Simultaneous HPLC-F analysis of three recent antiepileptic drugs in human plasma

Mercolini, Laura; Mandrioli, Roberto; Amore, Mario; Raggi, Maria Augusta

doi:10.1016/j.jpba.2010.02.036

Cited by 35 publications

(18 citation statements)

References 24 publications

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“…Optimal separation, symmetric peaks and shorter retention times were obtained on Eclipse plus C18 column when a mixture of potassium phosphate buffer (0.05 M, pH 5.5) and acetonitrile, instead of methanol, was chosen as a mobile phase. Compared to previously reported HPLC methods [23][24][25][26] our method minimizes the consumption of harmful organic solvents by using lower percentage of organic modifier and lower flow rate of the mobile phase. Additionally, in order to obtain maximal signal intensities of the TPM derivative, its excitation and emission spectra were recorded.…”

Section: Optimization Of the Analytical Methodsmentioning

confidence: 96%

“…5,6,13,14 The availability of a simple, validated and inexpensive analytical method for reliable measurements of drug concentrations in biological fluids is pivotal for its successful utilization in pharmacokinetic and bioequivalence studies, and for therapeutic drug monitoring in various clinical situations. 15 Various analytical methods have been reported for measurement of TPM in biological fluids, including immunoassays, 16,17 gas chromatography (GC) coupled to flame ionization detection (FID) 18 or nitrogen phosphorous detection (NPD), [19][20][21][22] high performance liquid chromatography with UV (HPLC-UV) 23 or fluorescence detection (HPLC-FLD), [24][25][26] capillary electrophoresis with UV detection, 27 liquid chromatography coupled to mass spectrometry (LC-MS), 28,29 and more recently, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). 12,[30][31][32][33][34][35][36] The analysis of TPM in biological fluids is complicated, because like most other carbohydrates and their derivatives, TPM does not contain any chromophores that absorb above 190 nm.…”

Section: Introductionmentioning

confidence: 99%

“…25 Furthermore, HPLC-FLD method using dansyl chloride for derivatization was recently proposed for simultaneous determination of TPM, vigabatrin and gabapentin, with a linearity range of 1-50 μg/mL for TPM. 26 For ionizable analytes capillary electrophoresis could be an alternative to HPLC. Recently, a rapid capillary zone electrophoresis method with indirect UV detection for determination of TPM in human plasma with a linearity range 2-60 μg/mL was developed and validated.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Simple and sensitive high performance liquid chromatography method with fluorescence detection for therapeutic drug monitoring of topiramate

Milosheska¹,

Vovk²,

Grabnar³

et al. 2015

ACSi

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A simple, sensitive, reliable, inexpensive and reproducible HPLC method with fluorescence detection, suitable for routine therapeutic drug monitoring (TDM) of an antiepileptic drug topiramate, was developed and validated. The determination of plasma topiramate concentration was carried out after precolumn derivatization, using 4-chloro-7-nitrobenzofurazan as a fluorescent labeling agent and bendroflumethiazide as an internal standard. The standard calibration curve was linear over the concentration range of 0.01-24 μg/mL (r² > 0.9998). The intra-and inter-day accuracies expressed as bias were from 1.4 to 9.9% and from 1.9 to 10.2%, respectively. The intra-and inter-day precisions were below 7.9% and 2.7%, respectively. The validated method was applied for the measurement of plasma topiramate concentrations in patients with epilepsy. The reported method is appropriate for TDM of topiramate as well as for pharmacokinetic and bioequivalence studies.

