Simultaneous high-performance liquid chromatographic assay of the activities of erythrocytic hypoxanthine-guanine phosphoribosyl transferase and purine nucleoside phosphorylase

Halfpenny, Anne P.; Brown, Phyllis R.

doi:10.1016/s0021-9673(01)83783-3

Cited by 15 publications

(3 citation statements)

References 8 publications

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“…Hypoxanthine (Hx) is then produced by the further degradation reaction of IMP when a sufficient enzyme base is available, and further degradation of Hx is the rate-determining step, varying with the type of fish. Consequently, the increased quantities of Hx would determine how fresh the fish is as its increased quantities would mean that fish is stale. , Recognizing this need, Qiong et al fabricated a bilayer coated-wire electrode in which they employed an SF layer on Pt electrode on which was immobilized xanthine oxidase (XOD) is an enzyme for Hx detection. Then a layer of cellulose acetate was coated, which safeguarded the electrode against fouling compounds like proteins.…”

Section: Applicationsmentioning

confidence: 99%

Silk Fibroin As an Immobilization Matrix for Sensing Applications

Prakash

Mane

George

et al. 2021

ACS Biomater. Sci. Eng.

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The development of flexible, biocompatible, and environmentfriendly sensors has attracted a significant amount of scientific interest for the past few decades. Among all the natural materials, silk fibroin (SF), due to its tunable biodegradability, biocompatibility, ease of processing, presence of functional groups, and controllable dimensions, has opened up opportunities for immobilizing multitudinous biomolecules and conformability to the skin, among other attractive opportunities. The silk fibroins also offer good physical properties, such as superior toughness and tensile strength. The sensors made of SF as an immobilization matrix have demonstrated excellent analytical performance, sensing even at low concentrations. The significant advantage of silk fibroins is the presence of functional groups along with a controllable conformation transition that enables immobilization of receptor molecules using silk fibroins as an immobilization matrix enables us to entrap the receptor molecules without using any chemical reagents. This review encompasses a detailed discussion on sensors, the advantages of using silk fibroins as an immobilization matrix for various receptors, their applications, and the future research scope in this state-of-the-art technology based upon the explorable applications for silk fibroin-based sensors.

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Section: Applicationsmentioning

confidence: 99%

Silk Fibroin As an Immobilization Matrix for Sensing Applications

Prakash

Mane

George

et al. 2021

ACS Biomater. Sci. Eng.

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“…17 Various methods have been proposed for the determination of trace amounts of HX, such as paper chromatography, ion exchange chromatography, HPLC, and capillary electrophoresis. [18][19][20][21][22] stripping voltammetry have also been developed as alternative methods, which were based on the direct oxidation of HX or its complexes at the electrode surface. 23,24 XOD can catalyze the oxidation of HX by molecular oxygen to yield uric acid, [25][26][27] so the XOD-based biosensors that measure either the consumed oxygen or the produced hydrogen peroxide and/or uric acid have been demonstrated as the satisfactory sensors for HX.…”

Section: A New Electrochemiluminescent (Ecl) Biosensor Based On An El...mentioning

confidence: 99%

“…The determination of HX is very important for the quality control of fish products in food industries . Various methods have been proposed for the determination of trace amounts of HX, such as paper chromatography, ion exchange chromatography, HPLC, and capillary electrophoresis. − However, these methods require complicated and time-consuming procedures. Polarography and stripping voltammetry have also been developed as alternative methods, which were based on the direct oxidation of HX or its complexes at the electrode surface. , XOD can catalyze the oxidation of HX by molecular oxygen to yield uric acid, − so the XOD-based biosensors that measure either the consumed oxygen or the produced hydrogen peroxide and/or uric acid have been demonstrated as the satisfactory sensors for HX. − Because the presence of hydrogen peroxide can enhance the electrochemiluminescence of luminol, − an electrochemiluminescent (ECL) sensor can thus be developed to determine HX …”

