Simultaneous Measurement of 11 Antibiotics for Use in the Intensive Care Unit by Ultra-High Performance Liquid Chromatography–Tandem Mass Spectrometry

Abstract

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Expert consensus statement on therapeutic drug monitoring and individualization of linezolid

“…The quality control of linezolid by in vivo drug analysis techniques should include investigations of specificity, sensitivity, accuracy, reproducibility, and stability ( 22 – 27 ). Quality control standards should include indicators for quantitative determination methods within and between laboratories, qualifications and certification of testing personnel, Standard Operating Procedure (SOPs) for testing procedures, clinical pathways and independent external quality assessments (EQAs) ( 31 ).…”

“…HPLC-based methods coupled with UV or MS are the major analytical techniques for determining linezolid in human plasma or serum, and MS is more suitable for clinical application because of its high selectivity and sensitivity ( 21 ). LC-MS/MS methods that enable simultaneous quantification of plasma concentrations of linezolid and other antibiotics are available ( 22 – 27 ) and may improve detection efficiency for TDM. Other methods for determining linezolid in human plasma are immunoassay ( 28 ), direct analysis in real time mass spectrometry (DART-MS) ( 29 ), and square wave voltammetry ( 30 ), but these lack comparative verification with HPLC for large-scale clinical sample detection.…”