Simultaneous Measurement of C14 and H3 during Gas-Liquid Chromatography

Popják, G.; Löwe, Arno; Moore, D.

doi:10.1007/978-1-4684-8619-3_24

Cited by 5 publications

(6 citation statements)

References 9 publications

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“…The acidic products of the degradation were methylated and examined by the gas-liquid radiochromatographic technique of Popjak, Lowe & Moore (1962). Figure 7 shows the record of the gas-liquid chromatographic analysis.…”

Section: -2 (B)mentioning

confidence: 99%

“…The alcohol was analysed by the gas-liquid radiochromatographic technique of Popjak, Lowe & Moore (1962) on an ethylene glycol-succinate polyester column a t 100 °C: 88-6 % of the radioactivity in it was associated with 3,3-dimethylallyl alcohol and the remainder (1*8 and 9*6% respectively) with two unidentified substances having retention times of 0-25 and 0-45 relative to th at of the dimethylallyl alcohol.…”

Section: Degradation Ofmentioning

confidence: 99%

“…The clear ether solution was evaporated to dryness, the residue dissolved in 50 /A. of benzene, and analysed by gas-liquid radiochromatography (Popjak, Lowe & Moore 1962). The column used was 6 ft. long, 4 mm diameter, packed with Celite (80 to 120 mesh) coated with 15% (w/w) of ethylene glycol-succinate polyester.…”

Section: Degradation Of 'A '-Tritio N a D H To Succinic Acidmentioning

confidence: 99%

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Studies on the biosynthesis of cholesterol. XVII. The asymmetric synthesis of a symmetrical molecule

Cornforth

Donninger

et al. 1966

Proc. R. Soc. Lond. B.

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The differentiation between two identical chemical groups in the Ca'a"bd molecule in enzymic reactions is discussed first. It is then shown that squalene biosynthesized from two molecules of [1-D 2 ]farnesyl pyrophosphate is asymmetrically labelled at one of its central methylene carbon atoms, one of the deuterium atoms at C-l of one of the two farnesyl residues having been replaced stereospecifically by H B of reduced nicotinamide adenine dinucleotide phosphate. The trideuterio succinic acid obtained by ozonolysis from such squalene is shown to be dextrorotatory and to have the S absolute configuration. The steric position of H A and of H B at position 4 of the dihydronicotinamide ring in NADH and NADPH, transferred from substrates to coenzyme by ‘A’- and ‘B’-specific dehydrogenases respectively, is deduced. This was achieved by making a correlation between the laevorotatory 2 R -[2-D 1 ]succinic acid (made chemically from enzymically generated 2 S -3 R -[3-D 1 ]malic acid) and the [2-D 1 ]succinic acids derived by chemical degradation from positions 3, 4, 5 and 6 of the dihydronicotinamide ring of ‘A’-deuterio and ‘B’-deuterio NADH. The absolute configuration of C-4 of the dihydronicotinamide ring in ‘A’-deuterio NADH is R and in the ‘B’-deuterio NADH it is S .

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Section: -2 (B)mentioning

confidence: 99%

Section: Degradation Ofmentioning

confidence: 99%

Section: Degradation Of 'A '-Tritio N a D H To Succinic Acidmentioning

confidence: 99%

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Studies on the biosynthesis of cholesterol. XVII. The asymmetric synthesis of a symmetrical molecule

Cornforth

Donninger

et al. 1966

Proc. R. Soc. Lond. B.

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“…All the gas-liquid chromatography, analytical and preparative, was done on columns packed with Celite (100-120 mesh) coated with 10% Carbowax 20M (Union Carbide Corp., New York, N.Y., U.S.A.). In the analytical instrument, coupled with a radioactivity detector (Popjak, Lowe & Moore, 1962), a 9 ft. long x 4mm. diam.…”

Section: Methodsmentioning

confidence: 99%

Synthesis of 10,11-dihydrofarnesyl pyrophosphate from 6,7-dihydrogeranyl pyrophosphate by prenyltransferase

Popják

Holloway

Baron

1969

Biochemical Journal

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The syntheses of 6,7-dihydrogeraniol and of its pyrophosphate are described. It is shown that this analogue of geranyl pyrophosphate is a substrate for liver prenyltransferase and that the product synthesized by this enzyme from it and isopentenyl pyrophosphate is 10,11-dihydrofarnesyl pyrophosphate. The K(m) value for 6,7-dihydrogeranyl pyrophosphate was determined to be 1.11+/-0.19mum as compared with 4.34+/-1.71mum for geranyl pyrophosphate. The maximum reaction velocity with the artifical substrate was, however, only about one-fourth of that observed with geranyl pyrophosphate. The binding of isopentenyl pyrophosphate to the enzyme was not affected by the artificial substrate.

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“…x 4mm.) used in gas-liquid radiochromatography (Popjak, Lowe & Moore, 1962) was packed with Celite (100-120 mesh) coated with 10% Carbowax 20M (Union Carbide Corp., New York, N.Y., U.S.A.). The column temperature was 850 for the analysis of isopentenol and dimethylallyl alcohol (cf.…”

mentioning

confidence: 99%

Isopentenyl pyrophosphate isomerase from liver

Holloway

Popják

1968

Biochemical Journal

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Isopentenyl pyrophosphate isomerase (EC 5.3.3.2) was purified from extracts of pig liver by ammonium sulphate fractionation and by gel filtration. After about 20-fold purification the preparations were free of phosphatase and prenyltransferase (EC 2.5.1.1), the two enzymes that could have interfered with the assays. The isomerase has a distinct pH optimum at 6.0 and is activated by Mn(2+) in preference to Mg(2+). The K(m) value for isopentenyl pyrophosphate is 4x10(-6)m. The equilibrium of the reaction favours the formation of dimethylallyl pyrophosphate. The reversibility of the isomerase reaction was demonstrated directly by the formation of isopentenyl pyrophosphate from dimethylallyl pyrophosphate. It is suggested that two prenyl isomerases might exist, one involved in the synthesis of trans- and another in the synthesis of cis-polyprenyl substances.

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Simultaneous Measurement of C14 and H3 during Gas-Liquid Chromatography

Cited by 5 publications

References 9 publications

Studies on the biosynthesis of cholesterol. XVII. The asymmetric synthesis of a symmetrical molecule

Studies on the biosynthesis of cholesterol. XVII. The asymmetric synthesis of a symmetrical molecule

Synthesis of 10,11-dihydrofarnesyl pyrophosphate from 6,7-dihydrogeranyl pyrophosphate by prenyltransferase

Isopentenyl pyrophosphate isomerase from liver

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