Simultaneous Measurement of Dissolved Oxygen and Oxidation-Reduction Potentials in the Aerobic Culture

The redox potential: Its use and control in biotechnology

“…Wimpenny's investigations have been mentioned already. Shibai et al (1974) investigated the influence of redox potential upon the microbial production of inosine. In a previous paper (Ishizaki eta/., 1974) the same authors had demonstrated that the redox potential, measured with a platinum electrode, depended on pH value, dissolved oxygen concentration, equilibrium constant, and oxidation reduction potentials in the liquid.…”

An electromic controller for maintaining low dissolved oxygen levels in a bench-top fermentor

“…Oxygen can be regarded as a nutrient as well as a regulator of microbial metabolism (Mukhopadhyay and Ghose, 1976). For example, low DOT levels have been reported to influence the formation of intracytoplasmic membranes (Post et al, 1979), the production and excretion of various metabolites (Akashi et al, 1979;Barnes et al, 1978;Calleja et al, 1986;Feren and Squires, 1969;Shibai et al, 1974), and in general can alter the metabolic pathways of facultative anaerobes (Bergersen et al, 1982;Heinemann et al, 1970;Mukhopadhyay and Ghose, 1976;Poole and Chance, 1981;Thomas et al, 1972). Even in the ethanol fermentation with Saccharomyces cerevisiae, traditionally regarded as an anaerobic process, DOT levels of about 0.05% air saturation and higher minimized loss of cell viability (Cysewski and Wilke, 1976;Nagodawithana et al, 1974).…”

Relationship between culture redox potential and culture fluorescence inCorynebacterium glutamicum

“…In microaerobic and anaerobic systems, under conditions of constant pH and temperature, the logarithm of the dissolved oxygen (DO) concentration can be linearly related to the CRP. Culture redox potential measurements are also far more sensitive to trace oxygen levels than DO measurements (Shibai et al, 1974). It has been shown that CRP can be controlled by manipulation of the dissolved oxygen tension and this technique was successfully used in the optimization of amino acid production by C. glutamicum .…”