Simultaneous Hydrolysis of Ellagitannins and Extraction of Ellagic Acid from Defatted Raspberry Seeds Using Natural Deep Eutectic Solvents (NADES)

Teslić, Nikola; Santos, Filipa; Oliveira, Filipe S.; Stupar, Alena; Pojić, Milica; Mandić, Anamarija; Pavlić, Branimir; Cvetanović, Aleksandra; Duarte, Ana Rita C.; Paiva, Alexandre; Mišan, Aleksandra

doi:10.3390/antiox11020254

Cited by 26 publications

(23 citation statements)

References 41 publications

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“…A raspberry seed extract rich in compounds with in vitro anti-cancer activity and in vitro antioxidant activity by extraction with edible, “green” solvent (eutectic mixture consisting of citric acid, water and betaine—a novel and healthier component for food products) was developed. Details about this process have been recently published elsewhere [ 31 ]; however, for better understanding of the entire co-creation concept, some of previously published results are briefly included in the present study. In order to test the idea that produced “green” raspberry seed extract could be used as an ingredient in food products without fear that it has any off-putting properties, in the next phase of research, it was decided to create a soft drink, such as iced tea, in which the extract would serve as a carrier of bioactive components, acidifier, and colorant and to investigate how consumers like the product.…”

Section: Methodsmentioning

confidence: 99%

“…These raspberry seeds are often discarded as waste although they contain a considerable number of bioactive compounds, such as polyphenols, tocopherols, ω-3, and ω-6 fatty acids [ 30 ]. Due to relatively high content of bioactive compounds, high availability, and low market price in Serbia, raspberry seeds were identified as a sustainable alternative source of ellagic acid and other polyphenols with antioxidant and anti-proliferative properties [ 31 ]. Ellagic acid is connected with a broad spectrum of biological effects, such as antioxidant, anti-carcinogenic, anti-obesity, anti-inflammatory and anti-angiogenic, anti-neurodegenerative, and hepatoprotective activity.…”

Section: Introductionmentioning

confidence: 99%

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Communicating Function and Co-Creating Healthy Food: Designing a Functional Food Product Together with Consumers

Vrgović

Pojić

Teslić

et al. 2022

Foods

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Functional food is lately an interesting topic from the new product development perspective; complex motivation and expectations of consumers regarding it present a challenge when new products are designed. Co-creation is an interesting alternative to the standard practices by the R & D departments since it directly involves consumers in the various stages of the creation process. This work aims to describe experiences of engaging consumers in different development stages of a functional food product within a project realized at a food research institute. Four consecutive studies were conducted: the first study explored current trends in Serbia regarding the way consumers use functional food and are informed about it; the second study described development of a raspberry seeds extract with antioxidant and anti-proliferative activity confirmed in vitro; the third study tested the same extract in a sample of consumers, validating its usability in food products; and the fourth study described a co-creation session with 18 participants, during which a number of activities were realized to stimulate idea generation. Rather than the final product idea itself, this work is valuable because of detailed insights into the various phases of the co-creation process. It is shown that consumers and food researchers can together engage in the new food product development process as long as the communication between them is rich and with mutual understanding.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Communicating Function and Co-Creating Healthy Food: Designing a Functional Food Product Together with Consumers

Vrgović

Pojić

Teslić

et al. 2022

Foods

Self Cite

View full text Add to dashboard Cite

show abstract

“…They have also been widely used as antineoplastic drugs or adjuvant drugs [ 38 ]. Their antiproliferative activities can be preliminarily evaluated by the cell viability and selectivity index [ 27 , 39 ]. In this study, we investigated the antiproliferative activity of flavonoid extract against MDA-MB-231, Caco-2, and HepG2 cancer cells with LO-2 cells (normal hepatocytes) as the control ( Figure 6 ).…”

Section: Resultsmentioning

confidence: 99%

Ultrasound-Assisted Natural Deep Eutectic Solvent Extraction and Bioactivities of Flavonoids in Ampelopsis grossedentata Leaves

