Simultaneous high-performance liquid chromatographic determination of the antiretroviral agents amprenavir, nelfinavir, ritonavir, saquinavir, delavirdine and efavirenz in human plasma

Conventional HPLC Method Used for Simultaneous Determination of the Seven HIV Protease Inhibitors and Nonnucleoside Reverse Transcription Inhibitor Efavirenz in Human Plasma

“…[1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] However, these methods have several disadvantages in terms of cost performance, time consumption and necessary equipment; for example, the use of expensive disposable cartridges at the solid-phase drug extraction, gradient elution control by a gradient HPLC pump system, and the ultraviolet detection at multiple wavelengths.…”

“…Both interday and intraday CVs for all drugs were less than 8.5%, which is similar to or much lower than previously reported values. [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15] Mean extraction recoveries varied from 81.2% (IDV) to 98.4% (APV). These results indicate that the method developed here achieves a high degree of reproducibility and accuracy.…”

Efavirenz Does Not Interact with the ABCB1 Transporter at the Blood—Brain Barrier

“…EFV was extracted from samples and quantified following an HPLC method adapted from a previously described method (22). Briefly, the HPLC system consisted of a Shimatzu pump (LC-10AS; Shimadzu, Croissy-Beaubourg, France) and an auto sampler (Waters 717 Plus; Waters, St. Quentin en Yvelines, France).…”

A sensitive and robust lc-ms/ms method with monolithic column and electrospray ionization for the quantitation of efavirenz in human plasma: application to a bioequivalence study

“…This is because of its long half-life, which means effective concentrations are maintained for long periods. 3 Several methods are described in literature for the quantification of efavirenz in biological fluids by high performance liquid chromatography with ultraviolet detection (HPLC-UV), [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22] HPLC with fluorescence detector, 23 liquid chromatography with mass spectrometry detection (LC-MS), 24 liquid chromatography with mass spectrometry in tandem detection(LC-MS/MS) [25][26][27][28][29] and matrix assisted laser desorption/ionization with time-of-flight in tandem detection (MALDI-TOF/ TOF). 30 When sample throughput is an important issue, such as in pharmacokinetic applications, the development of rugged methods with short analysis times becomes an important consideration.…”