Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC–ESI-MS/MS

Cannazza, Giuseppe; Carrozzo, Marina M.; Cazzato, Addolorata Stefania; Bretis, Irina M.; Troisi, Luigino; Parenti, Carlo; Braghiroli, Daniela; Guiducci, Stefania; Zoli, Michèle

doi:10.1016/j.jpba.2012.08.004

Cited by 41 publications

(25 citation statements)

References 10 publications

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“…We would like to state that while AcbC is a good target for sampling DA it might not be a good option for NE, EP, 5-HT or AD sampling. However, these neurochemicals have been collected and measured from AcbC as previously reported (Salem and Hope, 1999;Del Arco et al, 2008;Hernandez et al, 2008;Rada et al, 2010;Cannazza et al, 2012;Dobbs and Mark, 2012;Munari et al, 2013;Pajski and Venton, 2013;Dautan et al, 2014). Thus, these evidences indicate the trustworthy of our study.…”

Section: Discussionsupporting

confidence: 77%

Revealing the role of the endocannabinoid system modulators, SR141716A, URB597 and VDM-11, in sleep homeostasis

et al. 2016

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Abstract-The endocannabinoid system comprises receptors (CB 1 and CB 2 cannabinoid receptors), enzymes (Fatty Acid Amide Hydrolase [FAAH], which synthesizes the endocannabinoid anandamide), as well as the anandamide membrane transporter (AMT). Importantly, previous experiments have demonstrated that the endocannabinoid system modulates multiple neurobiological functions, including sleep. For instance, SR141716A (the CB 1 cannabinoid receptor antagonist) as well as URB597 (the FAAH inhibitor) increase waking in rats whereas VDM-11 (the blocker of the AMT) enhances sleep in rodents. However, no further evidence is available regarding the neurobiological role of the endocannabinoid system in the homeostatic control of sleep. Therefore, the aim of the current experiment was to test if SR141716A, URB597 or VDM-11 would modulate the sleep rebound after sleep deprivation. Thus, these compounds were systemically injected (5, 10, 20 mg/kg; ip; separately each one) into rats after prolonged waking. We found that SR141716A and URB597 blocked in dose-dependent fashion the sleep rebound whereas animals treated with VDM-11 displayed sleep rebound during the recovery period. Complementary, injection after sleep deprivation of either SR141716A or URB597 enhanced dose-dependently the extracellular levels of dopamine (DA), norepinephrine (NE), epinephrine (EP), serotonin (5-HT), as well as adenosine (AD) while VDM-11 caused a decline in contents of these molecules. These findings suggest that SR141716A or URB597 behave as a potent stimulants since they suppressed the sleep recovery period after prolonged waking. It can be concluded that elements of the endocannabinoid system, such as the CB 1 cannabinoid receptor, FAAH and AMT, modulate the sleep homeostasis after prolonged waking. Ó

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Section: Discussionsupporting

confidence: 77%

Revealing the role of the endocannabinoid system modulators, SR141716A, URB597 and VDM-11, in sleep homeostasis

et al. 2016

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“…Moriarty et al provide a LC-ESI-MS/MS method for the analysis of SRT and related compounds in urine and achieved LODs of 8.8–18.2 nM and LOQs of 29.4 – 55.7 nM [42]. Only a few publications measuring neurotransmitters in brain microdialysates exist, including Suominen et al presenting LODs for DA and SRT analyzed by LC-ESI-MS/MS of 0.20 nM (3 fmol injected onto the column) and an LOQ of 0.5 nM for DA and 1 nM for SRT [16, 17, 43]. Due to the high sensitivity of the developed method (LOQ DA: 0.37 nM; LOQ SRT: 0.14 nM; summarized in Table 2), the number of requisite worms (L1 stage) could be reduced to about 2,000 per sample compared to other HPLC methods requiring hundreds of thousands worms (200,000 worms) per sample [34, 44, 45].…”

Section: Discussionmentioning

confidence: 99%

Highly sensitive isotope-dilution liquid-chromatography–electrospray ionization–tandem-mass spectrometry approach to study the drug-mediated modulation of dopamine and serotonin levels in Caenorhabditis elegans

Schumacher

Chakraborty

Kleuser

et al. 2015

Talanta

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Dopamine (DA) and serotonin (SRT) are monoamine neurotransmitters that play a key role in regulating the central and peripheral nervous system. Their impaired metabolism has been implicated in several neurological disorders, such as Parkinson’s disease and depression. Consequently, it is imperative to monitor changes in levels of these low-abundant neurotransmitters and their role in mediating disease. For the first time, a rapid, specific and sensitive isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for the quantification of DA and SRT in the nematode Caenorhabditis elegans (C. elegans). This model organism offers a unique approach for studying the effect of various drugs and environmental conditions on neurotransmitter levels, given by the conserved DA and SRT biology, including synaptic release, trafficking and formation. We introduce a novel sample preparation protocol incorporating the usage of sodium thiosulfate in perchloric acid as extraction medium that assures high recovery of the relatively unstable neurotransmitters monitored. Moreover, the use of both deuterated internal standards and the multiple reaction monitoring (MRM) technique allows for unequivocal quantification. Thereby, to the best of our knowledge, we achieve a detection sensitivity that clearly exceeds those of published DA and SRT quantification methods in various matrices. We are the first to show that exposure of C. elegans to the monoamine oxidase B (MAO-B) inhibitor selegiline or the catechol-O-methyltransferase (COMT) inhibitor tolcapone, in order to block DA and SRT degradation, resulted in accumulation of the respective neurotransmitter. Assessment of a behavioral output of the dopaminergic system (basal slowing response) corroborated the analytical LC-MS/MS data. Thus, utilization of the C. elegans model system in conjunction with our analytical method is well-suited to investigate drug-mediated modulation of the DA and SRT system in order to identify compounds with neuroprotective or regenerative properties.

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“…, and tph2 −/− embryos at E10.5 were obtained and used for the analysis as previously described (Carrera et al, 2007;Cannazza et al, 2012). The standard substance of 5-HT was purchased from Sigma-Aldrich.…”

Section: Lc-ms Analysismentioning

confidence: 99%

5-hydroxytryptamine synthesized in the aorta-gonad-mesonephros regulates hematopoietic stem and progenitor cell survival

Wang

Liu

2017

Mechanisms of Development

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Simultaneous measurement of adenosine, dopamine, acetylcholine and 5-hydroxytryptamine in cerebral mice microdialysis samples by LC–ESI-MS/MS

Cited by 41 publications

References 10 publications

Revealing the role of the endocannabinoid system modulators, SR141716A, URB597 and VDM-11, in sleep homeostasis

Revealing the role of the endocannabinoid system modulators, SR141716A, URB597 and VDM-11, in sleep homeostasis

Highly sensitive isotope-dilution liquid-chromatography–electrospray ionization–tandem-mass spectrometry approach to study the drug-mediated modulation of dopamine and serotonin levels in Caenorhabditis elegans

5-hydroxytryptamine synthesized in the aorta-gonad-mesonephros regulates hematopoietic stem and progenitor cell survival

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