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confidence: 99%

Electrochemiluminescent Biosensor for Hypoxanthine Based on the Electrically Heated Carbon Paste Electrode Modified with Xanthine Oxidase

et al. 2008

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A new electrochemiluminescent (ECL) biosensor based on an electrically heated carbon paste electrode (HCPE) that was surface modified by xanthine oxidase (XOD) was designed and constructed in this work. It was found that the ECL intensity of luminol could be enhanced at the surface of XOD/HCPE by adding hypoxanthine (HX) to the solution, and there was a linear relationship between the ECL intensity and the concentration of HX. On the basis of this, an ECL enzyme biosensor can thus be developed to detect HX. However, because the activity of XOD is highly dependent on temperature, the biosensor is very sensitive to the temperature of the electrode. Also, because the temperature of the electrode may also affect the diffusion and convection of the luminescent compounds near the electrode surface, a suitable temperature for XOD/HCPE has to be controlled to achieve the best ECL signal. The key feature of the designed biosensor is that the temperature of the electrode is controllable so the most suitable temperature for the enzyme reaction can be obtained. The obtained results showed that the ECL enzyme biosensor exhibited the best sensitivity at an electrode temperature of 35 degrees C for the detection of HX. The detection limit was 30-fold lower than that at room temperature (25 degrees C).

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A sensitive xanthine oxidase electrode with silk net for estimating fish freshness

Tian

Yang

Shen³

1996

Chin. J. Chem.

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An enzyme biosensor was constructed using a plate platinum electrode and immobilked xanthine oxidase (XOD). Only a very small quantity of enzyme was chemically immobilized on a special silk net. Hydrogen peroxide released during the enzymatic reaction waa detected by the electrode at +0.65 V (VE. Ag/AgCl). The electrode wbs very sensitive to hypoxanthine and its detection limit was lxlO-' mol/L. W h e n it was applied to the determination of 6sh freshness, the results agreed well with those obtained by traditional methods-determination of total volatile basic nitrogen (TVB-N) and microbial count. A range for estimating the freshness of river 6sh waa suggested. KeywordsXanthine oxidase electrode, biosensor, amperometry, fish freshness Biosensor technology is entering a very active ~h a s e . l -~ In resent years, it was a p plied widely in the growing biotechnology and food processing areas, in the clinical laboratories, and in pollution control. It is very important to estimate the freshness of fish and meat for food industry. Adenqsine triphosphate (ATP) in the fish tissue begins to degrade after the death of a fish, ATP is decomposed quickly to adenosine diphosphate (ADP), adenosine monophosphate (AMP), and inosine monophosphate (IMP). IMP is an important factor of the pleasant flavor of fresh fish. Under the existence of enzymes, IMP is further decomposed to inosine (-), hypoxanthine (Hx), xanthine (X), and uric acid (UA). In general, enzymatic decomposition of inosine or hypoxanthine is the rate-determining step, depending on the kind of fish. Therefore, hypoxanthine is accumulated in fish tissue and its amount can be used to indicate the fish In order to determine hypoxanthine, some complicated methods, such as paper chromatography,8 anion-exchange chr~matography,~ HPLClO and precipitation" have been employed. Recently, some enzyme biosensors have been designed for this purpose. The method was relatively simple and the determination was rapid. In these designs, a basic solid electrode detected consumed oxygen or generated hydrogen peroxide amperometricdy during the following enzymatic reactions: Hypoxanthine + 1 / 2 0 2 + H20 xzD Xanthine + H202 XOD Xanthine + 1/202 + HzO Uric acid + H202

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Simultaneous high-performance liquid chromatographic assay of the activities of erythrocytic hypoxanthine-guanine phosphoribosyl transferase and purine nucleoside phosphorylase

Cited by 15 publications

References 8 publications

Silk Fibroin As an Immobilization Matrix for Sensing Applications

Silk Fibroin As an Immobilization Matrix for Sensing Applications

Electrochemiluminescent Biosensor for Hypoxanthine Based on the Electrically Heated Carbon Paste Electrode Modified with Xanthine Oxidase

A sensitive xanthine oxidase electrode with silk net for estimating fish freshness

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