Simultaneous infections by different Salmonellastrains in mesenteric lymph nodes of finishing pigs

Garrido, Victoria; Sánchez, Samanta; Román, Beatriz San; Zabalza-Baranguá, Ana; Díaz-Tendero, Yasmin; Frutos, Cristina de; Jaime, R. C. M.; Grilló, María Jesús

doi:10.1186/1746-6148-10-59

Cited by 26 publications

(25 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Typhimurium strains with different phenotypes (i.e. antimicrobial susceptibility, phage type and/or MLVA patterns) in nine of 14 pigs supported the relative high frequency of this phenomenon of coinfections, as previously reported (Garrido et al., ). Coexistence of pansusceptible and AR Salmonella spp.…”

Section: Discussionsupporting

confidence: 88%

“…The presence of Salmonella spp. in both MLN and IC samples was determined by the well‐standardized ISO 6579:2002/Amd 1:2007 method (hereafter ISO 6579; ISO, ), as recommended in the EU reference studies on pig salmonellosis (EFSA, ) and previously detailed (Garrido et al., ). All the Salmonella isolates were confirmed and classified by serovars according to the Kaufmann–White scheme (Grimont & Weill, ) in the Reference National Centre for Animal Salmonellosis (MAPAMA, Madrid, Spain).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between Salmonella infection, shedding and serology in fattening pigs in low–moderate prevalence areas

Román

Garrido

Sánchez

et al. 2018

Zoonoses and Public Health

View full text Add to dashboard Cite

Salmonella is a major foodborne pathogen causing important zoonosis worldwide. Pigs asymptomatically infected in mesenteric lymph nodes (MLN) can be intermittent shedders of the pathogen through faeces, being considered a major source of human infections. European baseline studies of fattening pig salmonellosis are based on Salmonella detection in MLN. This work studies the relationship between Salmonella infection in MLN and intestinal content (IC) shedding at slaughter and the relationship between the presence of the pathogen and the serologic status at farm level. Mean Salmonella prevalence in the selected pigs (vertically integrated production system of Navarra, Spain) was 7.2% in MLN, 8.4% in IC and 9.6% in serum samples. In this low-moderate prevalence context, poor concordance was found between MLN infection and shedding at slaughter and between bacteriology and serology. In fact, most of shedders were found uninfected in MLN (83%) or carrying different Salmonella strains in MLN and in IC (90%). The most prevalent Salmonellae were Typhimurium resistant to ACSSuT ± Nx or ASSuT antibiotic families, more frequently found invading the MLN (70%) than in IC (33.9%). Multivariable analysis revealed that risk factors associated with the presence of Salmonella in MLN or in IC were different, mainly related either to good hygiene practices or to water and feed control, respectively. Overall, in this prevalence context, detection of Salmonella in MLN is an unreliable predictor of faecal shedding at abattoir, indicating that subclinical infections in fattening pigs MLN could have limited relevance in the IC shedding.

show abstract

Section: Discussionsupporting

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Relationship between Salmonella infection, shedding and serology in fattening pigs in low–moderate prevalence areas

Román

Garrido

Sánchez

et al. 2018

Zoonoses and Public Health

View full text Add to dashboard Cite

show abstract

“…It is worth to mention that a few pigs (5 in trial II and 2 in trial III) shed at some point Salmonella strains different from those isolated from their MLN (Fig. 1), which would support the presence of simultaneous infections by different Salmonella strains in a single pig, as it has been recently described elsewhere (Garrido et al 2014).…”

Section: Discussionsupporting

confidence: 78%

“…), which would support the presence of simultaneous infections by different Salmonella strains in a single pig, as it has been recently described elsewhere (Garrido et al . ).…”