Zhen

Chen

Geng

et al. 2022

Foods

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We performed ultrasound-assisted extraction coupled with natural deep eutectic solvents (NADES) to achieve the green and efficient preparation of flavonoid extract from Ampelopsis grossedentata leaves. We then evaluated its antioxidant and antiproliferative activities. A NADES consisting of choline chloride and glucose at a molar ratio of 4:1 with 20% water was determined to be the most suitable solvent. The optimal extraction conditions were: a liquid-to-solid ratio of 30 mL/g, an ultrasonication power of 490 W, and an ultrasonication time of 6.5 min. The actual flavonoid yield was 83.93%, which was close to the predicted yield. Further, 86.75% of the flavonoids were recovered by adding the same volume of phosphate buffer saline (100 mM, pH of 7.0) to the extract solution. Although the chemical antioxidant activities of the flavonoid extract were slightly inferior to those of dihydromyricetin, the flavonoid extract could still effectively inhibit the proliferation of human breast MDA-MB-231 cells by inducing cell apoptosis, retarding the cell cycle, changing the mitochondrial membrane potential and scavenging intracellular reactive oxygen species (ROS). The obtained results can provide a reference in the development of plant-derived functional foods.

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“…According to Ekaterina et al [ 38 ], NADES could be efficiently formulated for the recovery of both hydrophilic and lipophilic compounds. The selection and formulation of NADES used in this work was based on applications of NADES extraction for recovery of polyphenols from different plant matrices found in recent literature [ 39 , 40 , 41 , 42 ]. In total, twenty different NADES were selected for the screening study, and they were prepared in a water bath at 80 °C placed on a magnetic stirrer hot plate.…”

Section: Methodsmentioning

confidence: 99%

“…All created NADES were stable at room temperature (≈20 °C) up to more than seven days. Final water content in NADES was mathematically calculated (20 or 25%) and accordingly adjusted for each extraction run according to our previous study [ 39 ]. Initial water content in chemicals and reagents was also taken into consideration for calculation.…”

Section: Methodsmentioning

confidence: 99%

Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts

et al. 2022

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Wild thyme (Thymus serpyllum L.) herbal dust has been recognized as a potential underutilized resource for the recovery of antioxidants. The aim of this paper was to optimize natural deep eutectic solvent (NADES) extraction of polyphenols to obtain improved antioxidant activity of extracts determined by selected in vitro assays (DPPH, FRAP, and ABTS). Twenty different NADES systems were investigated in the first step of the screening of the extraction solvent and l-proline (Pro)–glycerine (Gly) based solvents provided the best results. Preliminary experiments organized by 25−1 fractional factorial design narrowed down the number of extraction factors from five (temperature, extraction time, NADES type, water content and L/S ratio) to three and determined their experimental domain for the final step. A face-centered central composite design with temperature (40–55–70 °C), extraction time (60–120–180 min) and L/S ratio (10–20–30 g NADES/g sample) was applied for influence analysis and process optimization. Multi-response optimization suggested a temperature of 65 °C, time of extraction of 180 min and L/S ratio of 28 g NADES/g DW as optimal extraction parameters. Experimental validation confirmed good agreement between experimental and predicted results in the extract obtained at optimal conditions and the interactions in the most suitable NADES (N16; Pro–Gly–H2O; 1:2:1) were confirmed by the 1H-NMR.

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Simultaneous Hydrolysis of Ellagitannins and Extraction of Ellagic Acid from Defatted Raspberry Seeds Using Natural Deep Eutectic Solvents (NADES)

Cited by 26 publications

References 41 publications

Communicating Function and Co-Creating Healthy Food: Designing a Functional Food Product Together with Consumers

Communicating Function and Co-Creating Healthy Food: Designing a Functional Food Product Together with Consumers

Ultrasound-Assisted Natural Deep Eutectic Solvent Extraction and Bioactivities of Flavonoids in Ampelopsis grossedentata Leaves

Natural Deep Eutectic Solvent (NADES) Extraction Improves Polyphenol Yield and Antioxidant Activity of Wild Thyme (Thymus serpyllum L.) Extracts

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