Simultaneous LC-MS/MS bioanalysis of alkaloids, terpenoids, and flavonoids in rat plasma through salting-out-assisted liquid-liquid extraction after oral administration of extract from Tetradium ruticarpum and Glycyrrhiza uralensis: a sample preparation strategy to broaden analyte coverage of herbal medicines

Li, Manlin; Wang, Hanxue; Huan, Xiaohan; Cao, Ning; Guan, Huida; Zhang, Hongmei; Cheng, Xuemei; Wang, Changhong

doi:10.1007/s00216-021-03568-1

Cited by 21 publications

(12 citation statements)

References 37 publications

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“…The most generic method to prepare plasma and serum samples in large scale studies is protein precipitation (PPT), by adding 3–5 times the sample’s volume of organic solvents such as acetonitrile, methanol, ethanol or mixtures of the aforementioned, and/or acidified solutions depending on the stability of analytes in low pH [ 35 ]. Even though PPT is considered a rapid and simple method, it is a non-selective technique offering poor sample cleanup, as major endogenous substances such as phospholipids tend to coelute with the analytes, leading to interferences during chromatographic separation and ionization in the mass spectrometer [ 36 , 37 ]. In the present study, both plasma and serum samples that underwent the PPT methods had the lowest % recoveries; the majority of the studied analytes attained % recoveries ranging around 40%.…”

Section: Resultsmentioning

confidence: 99%

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

et al. 2022

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Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food.

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Section: Resultsmentioning

confidence: 99%

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

et al. 2022

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“…n ‐Butanol was used to extract large polar compounds, so most compounds in the samples could be extracted using it and ethyl acetate. Manlin Li et al evaluated six sample pretreatment methods using LC–MS/MS, suggesting that extraction using LLE with ethyl acetate– n ‐butanol yielded high matrix effect values (M. L. Li et al, 2021). This method was simple and convenient compared with other methods and can minimize the effect of ion suppression, especially suitable for the quantitative analysis of ginsenosides.…”

Section: Resultsmentioning

confidence: 99%

The antitumor activity and pharmacokinetics research of PPD‐Arg (Tos) using ultra‐performance liquid chromatography‐quadrupole‐time‐of‐flight mass spectrometry

Liu

et al. 2022

Biomedical Chromatography

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In this study, a new compound PPD‐Arg (Tos) (PAT), an arginine derivative of 20(s)‐PPD, was synthesized via Fmoc‐Arg (Tos)‐OH and 20(s)‐PPD. The pharmacokinetic properties in rats, in vitro cytotoxicity, and cell apoptosis rates of protopanaxadiol (PPD) and PAT were determined. A sensitive bioanalytical method for pharmacokinetics using ultra‐performance liquid chromatography coupled with time‐of‐flight mass spectrometry was developed and validated. The result showed that the Tmax and t1/2 of PAT were significantly enhanced, indicating a long‐lasting effect in vivo. Compared to the PPD group, the PAT group showed higher bioavailability. PAT also exhibited higher antitumor efficacy than PPD against three cancer cells, especially the strongest inhibitory activity against Huh‐7, even more potent than the positive control of paclitaxel. Therefore, the apoptosis assay based on annexin V/propidium iodide–combined staining against Huh‐7 further demonstrated that PAT could induce apoptosis of Huh‐7 cells. Better pharmacokinetic properties and antitumor efficacy of the arginine derivative of 20(s)‐PPD were important. These findings could provide references for further clinical research on amino acid derivatives of PPD as antitumor agents.

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“…It is necessary to consider the improvement of the bioavailability in subsequent formulation designs. However, compared with limonin, the time for HY-071085 to reach the maximum plasma concentration in rat and beagle dog plasma was significantly shortened [ 24 , 28 , 29 , 32 ]. This may be related to the increase of the distribution coefficient (log D ) and partition coefficient (log P ) values of HY-071085 ( Table S6 ) [ 10 ].…”

Section: Discussionmentioning

confidence: 99%

Preclinical Drug Pharmacokinetic, Tissue Distribution and Excretion Profiles of the Novel Limonin Derivate HY-071085 as an Anti-Inflammatory and Analgesic Candidate in Rats and Beagle Dogs

et al. 2022

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Limonin is one of the research hotspots in natural drug development. However, its low solubility in water leads to poor oral bioavailability, discouraging the further study of its potential as a candidate compound. In order to overcome this limitation, and to enhance its biological activities, a novel limonin derivative—HY-071085—was synthesized by structural modification, and has exhibited strong anti-inflammatory and analgesic activity. In order to achieve a thorough understanding of the biological actions of HY-071085 in vivo, this study evaluated the pharmacokinetics and bioavailability of HY-071085 in rats and beagle dogs, and the distribution and excretion in rats. Using ultra-high-performance liquid chromatography-tandem mass spectrometry, the kinetic profiles of HY-071085 in the plasma of healthy rats and beagle dogs after a single gavage, repeated gavages and the intravenous injection of HY-071085 were studied. The tissue distribution (heart, liver, spleen, lung, kidney, gastric tissue, intestine, brain, skin, testis, ovary and womb) and excretion of HY-071085 were also studied. These results showed that HY-071085 has nonlinear dynamic characteristics in rat and beagle dog plasma. It was found that the plasma concentrations of HY-071085 in female rats were significantly higher than those in male rats after a single oral administration. There were gender differences in the kinetic behavior of HY-071085 in rats; however, there was no difference identified in dogs. HY-071085 was mainly eliminated as metabolites in rats, and was distributed in most of the tissues except the brain, with the highest content being in the gastric tissue and intestinal arease, followed by the liver, spleen, fat, lung, kidney, ovary and heart. The bioavailability of HY-071085 in male and female rats was 2.8% and 10.8%, respectively, and was about 13.1% in beagle dogs. The plasma protein binding rate of HY-071085 in rats, beagle dogs and humans ranged from 32.9% to 100%, with obvious species differences. In conclusion, our study provides useful information regarding the absorption, distribution and excretion of HY-071085, which will provide a good base for the study of the mechanism of its biological effects.

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Cited by 21 publications

References 37 publications

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

The antitumor activity and pharmacokinetics research of PPD‐Arg (Tos) using ultra‐performance liquid chromatography‐quadrupole‐time‐of‐flight mass spectrometry

Preclinical Drug Pharmacokinetic, Tissue Distribution and Excretion Profiles of the Novel Limonin Derivate HY-071085 as an Anti-Inflammatory and Analgesic Candidate in Rats and Beagle Dogs

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