Simultaneous isolation of human milk ribonuclease and lysozyme

Indigenous enzymes in milk: Overview and historical aspects—Part 2

“…Chandan, Parry, and Shahani (1968) reported that bovine milk contains about three times as much RNase as human, ovine or caprine milk and that porcine milk contains a very low level of RNase. The same group (Dalaly, Eitenmiller, Vakil, & Shahani, 1970) purified RNase from human milk; the principal isozyme contained no carbohydrate but the minor one was a glycoprotein. The enzyme hydrolysed RNA, polycytidylic and polyuridylic acids, but not polyadenylic or polyguanylic acids or DNA.…”

“…The enzyme hydrolysed RNA, polycytidylic and polyuridylic acids, but not polyadenylic or polyguanylic acids or DNA. Dalaly et al (1970) considered milk RNase to be generally similar to bovine pancreatic RNase. Further characterisation of the two human milk isozymes was reported by Dalaly, Eitenmiller, Friend, and Shahani (1980).…”

“…Variability in the level of lysozyme in human milk and its heat stability were studied by Chandan, Shahani, and Holly (1964) and the isolation procedure was improved by Jolles and Jolles (1967) and Parry, Chandan, and Shahani (1969); a method for the simultaneous isolation of RNase and lysozyme from human milk was reported by Dalaly et al (1970).…”

“…Bovine milk lysozyme (BML) was isolated and characterised by Chandan et al (1965), Dalaly et al (1970), Shahani (1971, 1976), and Eitenmiller, Friend, Shahani, and Ball (1974). Equine milk lysozyme was isolated and characterised by Jauregui-Adell (1971 and Jauregui-Adell, Cladel, Ferraz-Pina, and Rech, (1972).…”