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Section: Optimization Of the Analytical Methodsmentioning

confidence: 96%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Simple and sensitive high performance liquid chromatography method with fluorescence detection for therapeutic drug monitoring of topiramate

Milosheska¹,

Vovk²,

Grabnar³

et al. 2015

ACSi

View full text Add to dashboard Cite

show abstract

“…1) The analytical method development for PGB, GBP and VGB determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been proposed [2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] and used for their determination in bulk or pharmaceutical dosage forms, plasma, serum, and urine. These methods employed derivatization reagents such as fluorescamine, [2][3][4][17][18][19] 7-chloro-4-nitrobenzofurazan, [5][6][7] 1-fluoro-2,4-dinitrobenzene, 8) o-phtaldialdehyde, 9,10) 2,4,6-trinitrobenzene sulfonic acid, 11,12) and 9-fluorenylmethylchloroformate.…”

Section: Pregabalin ((3s)-3-(aminomethyl)-5-methylhexanoic Acid) (Pgbmentioning

confidence: 99%

“…2,[5][6][7]17,19) Moreover, HPLC 3,[8][9][10][11][12][13][14]18) and capillary electrophoresis (CE) methods 4) using precolumn derivatization with either UV or fluorescent detector were also used.…”

Section: Pregabalin ((3s)-3-(aminomethyl)-5-methylhexanoic Acid) (Pgbmentioning

confidence: 99%

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

Martinc

Vovk

2013

CHEMICAL & PHARMACEUTICAL BULLETIN

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Pregabalin (PGB), gabapentin (GBP), and vigabatrin (VGB) are structural analogues of γ-aminobutyric acid used for the treatment of different forms of epilepsy. Their analytical determination is challenging since these molecules have no significant UV or visible absorption. Several derivatization methods have been developed and used for their determination in bulk or pharmaceutical dosage forms. We aimed to develop a highthroughput method using a microplate reader with fluorescence detection and simple derivatization with fluorescamine. Obtained method involves derivatization step of only 5 min at room temperature and simultaneous measurements of 96 samples (λ ex 395, λ em 476 nm) thus rendering excellent high-throughput analysis. The method was found to be linear with r 2 >0.998 across investigated analytical ranges of 0.75 to 30.0 µg/ mL for PGB, 2.00 to 80.0 µg/mL for GBP, and 1.50 to 60.0 µg/mL for VGB. Intraday and interday precision values did not exceed 4.93%. The accuracy was ranging between 96.6 to 103.5%. The method was also found to be specific since used excipients did not interfere with the method. The robustness study showed that derivatization procedure is more robust than spectrofluorimetric conditions. The developed high-throughput method was successfully applied for determination of drug content and dissolution profiles in pharmaceutical dosage forms of studied antiepileptic drugs.

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Topiramate: a review of analytical approaches for biological matrices

Pinto

Dolzan

Cabral

et al. 2015

Biomedical Chromatography

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Topiramate is an anticonvulsant drug and it has been used worldwide for a wide range of applications. It is mainly indicated for the treatment of partial and generalized seizures, including Lennox Gastant Syndrome and generalized tonic-clonic seizures, and prophylactic treatment of migraine. Different analytical approaches by high-performance liquid chromatography have been described to analyze topiramate because of its lack of chromophore groups, including derivatization with UV-absorbing moieties, derivatization with fluorescent moieties, refractive index detection, conductivity detection, chemiluminescent nitrogen detection, evaporative light scattering detection and MS detection. In addition, some methods for determination of topiramate by capillary electrophoresis have been published as well as by gas chromatography. Thus, it is beneficial to evaluate and compare these papers before selecting the most suitable method/detector to analyze this drug. This systematic review provides a description of the main analytical methods available for the analysis of topiramate in biological matrices. Each of these methods is briefly discussed considering the detector used with HPLC. HPLC coupled with MS is the main technique used for topiramate analysis in biological matrices, mainly in the electrospray ionization-negative mode.

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Simultaneous HPLC-F analysis of three recent antiepileptic drugs in human plasma

Cited by 35 publications

References 24 publications

Simple and sensitive high performance liquid chromatography method with fluorescence detection for therapeutic drug monitoring of topiramate

Simple and sensitive high performance liquid chromatography method with fluorescence detection for therapeutic drug monitoring of topiramate

A Simple High-Throughput Method for Determination of Antiepileptic Analogues of γ-Aminobutyric Acid in Pharmaceutical Dosage Forms Using Microplate Fluorescence Reader

Topiramate: a review of analytical approaches for biological matrices

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