Simultaneous High Performance Liquid Chromatographic Determination of Dapsone and Monoacetyldapsone in Human Plasma and Urine

Ma, Aijin; Ym, Irshaid; Al-Hadidi, H. F.; Nm, Rawashdeh

doi:10.1111/j.1365-2710.1991.tb00311.x

Cited by 6 publications

(1 citation statement)

References 12 publications

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“…Several methods for the quantification of dapsone have been related in the literature. These studies were focused on identification of degradation products (Bardsley et al, 2011), evaluation of stability (Nahata et al, 2000), quantification of dapsone and its metabolites in muscle, plasma (Kwadijk and Torano, 2002) and urine (Abuirjeie et al, 1991) in biological evaluation of the drug in humans or animals (Hela et al, 2003). Apart of these, a method for quantification of dapsone in theospheres, a special lipid nanoparticulated system, has been developed (Santos et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Validation of analytical method by HPLC for determination of dapsone in polymeric nanocapsules based on crude rice brain oil

2017

J App Pharm Sci

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Dapsone is a sulfone used for the treatment of skin diseases such as psoriasis. Its clinical use by the oral route is limited. For counteract this hindrance, innovative systems based on polymeric nanocapsules containing rice bran oil and dapsone have been developed. Thus, an analytical method using HPLC for quantification of dapsone in polymeric nanocapsules based on rice brain oil was developed and validated. The separation was achieved on a C18 reverse phase column (Phenomenex, 4.6 × 150 mm, 5 μm) using a mobile phase composed of acetonitrile-1.5 % acetic acid (25:75 v/v) at a flow rate of 0.7 mL.min −1 . The injection volume was 20 μL and the wavelength of detection was 230 nm. The method was linear in the range of 5-25 µg.mL −1 , with a correlation coefficient of 0.9999. Precision and accuracy analysis showed low relative standard deviation (lower than 2) and recovery percentage in the range of 99.72 -106.25%. The limits of detection and quantification were 0.41 and 1.24 μg.mL −1 , respectively. The analytical method was specific, linear, precise and exact, showing that this procedure can be applied in quantification of dapsone-loaded polymeric nanocapsules based on rice brain oil.

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Section: Introductionmentioning

confidence: 99%

Validation of analytical method by HPLC for determination of dapsone in polymeric nanocapsules based on crude rice brain oil

2017

J App Pharm Sci

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Acetylator phenotypes of Jordanian diabetics

Irshaid

Al-Hadidi

et al. 1992

Eur J Clin Pharmacol

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The acetylator phenotype was determined in 31 insulin-dependent (IDDM) and 110 noninsulin-dependent (NIDDM) Jordanian diabetics, and was compared to that of 160 healthy volunteers of the same ethnic group. Dapsone was used as the test drug. The rapid acetylator phenotype was slightly less frequent in IDDM and slightly more frequent in NIDDM. Neither of the differences was significant. When acetylator status in the two types of diabetes mellitus was compared, there was a significant difference among the two groups. Patients with IDDM had a higher percentage of the slow acetylator phenotype when compared to NIDDM patients. The association between acetylator status and IDDM in Jordanians, which agrees with that reported for the Saudi Arabian population, is the reverse of what is found in European populations. The results demonstrate ethnic differences in acetylator status among IDDM patients.

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Laboratory diagnosis of poisonings

Wennig¹

1996

Human Toxicology

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Simultaneous High Performance Liquid Chromatographic Determination of Dapsone and Monoacetyldapsone in Human Plasma and Urine

Cited by 6 publications

References 12 publications

Validation of analytical method by HPLC for determination of dapsone in polymeric nanocapsules based on crude rice brain oil

Validation of analytical method by HPLC for determination of dapsone in polymeric nanocapsules based on crude rice brain oil

Acetylator phenotypes of Jordanian diabetics

Laboratory diagnosis of poisonings

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