Section: Discussionmentioning

confidence: 97%

Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide

et al. 2014

View full text Add to dashboard Cite

Aims: To assess the efficacy of a b-galactomannan oligosaccharide (ß-GMOS) for the control of Salmonella infection in fattening pigs. Methods and Results: Three different doses (0Á5, 3 and 2 kg ß-GMOS per ton of feed) were used during the entire period of growing in three similar and independent field trials carried out in a small fattening unit (%100 pigs). Treatment was randomly assigned to half of the pens. Individual serum samples (20-25 per group) were collected at different times during the fattening period and a similar number of faecal samples during the fattening period and at slaughter. In addition, mesenteric lymph nodes were collected at slaughter. Herdcheck â Swine Salmonella ELISA was used for serological analyses, the ISO 6579:2002/Amd 1 : 2007 for bacteriology and the PFGE for molecular characterization of Salmonella strains. The addition of ≥2 kg t À1 of ß-GMOS to the pig diet during the entire fattening period was associated with a reduction in Salmonella prevalence, shedding and seroconversion. Conclusions: Feed supplementation with ß-GMOS may be a useful complementary tool for the control of salmonellosis in fattening pigs. Significance and Impact of the Study: ß-GMOS may be a complementary way of reducing Salmonella shedding and infection in fattening pigs.

show abstract

“…), supporting the occurrence of new infections. Pigs within this group that showed different serotypes in MLN and SFEC samples (36%) may have actually suffered simultaneous infections by different Salmonella strains, as previously reported in pigs (Garrido et al., ).…”

Section: Discussionsupporting

confidence: 62%

Influence of On‐farm pig Salmonella status on Salmonella Shedding at Slaughter

Casanova-Higes

Andrés-Barranco

Jaime

2016

Zoonoses and Public Health

View full text Add to dashboard Cite

The risk of Salmonella shedding among pigs at slaughter with regard to their previous on-farm Salmonella status was assessed in a group of pigs from a farm from NE of Spain. A total of 202 pigs that had been serologically monitored monthly during the fattening period and from which mesenteric lymph nodes (MLN) and faecal (SFEC) samples were collected at slaughter for Salmonella isolation were included. A repeated-measures anova was used to assess the relationship between mean OD% values during the fattening period and sampling time and bacteriology on MLN and SFEC. Pigs were also grouped into four groups, that is pigs seronegative during the fattening period and Salmonella negative in MLN (group A; n = 69); pigs seronegative during the fattening period but Salmonella positive in MLN (B; n = 36); pigs seropositive at least once and Salmonella positive in MLN (C; n = 50); and pigs seropositive at least once but Salmonella negative in (D; n = 47). Pigs shedding at slaughter seroconverted much earlier and showed much higher mean OD% values than non-shedders pigs. The proportion of Salmonella shedders in groups A and D was high and similar (26.1% and 29.8%, respectively), but significantly lower than that for groups B and C. The odds of shedding Salmonella for groups B and C were 4.8 (95% CI = 1.5-15.5) and 20.9 (3.7-118) times higher, respectively, when compared to A. It was concluded that a large proportion of Salmonella seronegative pigs may shed Salmonella at slaughter, which would be likely associated to previous exposure with contaminated environments (i.e. transport and lairage). For pigs already infected at farm, the likelihood of shedding Salmonella was much higher and may depend on whether the bacterium has colonized the MLN or not. The odds of shedding Salmonella spp. were always much higher for pigs in which Salmonella was isolated from MLN.

show abstract

Simultaneous infections by different Salmonellastrains in mesenteric lymph nodes of finishing pigs

Cited by 26 publications

References 21 publications

Relationship between Salmonella infection, shedding and serology in fattening pigs in low–moderate prevalence areas

Relationship between Salmonella infection, shedding and serology in fattening pigs in low–moderate prevalence areas

Reduction of subclinical Salmonella infection in fattening pigs after dietary supplementation with a ß-galactomannan oligosaccharide

Influence of On‐farm pig Salmonella status on Salmonella Shedding at Slaughter

Contact Info

Product

Resources

About