Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice

Sachin, B.S.; Koul, Maharaj K.; Zutshi, Anup; Singh, Shashank; Tikoo, Ashok K.; Tikoo, Manoj Kumar; Saxena, Ajit Kumar; Sharma, Shrestha; Johri, R.K.

doi:10.1016/j.jchromb.2007.12.015

Cited by 15 publications

(8 citation statements)

References 18 publications

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“…Besides the nutritional value, C. deodara was reported to possess many pharmacological activities. It had been reported that C. deodara could alleviate symptoms of tic (Sachin et al., ). A previous study showed that C. deodara had free radical scavenging activity (Tiwari et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…Cedrus deodara (Roxb.) Loud (family: pinaceae), commonly named Himalayan cedar, has been employed in both traditional Chinese medicine and international natural medicine (Sachin et al, 2008;Zhang et al, 2009a). Pine needles of C. deodara has long been considered as an herbal remedy for the prevention and treatment of various diseases and metabolic diseases (Kar et al, 1975;Singh et al, 2007;Zhang et al, 2009a;Kumar et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Wang

et al. 2016

Animal Physiology Nutrition

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The aim of the study was to evaluate the effect of Chinese herbal mixture (CHM) in laying hen diets on performance, egg quality and egg nutrition components, as well as selected blood biochemical parameters. A total of 270 Lohmann Brown laying hens, 24 week of age, were randomly distributed into three dietary treatments and fed for 6 week. The CHM was used at the levels of 0%, 0.5% and 1.0% in the diets of the control and two treatment groups respectively. Each dietary treatment was divided into five replicates, comprising of 18 hens each. Hens were housed in a 3-tier battery system. Feed and water were provided ad libitum. Blood samples and eggs were collected at the end of the experiment. The results showed that dietary treatments did not affect egg weight, mortality, eggshell thickness, albumen height, Haugh units, eggshell-breaking strength and egg-shape index. Dietary supplementation of CHM significantly improved (p < 0.05) laying rate and the lowest feed conversion ratio were observed with 1.0% CHM supplementation diets. CHM supplementation decreased (p < 0.05) cracked-egg rate, egg yolk cholesterol, blood serum levels of cholesterol, triglyceride, low-density lipoprotein cholesterol (LDL-C) and alanine aminotransferase (ALT) and increased blood serum levels of high-density lipoprotein cholesterol (HDL-C). No differences in serum total protein, serum albumen, serum glucose and aspartate amino transferase (AST) were observed in hens fed CHM. In conclusion, dietary supplementation with CHM improved egg production and egg quality and reduced serum cholesterol concentrations in laying hens. Dietary supplementation of 1.0% CHM in layer diets may be a feasible means of producing eggs with lower cholesterol and higher egg yolk phospholipid contents for health conscious consumers.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Wang

et al. 2016

Animal Physiology Nutrition

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“…As one of the widely-used traditional medicine, Cedrus deodara displays multiple biological acitivities [ 4 ]. In Indian, the wood of Cedrus deodara has long been used to treat inflammation and rheumatoid arthritis [ 5 , 6 ]. In the Dictionary of Chinese Crude Drugs, Cedrus deodara has been described to display therapeutic potentials in expelling wind, removing dampness, destroying parasites and relieving itches.…”

Section: Introductionmentioning

confidence: 99%

Extraction and purification of total flavonoids from pine needles of Cedrus deodara contribute to anti-tumor in vitro

Shi¹,

Chen²,

Zhang

et al. 2016

BMC Complement Altern Med

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BackgroundCedrus deodara is one of the traditional Chinese medicinal herbs that exhibits a line of biological activities. The current study extracted the total flavonoids from the pine needles of Cedrus deodara (TFPNCD), and investigated its anti-cancer effects in tumor cell lines.MethodsThe total flavonoids was extracted from pine needles of Cedrus deodara by ethanol hot refluxing and purified by HPD722 macroporous resin. The contents of total flavonoids and the active ingredients of TFPNCD were analyzed through UV and HPLC. MTT assay was used to investigate its inhibitory effect on tumor cell lines. The flow cytometry was employed to determine the apoptosis and cell cycle distribution after treated TFPNCD on HepG2 cells.ResultsThe TFPNCD, in which the contents of total flavonoid reached up to 54.28 %, and the major ingredients of myricetin, quercetin, kaempferol and isorhamnetin in TFPNCD were 1.89, 2.01, 2.94 and 1.22 mg/g, respectively. The MTT assays demonstrated that TFPNCD inhibited the growth of HepG2 cells in a dose-dependent manner, with the IC50 values of 114.12 μg/mL. By comparison, TFPNCD inhibited the proliferation to a less extent in human cervical carcinoma HeLa, gastric cancer MKN28 cells, glioma SHG-44 cells and lung carcinoma A549 than HepG2 cells. We found that even at the lower doses, the total flavonoids effectively inhibited the proliferation of HepG2 cells. Comparison of IC50 values implicated that HepG2 cells might be more sensitive to the treatment with total flavonoids. TFPNCD was able to increase the population of HepG2 cells in G0 /G1 phase. Meanwhile, TFPNCD treatment increased the percentage of apoptotic HepG2 cells.ConclusionThese data suggested that TFPNCD might have therapeutic potential in cancer through the regulation of cell cycle and apoptosis.

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“…In addition, reports have also shown that C . deodara possess diverse biological activities such as antibrowning and antimicrobial effects (Zeng and others 2011), and was employed to treat tic, fever, cough, bronchitis, and tuberculosis (Sachin and others 2008; Zhang and others 2009). Despite of these broad uses of pine needle, little information exists as to the exact chemical composition of the essential oils of pine needle ( C .…”

Section: Introductionmentioning

confidence: 99%

Chemical Composition, Antioxidant, and Antimicrobial Activities of Essential Oil from Pine Needle (Cedrus deodara)

Zeng¹,

Zeng²,

Gao³

et al. 2012

Journal of Food Science

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Simultaneous high-performance liquid chromatographic determination of Cedrus deodara active constituents and their pharmacokinetic profile in mice

Cited by 15 publications

References 18 publications

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Effects of Chinese herbal mixture on performance, egg quality and blood biochemical parameters of laying hens

Extraction and purification of total flavonoids from pine needles of Cedrus deodara contribute to anti-tumor in vitro

Chemical Composition, Antioxidant, and Antimicrobial Activities of Essential Oil from Pine Needle (Cedrus deodara)

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