Simultaneous HPLC analysis of crebanine, dicentrine, stephanine and tetrahydropalmatine in Stephania venosa

Kongkiatpaiboon, Sumet; Duangdee, Nongnaphat; Prateeptongkum, Saisuree; Tayana, Ngampuk; Inthakusol, Wichayasith

doi:10.1016/j.bjp.2017.10.004

Cited by 9 publications

(6 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As a result, the Menispermaceae family have been in a focus of rising interest to study several classes of secondary plant metabolites, including alkaloids; which are rather abundant constituents [4][5][6][7][8][9][10][11]. Several classes of alkaloids were isolated from Abuta, those include: isoquinoline [12,13], benzylisoquinoline [12,14], benzyltetraisoquinoline [15], bisbenzyltetraisoquinoline [15][16][17], aporphine [18], and proaporphine [12,15] derivatives; as well as other less frequent ones such as tropolone-isoquinoline, azafluoranthene, and benzazepine alkaloids [19,20].…”

Section: Introductionmentioning

confidence: 99%

Alkaloids of Abuta panurensis Eichler: In silico and in vitro study of acetylcholinesterase inhibition, cytotoxic and immunomodulatory activities

et al. 2020

View full text Add to dashboard Cite

Natural products obtained from species of the genus Abuta (Menispermaceae) are known as ethnobotanicals that are attracting increasing attention due to a wide range of their pharmacological properties. In this study, the alkaloids stepharine and 5-N-methylmaytenine were first isolated from branches of Abuta panurensis Eichler, an endemic species from the Amazonian rainforest. Structure of the compounds was elucidated by a combination of 1D and 2D NMR spectroscopic and MS and HRMS spectrometric techniques. Interaction of the above-mentioned alkaloids with acetylcholinesterase enzyme and interleukins IL-6 and IL-8 was investigated in silico by molecular docking. The molecules under investigation were able to bind effectively with the active sites of the AChE enzyme, IL-6, and IL-8 showing affinity towards the proteins. Along with the theoretical study, acetylcholinesterase enzyme inhibition, cytotoxic, and immunomodulatory activity of the compounds were assessed by in vitro assays. The data obtained in silico corroborate the results of AChE enzyme inhibition, the IC 50 values of 61.24μM for stepharine and 19.55μM for 5-N-methylmaytenine were found. The compounds showed cytotoxic activity against two tumor cell lines (K562 and U937) with IC 50 values ranging from 11.77 μM to 28.48 μM. The in vitro assays revealed that both alkaloids were non-toxic to Vero and human PBMC cells. As for the immunomodulatory activity, both compounds inhibited the production of IL-6 at similar levels. Stepharine inhibited considerably the production of IL-8 in comparison to 5-N-methylmaytenine, which showed a dose dependent action (inhibitory at the IC 50 dose, and stimulatory at the twofold IC 50 one). Such a behavior may possibly be explained by different binding modes of the alkaloids to the interleukin structural fragments. Occurrence of the polyamine alkaloid

show abstract

Section: Introductionmentioning

confidence: 99%

Alkaloids of Abuta panurensis Eichler: In silico and in vitro study of acetylcholinesterase inhibition, cytotoxic and immunomodulatory activities

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Standard XMA was determined by comparing the retention durations and spectra of the sample solution to those of the reference standard. The research was conducted at 25 ˚C and 10 ˚C, or room temperature [10][11][12].…”

Section: Liquid Chromatography Conditionsmentioning

confidence: 99%

Development and validation of HPLC method for ximenynic acid in semisolid dosage formulation

Shivatare¹,

Nagore²,

Jat³

2023

World J. Adv. Res. Rev.

View full text Add to dashboard Cite

A novel analytical technique and validation study were developed to determine the concentration of ximenynic acid (XMA) in semisolid dosage formulations (SDF) such as cream, gel, lotion, etc. The procedure makes use of reverse-phase high-performance liquid chromatography (HPLC). A Phenomenex Luna Column 5 (4.6 x 250 mm) was used for the analysis, using acetonitrile as the mobile phase, sodium dihydrogen phosphate monohydrate as the stationary phase, and 229 nm detection at 30 degrees Celsius. In spite of the presence of additional compounds, their presence had no effect on the detection of XMA in SDF. As part of validating this HPLC technique, a number of tests were performed to evaluate its specificity, linearity, accuracy, precision, and durability. International Conference on Harmonization standards were used to determine whether the method was adequate (ICH). Because it is simple to implement and produces reproducible results, the presented HPLC approach has the potential to be utilized in industry to standardize herbs and Phytomedicines. Because XMA has the potential to be a game-changing method of treating ageing, its advancement may benefit the pharmaceutical and cosmetics sectors.

show abstract

“…The slit dimension was 5×0.45 mm with the scanning speed of 20 mm s-1. The amount of ximenynic acid present in the samples was evaluated by peak area with linear regression [10][11][12].…”

Section: Liquid Chromatography Conditionsmentioning

confidence: 99%

A rapid HPTLC method to estimate ximenynic acid in semisolid dosage formulation

Shivatare¹,

Nagore²,

Jat³

2023

World J. Bio. Pharm. Health Sci.

View full text Add to dashboard Cite

A novel HPTLC analytical technique and validation study were developed to determine the concentration of ximenynic acid (XMA) in semisolid dosage formulations (SDF) such as cream, gel, lotion, etc. The procedure makes use of reverse-phase high-performance liquid chromatography (HPLC). This study presents the first report of sensitive, selective, precise and robust HPTLC method, which has been developed and validated for quantification of the XMA from pharmaceutical formulation. The chromatographic development was carried out on HPTLC plates precoated with silica gel 60 F254 using a mixture of Toluene: Ethyl Acetate: Methanol: Formic acid (5:4:0.5:0.5 v/v/v/v) as mobile phase. Detection was carried out densitometrically at 254 nm. The Rf value of XMA were found at Rf about 0.3 ± 0.01. The method was validated as per ICH guideline with respect to linearity, accuracy, precision, robustness etc. The method is new, simple and economic for routine estimation of XMA in bulk, preformulation studies and pharmaceutical formulation to help the industries as well as researchers for their sensitive determination of XMA rapidly at low cost in routine analysis. Because XMA has the potential to be a game-changing method of treating ageing, its advancement may benefit the pharmaceutical and cosmetics sectors. Ximenynic acid; Skin Care Medicinal Formulation; HPTLC; Validation

show abstract

Simultaneous HPLC analysis of crebanine, dicentrine, stephanine and tetrahydropalmatine in Stephania venosa

Cited by 9 publications

References 19 publications

Alkaloids of Abuta panurensis Eichler: In silico and in vitro study of acetylcholinesterase inhibition, cytotoxic and immunomodulatory activities

Alkaloids of Abuta panurensis Eichler: In silico and in vitro study of acetylcholinesterase inhibition, cytotoxic and immunomodulatory activities

Development and validation of HPLC method for ximenynic acid in semisolid dosage formulation

A rapid HPTLC method to estimate ximenynic acid in semisolid dosage formulation

Contact Info

Product

Resources

About