Simultaneous high-performance liquid chromatography determination of three active compounds in snake fruit peel extracts

Abstract

A high-performance liquid chromatography method was developed to simultaneously quantitatively study neochlorogenic acid (Neo), chlorogenic acid (CGA), and procyanidin B4 (B4), which are the bioactive components of snake fruit (Salacca zalacca [Gaertn.] Voss). Snake fruit is an edible plant in the Arecaceae family with oval fruits resembling snake scales. They are frequently grown in Southeast Asia. To achieve separation, the gradient elution mobile phases used acetonitrile and 0.1% trifluoroacetic acid in water on a reversed-phase (C18) analytical column. The detecting wavelength was changed to 279 nm. Accuracy, precision, linearity, and limits of quantification and detection (LOQs and LODs) were all tested following the International Conference on Harmonization (ICH) requirements. Neo, CGA, and B4 were eluted in the proper order under ideal circumstances, and were subjected to chromatogram analysis for 20 min. All bioactive components had linear calibration curves with 0.59–300 µg/mL concentrations. LODs were 0.02–0.13 µg/mL, while LOQs were 0.10–0.44 µg/mL. These three examined chemicals had 83.06%–106.38% recoveries. The precision was <2%, which was within acceptable range. Furthermore, the validated method was performed simply, quickly, and effectively to monitor mixtures of these compounds from 30 extraction processes.