own LCT-13910 genotypes, the C/C lactase non-persistent controls had a higher frequency for the OXT promoter A/G genotype than LCT-13910 T/T lactase persistent controls (41.2% vs 13.1%).No significant differences in frequencies of the investigated OXTR SNPs were noted in this study.ConclusionThe results suggest that polymorphism in the promoter region of the OXT gene is most common in subjects with lactase non-persistence. This polymorphism may not be related to GI symptoms, as it is related to lactase non-persistence also in healthy controls.

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“…Sequencing was performed using a PSQ96 SNP reagent Kit and a PSQ 96MA system (Pyrosequencing AB) PSQ 96MA 2.0.1 software. The procedure has been previously described in detail (24, 25). …”

Section: Methodsmentioning

confidence: 99%

Lactase persistence and milk consumption are associated with body height in Swedish preadolescents and adolescents

Almon¹,

Nilsson

Sjöstróm³

et al. 2011

Food & Nutrition Research

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BackgroundBody height is a classic polygenic trait. About 80%–90% of height is inherited and 10%–20% owed to environmental factors, of which the most important ones are nutrition and diseases in preadolescents and adolescents.ObjectiveThe aim of this study was to explore potential relations between the LCT (lactase) C>T-13910 polymorphism, milk consumption, and body height in a sample of Swedish preadolescents and adolescents.DesignIn a cross-sectional study, using a random sample of preadolescents and adolescents (n = 597), dietary intakes were determined. Anthropometric measurements including sexual maturity (Tanner stage) and birth weight were assessed. Parental body height and socio-economic status (SES) were obtained by questionnaires. Genotyping for the LCT C>T-13910 polymorphism that renders individuals lactase persistent (LP) or lactase non-persistent (LNP) was performed by DNA sequencing. Stepwise backward multivariate linear regression was used.ResultsMilk consumption was significantly and positively associated with body height (β = 0.45; 95% CI: 0.040, 0.87, p = 0.032). Adjustments were performed for sex, parental height, birth weight, body mass index (BMI), SES, and Tanner stage. This model explains 90% of the observed variance of body height (adjusted R2 = 0.89). The presence of the -13910 T allele was positively associated with body height (β = 2.05; 95% CI: 0.18, 3.92, p = 0.032).ConclusionsMilk consumption is positively associated with body height in preadolescents and adolescents. We show for the first time that a nutrigenetic variant might be able to explain in part phenotypic variation of body height in preadolescents and adolescents. Due to the small sample size further studies are needed.

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Simultaneous genotyping of the three lactose tolerance-linked polymorphisms LCT –13907C>G, LCT –13910C>T and LCT –13915T>G with Pyrosequencing™ technology

Abstract: Pyrosequencing is a robust genotyping modality suitable for clinical genotyping of patients not only of European, but also of African or Middle Eastern descent, who may harbor any combination of the three LCT mutations, LCT -13907C>G, LCT -13910C>T, LCT -13915T>G.

Cited by 19 publications

References 15 publications

Body fat and dairy product intake in lactase persistent and non-persistent children and adolescents

Body fat and dairy product intake in lactase persistent and non-persistent children and adolescents

Polymorphism in the oxytocin promoter region in patients with lactase non-persistence is not related to symptoms

Lactase persistence and milk consumption are associated with body height in Swedish preadolescents and adolescents

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