Simultaneous high-performance liquid chromatographic analysis of free carotenoids and carotenoid esters

Zonta, F; Stancher, B.; Marletta, Giuliana

doi:10.1016/s0021-9673(00)96354-4

Cited by 25 publications

(12 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Chlorella tablet (650 mg) contained 416 mg of Chlorella powder with the following nutritional composition (per 100 g): 5.4 g moisture, 60.6 g protein, 3.7 g total carbohydrates, 12.8 g total lipids, 13.0 g dietary fiber, 4.5 g ash, 58,900 IU vitamin A, 74 mg vitamin C, 22.8 mg vitamin E, and 2.4 g chlorophylls [10]. The carotenoid content of Chlorella powder was determined in our laboratory by using a high-performance liquid chromatography (HPLC) method [24] and the results are as follows (per 100 g): 260 mg lutein, 5 mg zeaxanthin, 24 mg α-carotene, and 17 mg β-carotene.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Impact of daily Chlorella consumption on serum lipid and carotenoid profiles in mildly hypercholesterolemic adults: a double-blinded, randomized, placebo-controlled study

Ryu

Lim

Park

et al. 2014

Nutr J

View full text Add to dashboard Cite

BackgroundHigh level of serum cholesterol is considered to be a major risk factor for cardiovascular disease (CVD). A double-blinded, randomized, placebo-controlled trial was performed to test the hypothesis that a daily intake of Chlorella may improve serum lipid profile through enhancement of serum carotenoid concentration in mildly hypercholesterolemic subjects.MethodsEligible subjects (n = 63) were randomized to either Chlorella (5 g/day) or placebo for a double-blinded trial with a 2-week lead-in period and a 4-week intervention period. Serum triglycerides, total cholesterol, lipoproteins, apolipoproteins and carotenoids were assessed at the beginning and the end of the trial.ResultsCompared with the control group, the Chlorella group exhibited remarkable changes in total cholesterol (Chlorella −1.6%; placebo 0.03%; P = 0.036), triglycerides (Chlorella −10.3%; placebo 11.9%; P = 0.002), lutein/zeaxanthin (Chlorella 89.6%; placebo −1.7%; P < 0.0001), and α-carotene (Chlorella 163.6%; placebo 15%; P < 0.0001). Improvement of serum lipids was supported by significant reductions of very low-density lipoprotein cholesterol (Chlorella −11%; placebo 11.8%; P = 0.006), apolipoprotein B (Chlorella −1.5%; placebo 1.7%; P = 0.044), non high-density lipoprotein (Chlorella −2.6%; placebo −0.5%; P = 0.032), and high-density lipoprotein/triglycerides (Chlorella 4.0%; placebo −9.5%; P = 0.023), suggesting an inhibitory effect of Chlorella on the intestinal absorption of dietary and endogenous lipids. Further, the changes of serum lipids appeared to be associated with the changes of serum carotenoids.ConclusionDaily consumption of Chlorella supplements provided the potential of health benefits reducing serum lipid risk factors, mainly triglycerides and total cholesterol, in mildly hypercholesterolemic subjects. The effect was related to carotenoid consumption.Trial registrationWHO International Clinical Trials Registry Platform KCT0000259.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Serum carotenoids were analyzed after deproteinizing with ethanol containing the internal standard (α-tocopherol acetate) and then extracting with hexane. After evaporation, the residue was dissolved in mobile phase and quantified by using HPLC (Shiseido SP 3023) [24]. …”

Section: Methodsmentioning

confidence: 99%

Impact of daily Chlorella consumption on serum lipid and carotenoid profiles in mildly hypercholesterolemic adults: a double-blinded, randomized, placebo-controlled study

Ryu

Lim

Park

et al. 2014

Nutr J

View full text Add to dashboard Cite

show abstract

“…Zonta et al (6) isolated the ester fraction by column chromatography on aluminia; the extract was saponified, and the fatty acids were analyzed after methylation via gas chromatography. The results of this time-consuming procedure showed C14:0, C16:0, C18:0, C18:1, and C18:2 to be the main fatty acids esterifying lutein in marigold.…”

Section: Introductionmentioning

confidence: 99%

Differentiation between Lutein Monoester Regioisomers and Detection of Lutein Diesters from Marigold Flowers (Tagetes erecta L.) and Several Fruits by Liquid Chromatography−Mass Spectrometry

Breithaupt

Wirt

Bamedi

2001

J. Agric. Food Chem.

157

View full text Add to dashboard Cite

Liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCIMS) was employed for the identification of eight lutein monoesters, formed by incomplete enzymatic saponification of lutein diesters of marigold (Tagetes erecta L.) by Candida rugosa lipase. Additionally, the main lutein diesters naturally occurring in marigold oleoresin were chromatographically separated and identified. The LC-MS method allows for characterization of lutein diesters occurring as minor components in several fruits; this was demonstrated by analysis of extracts of cape gooseberry (Physalis peruviana L.), kiwano (Cucumis metuliferus E. Mey. ex Naud.), and pumpkin (Cucurbita pepo L.). The assignment of the regioisomers of lutein monoesters is based on the characteristic fragmentation pattern: the most intense daughter ion generally results from the loss of the substituent (fatty acid or hydroxyl group) bound to the epsilon-ionone ring, yielding an allylic cation. The limit of detection was estimated at 0.5 microg/mL with lutein dimyristate as reference compound. This method provides a useful tool to obtain further insight into the biochemical reactions leading to lutein ester formation in plants.

show abstract

“…Extraction methods for carotenoids in general tend to be difficult, time consuming and prone to error due to oxidation and losses in extraction (Zakaria et al, 1979). Recent reports on HPLC and spectrophotometric measurement of carotenoids have been growing in number (Stewart, 1977;Ranganna, 1979;Fisher and Rouseff, 1986;Bureau and Bushway, 1986;Quackenbush and Smallidge, 1986;Zonta et al, 1987), but have not included corresponding simplifications in extraction.…”

Section: Introductionmentioning

confidence: 99%

Rapid Extraction of Lycopene and β‐Carotene from Reconstituted Tomato Paste and Pink Grapefruit Homogenates

Sadler

Davis

Dezman

1990

Journal of Food Science

405

255

View full text Add to dashboard Cite

Samples were extracted in Erlenmeyer flasks with hexane-ethanolacetone (50:25:25). After extraction, 15 mL of water were added to separate the solution into polar and nonpolar layers. Lycopene was concentrated in the upper nonpolar phase while other components and polar pigments were contained in the lower polar phase. The lycopene phase was analyzed using isocratic HPLC with a C-18 column and methanol-THF-water (67:27:6) mobile phase. Lycopene and l3-carotene eluted as chromatographically pure peaks.

show abstract

Simultaneous high-performance liquid chromatographic analysis of free carotenoids and carotenoid esters

Cited by 25 publications

References 16 publications

Impact of daily Chlorella consumption on serum lipid and carotenoid profiles in mildly hypercholesterolemic adults: a double-blinded, randomized, placebo-controlled study

Impact of daily Chlorella consumption on serum lipid and carotenoid profiles in mildly hypercholesterolemic adults: a double-blinded, randomized, placebo-controlled study

Differentiation between Lutein Monoester Regioisomers and Detection of Lutein Diesters from Marigold Flowers (Tagetes erecta L.) and Several Fruits by Liquid Chromatography−Mass Spectrometry

Rapid Extraction of Lycopene and β‐Carotene from Reconstituted Tomato Paste and Pink Grapefruit Homogenates

Contact Info

Product

Resources

About