Simultaneous SIL‐TAL1 RT‐PCR detection of all tald deletions and identification of novel tald variants

Delabesse, Éric; Bernard, Muriel; Landman‐Parker, Judith; Davi, Frédéric; Leboeuf, D; Varet, Bruno; Valensi, Françoise; Macintyre, Elizabeth

doi:10.1046/j.1365-2141.1997.4833286.x

Cited by 36 publications

(46 citation statements)

References 22 publications

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“…189 SIL-TAL1 transcripts are exclusively found in T-ALL. 188,189 The SIL-TAL1 RT-PCR described here detects all SIL-TAL1 deletions other than the single reported case with a TAL1 deletion between exon 4 and exon 5, since the TAL1 primers are located on exon 4 (Figure 19b). It also does not detect TAL1 translocations or 'aberrant' expression of TAL1 without apparent rearrangement.…”

Section: Table 20mentioning

confidence: 75%

Section: Table 20mentioning

confidence: 99%

“…188 Three different SIL-TAL1 transcripts are formed by alternative splicing of the two SIL exons 1a and 1b and TAL1 exons 3 and 4. 188 The predominant transcript (type II) contains SIL exon 1a spliced to TAL1 exons 3 and 4. The two others forms contain SIL exon 1a and exon1b spliced to TAL1 exons 3 and 4 (type III) or SIL exon 1a directly spliced to TAL1 exon 4 (type I) (Figure 19b and Table 21).…”

Section: Table 20mentioning

confidence: 99%

“…The incidence in childhood T-ALL is estimated at approximately 5-25% of cases. 38,39,188 A single study suggested that these genomic deletions are absent in adult T-ALL. 190 SIL-TAL1 transcripts have been found in 26% of childhood T-ALL and 16% of adult T-ALL, predominantly in younger adults.…”

Section: Table 20mentioning

confidence: 99%

“…TAL1 contains seven acceptor deletion sites (taldb1 to taldb7), with two being involved in nearly all cases (taldb1 and taldb2). 38,39,181,[185][186][187][188] In all but one case, these rearrangements create alternative fusion transcripts containing the entire SIL exon 1a and TAL1 exon 4. These transcripts code for a normal TAL1 protein of 42 kDa.…”

Section: Introductionmentioning

confidence: 99%

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1999

Leukemia

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Prospective studies on the detection of minimal residual disease (MRD) in acute leukemia patients have shown that largescale MRD studies are feasible and that clinically relevant MRDbased risk group classification can be achieved and can now be used for designing new treatment protocols. However, multicenter international treatment protocols with MRD-based stratification of treatment need careful standardization and quality control of the MRD techniques. This was the aim of the European BIOMED-1 Concerted Action 'Investigation of minimal residual disease in acute leukemia: international standardization and clinical evaluation' with participants of 14 laboratories in eight European countries (ES, NL, PT, IT, DE, FR, SE and AT). Standardization and quality control was performed for the three main types of MRD techniques, ie flow cytometric immunophenotyping, PCR analysis of antigen receptor genes, and RT-PCR analysis of well-defined chromosomal aberrations. This study focussed on the latter MRD technique. A total of nine well-defined chromosome aberrations with fusion gene transcripts were selected: t(1;19) with E2A-PBX1, t(4;11) with MLL-AF4, t(8;21) with AML1-ETO, t(9;22) with BCR-ABL p190 and BCR-ABL p210, t(12;21) with TEL-AML1, t(15;17) with PML-RARA, inv (16) with CBFB-MYH11, and microdeletion 1p32 with SIL-TAL1. PCR primers were designed according to predefined criteria for single PCR (external primers A ↔ B) and nested PCR (internal primers C ↔ D) as well as for 'shifted' PCR with a primer upstream (E5Ј primer) or downstream (E3Ј primer) of the external A ↔ B primers. The 'shifted' E primers were designed for performing an independent PCR together with one of the internal primers for confirmation (or exclusion) of positive results. Various local RT and PCR protocols were compared and subsequently a common protocol was designed, tested and adapted, resulting in a standardized RT-PCR protocol. After initial testing (with adaptations whenever necessary) and approval by two or three laboratories, the primers were tested by all participating laboratories, using 17 cell lines and patient samples as positive controls. This testing included comparison with local protocols and primers as well as sensitivity testing via dilution experiments. The collaborative efforts resulted in standardized primer sets with a minimal target sensitivity of 10 −2 for virtually all single PCR analyses, whereas the nested PCR analyses generally reached the minimal target sensitivity of 10 −4 . The standardized RT-PCR protocol and primer sets can now be used for molecular classification of acute leukemia at diagnosis and for MRD detection during follow-up to evaluate treatment effectiveness. Keywords: acute myeloid leukemia (AML); acute lymphoblastic leukemia (ALL); PCR; standardization; fusion genes; chromosome PrefaceMonitoring of acute leukemia patients during and after treatment for the presence of remaining leukemic cells ('minimal residual disease', MRD) has been shown to give major insight into the effectiveness of treatment. [1...

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Section: Table 20mentioning

confidence: 75%

Section: Table 20mentioning

confidence: 99%

Section: Table 20mentioning

confidence: 99%

Section: Table 20mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Deregulated expression of theTAL1 gene by t(1;5)(p32;q31) in patient with T-cell acute lymphoblastic leukemia

François¹,

Delabesse

Baranger

et al. 1998

Genes Chromosom. Cancer

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TAL1 gene deregulation is frequent in T‐cell acute lymphoblastic leukemia (T‐ALL) and can result from translocations involving 1p32 or, more frequently, from a cytogenetically undetectable interstitial deletion of chromosome 1. This study presents a case of T‐ALL with a t(1;5)(p32;q31) involving TAL1, in which the breakpoint occurs approximately 10 kbp 5′ to the gene and leads to transcriptional activation and synthesis of a TAL1 protein, and extends the spectrum of recognized TAL1 gene translocations associated with T‐ALL. Genes Chromosomes Cancer 23:36–43, 1998. © 1998 Wiley‐Liss, Inc.

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Acute Lymphoblastic Leukaemia and Acute Leukaemia of Ambiguous Lineage

2017

Leukaemia Diagnosis

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Simultaneous SIL‐TAL1 RT‐PCR detection of all tal^d deletions and identification of novel tal^d variants

Abstract: Summary. Site-specific deletions of the 5 0 part of the TAL1 gene (tal d ) are among the most frequent non-random genetic abnormalities in T-cell acute lymphoblastic leukaemia (T-ALL).

Cited by 36 publications

References 22 publications

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Deregulated expression of theTAL1 gene by t(1;5)(p32;q31) in patient with T-cell acute lymphoblastic leukemia

Acute Lymphoblastic Leukaemia and Acute Leukaemia of Ambiguous Lineage

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