2020
DOI: 10.1039/d0nr04718j
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Simultaneous introduction of multiple biomacromolecules into plant cells using a cell-penetrating peptide nanocarrier

Abstract: Simple and efficient cell-penetrating peptide-based multiple DNA, RNA and protein codelivery platforms to plant cells have been developed.

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Cited by 27 publications
(23 citation statements)
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“…The direct introduction of proteins for trait addition to plants has been studied as an alternative method to gene recombination. , One of these methods is conjugation of a target protein with cell-penetrating peptides (CPPs). CPPs are useful tools for efficiently transporting substances such as nucleic acids and proteins into cells. Various CPPs have already been developed and are expected to be applied to the medical field. We have succeeded in introducing proteins such as Citrine, bovine serum albumin, and green fluorescent protein into plant cells by complexing them with CPPs via ionic interactions. , This technique enabled us to impart transient kanamycin resistance to apple leaves by introducing a complex of CPP-fused peptide and neomycin phosphotransferase II (NPTII), a kanamycin resistance protein.…”
Section: Introductionmentioning
confidence: 99%
“…The direct introduction of proteins for trait addition to plants has been studied as an alternative method to gene recombination. , One of these methods is conjugation of a target protein with cell-penetrating peptides (CPPs). CPPs are useful tools for efficiently transporting substances such as nucleic acids and proteins into cells. Various CPPs have already been developed and are expected to be applied to the medical field. We have succeeded in introducing proteins such as Citrine, bovine serum albumin, and green fluorescent protein into plant cells by complexing them with CPPs via ionic interactions. , This technique enabled us to impart transient kanamycin resistance to apple leaves by introducing a complex of CPP-fused peptide and neomycin phosphotransferase II (NPTII), a kanamycin resistance protein.…”
Section: Introductionmentioning
confidence: 99%
“…One possible improvement of recombination efficiency might be achieved with new CPPs such as BPCH7, which efficiently releases DNA after penetrating inside the cell ( Chuah and Numata, 2018 ); the block copolymer maleimide-conjugated tetra(ethylene glycol) and poly( l -lysine; MAL-TEG-PLL; Miyamoto et al, 2019 ), an endosome-escaping micelle ( Miyamoto et al, 2021 ); and new candidates from a list of 55 peptides ( Numata et al, 2018 ) for efficient gene delivery to chloroplasts. These CPPs may be included into the current cpPD complex as conjugates ( Thagun et al, 2019 , 2020 ), which have raised the transformation efficiency of various types of plastids. Furthermore, based on the behavior of the cpPD complex in the present study, future targets to improve recombination efficiency include changing the properties of the cell wall, harnessing uptake mechanisms such as phagocytosis or pinocytosis, and increasing the colocalization of cp-nucleoids and plasmid DNA.…”
Section: Discussionmentioning
confidence: 99%
“… 17 In addition, functional proteins were successfully internalized into plant cells via functionalized CPPs. 18 , 24 …”
Section: Introductionmentioning
confidence: 99%
“… 25 , 27 The use of these functionalized CPPs and OTPs highlights the advanced development of peptide-based biomolecule delivery systems for plants. Various combinations of cationic CPPs and OTPs have been successfully used to deliver plasmid DNA harboring reporter gene expression cassettes to nuclei and targeted organelles in plant cells using syringe infiltration, 16 18 , 24 27 vacuum/compression infiltration, 26 , 28 or injection. 27 However, these techniques are impractical for reprogramming plant quality traits under agricultural conditions.…”
Section: Introductionmentioning
confidence: 99%