Simultaneous injection effective mixing flow analysis of urinary albumin using dye-binding reaction

Ratanawimarnwong, Nuanlaor; Ponhong, Kraingkrai; Teshima, Norio; Nacapricha, Duangjai; Grudpan, Kate; Sakai, Tadao; Motomizu, Shoji

doi:10.1016/j.talanta.2012.02.027

Cited by 16 publications

(13 citation statements)

References 23 publications

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“…air-carrier continuous analysis system [22], sequential injection analysis [23], multi-commutation in flow analysis [24], multisyringe flow injection analysis [25], all injection analysis [26], multi-pumping in flow analysis [27], sequential injection chromatography [28] and sequential injection lab at valve [29]. Our laboratory also has conceived new flow-based analysis methods, such as stopped-in-loop flow analysis (SILFA) [30] and simultaneous injection effective mixing flow analysis (SIEMA) [31,32]. These techniques are capable of lower reagent(s) consumption and lower waste generation compared with a conventional FIA technique.…”

Section: Introductionmentioning

confidence: 99%

Stopped-in-loop flow analysis system for successive determination of trace vanadium and iron in drinking water using their catalytic reactions☆

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Ohara

Ratanawimarnwong

et al. 2015

Talanta

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Section: Introductionmentioning

confidence: 99%

Stopped-in-loop flow analysis system for successive determination of trace vanadium and iron in drinking water using their catalytic reactions☆

Quezada

Ohara

Ratanawimarnwong

et al. 2015

Talanta

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“…3a, NaOH concentration of 0.5% reached a maximum absorbance of the creatinine-picric acid complex. The absorbance decreased along with the increasing NaOH concentration due to the formation of sodium picric precipitate under high alkaline condition 4 . Therefore, NaOH concentration of 0.5% was selected for creatinine determination throughout the experiment.…”

Section: Fig 2 Effects Of Ph Of Methyl Orange/mo (A) and Volume Of mentioning

confidence: 99%

“…Albumin and creatinine is an important indicator for albuminuria and widely known as an early marker of diabetes, kidney disease, and hypertension. [4][5][6] Detection of microalbuminuria is imperative to identify individuals at high risk of kidney disease. One of the methods commonly used to detect microalbuminuria and has been recommended by the National Kidney Foundation is albumin-creatinine ratio (ACR) measurement in a random urine specimen.…”

Section: Introductionmentioning

confidence: 99%

Sequential Injection at Valve Mixing (SI-VM) for Determination of Albumin-Creatinine Ratio in Urine

Sabarudin¹

2018

Orient. J. Chem

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Measurement of the albumin-creatinine ratio (ACR) as a medical index of renal failure is imperative to identify individuals at high risk of kidney disease. The present study is aimed to develop albumin and creatinine measurement method by sequential injection at valve mixing (SI-VM) for the determination of urinary ACR. The laboratory-made SI-VM system consists of a syringe pump, 8-port selection valve, RGB-LED detector, and holding coil. This system was controlled by a computer using a home-made software prepared by the Visual Basic program. The detection of albumin was based on dye-binding of methyl orange, while the Jaffe reaction was applied for the creatinine detection. Both albumin and creatinine were simultaneously and automatically measured using SI-VM, and the absorbance was recorded at 530 nm. Some parameters affected sensitivity, precision, and accuracy of the method, which includes pH of methyl orange, the volume of albumin, the concentration of NaOH, NaOH-picric acid ratio, and flow rate to detector were studied in detail. The proposed method was successfully applied to the determination of ACR in urine samples with satisfied results. Additionally, a rapid, sensitive, and accurate determination of ACR could be attributed to the SI-VM method.

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“…[9][10][11] Less reagents requirement accompanied by a discrete volume of solution and easy handling could be attributed to this SIA system. [12][13][14] However, the formation of the segments in a holding coil resulted in imperfect mixing between reagents and sample. To solve this problem, a mixing chamber/tip can be installed into a selection valve of the SIA system.…”

Section: Introductionmentioning

confidence: 99%

DETERMINATION OF Zn(II) IN DRINKING WATER USING SEQUENTIAL INJECTION-VALVE MIXING WITH ALIZARIN RED S AS COMPLEXING AGENT

Sabarudin¹

2018

RJC

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The typical method for determination of Zn(II) in drinking water is atomic absorption spectrophotometry. However, this method requires a large amount of sample solution, reagent (nitric acid), and also time-consuming. Therefore, in this study, Sequential Injection-Valve Mixing (SI-VM) with RGB Detector at 520 nm by employing Alizarin Red S (ARS) as the complexing agent was developed for the determination of Zn(II). The optimum conditions for the complex formation of Zn-ARS are as follows: pH 4.5, ARS concentration of 5 x10 -5 M, ARS volume of 150 µL, a sample volume of 50 µL, the reaction time of 8s, and flow rate of the reaction products toward detector is 50 µL/s. This method is successfully applied to the determination of Zn(II) in drinking water with a detection limit of 5.9 µg/L (ppb). High sensitivity, less reagent and sample consumptions (200 µL), and high sample throughput (100 sample/h) can be attributed to the advantages of the proposed method.

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Simultaneous injection effective mixing flow analysis of urinary albumin using dye-binding reaction

Cited by 16 publications

References 23 publications

Stopped-in-loop flow analysis system for successive determination of trace vanadium and iron in drinking water using their catalytic reactions☆

Stopped-in-loop flow analysis system for successive determination of trace vanadium and iron in drinking water using their catalytic reactions☆

Sequential Injection at Valve Mixing (SI-VM) for Determination of Albumin-Creatinine Ratio in Urine

DETERMINATION OF Zn(II) IN DRINKING WATER USING SEQUENTIAL INJECTION-VALVE MIXING WITH ALIZARIN RED S AS COMPLEXING AGENT

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