Linear birefringence is a fundamental property of optically anisotropic
media, defined by the difference in refractive index experienced by
light polarized along orthogonal directions. It is usually manifested
in microscopically aligned molecular systems, where a preferential
direction of light–matter interaction is created. For instance, the
anisotropic structure of calcite crystal causes the famous
double-refraction phenomenon. Another common example is commercial
adhesive tapes, which are polymeric materials possessing birefringent
properties due to their manufacturing processes. The intrinsic
relation between birefringence and molecular alignment forges a new
analytical route to study materials such as polymeric thin films.
Therefore, the capacity of measuring linear birefringence and its fast
axis is of paramount importance for the science of anisotropic
molecular systems. In this contribution, a comprehensive approach to
acquire linear birefringence using rotating-sample transmission Stokes
spectropolarimetry is presented and applied to transparent adhesive
tapes as a case study. The experimental setup comprises a thermal
light source and a spectropolarimeter capable of determining
wavelength distributions of Stokes parameters. The samples are
carefully aligned in a rotating mount and subjected to a fixed
broadband vertically polarized light beam. Then, the transmitted light
is analyzed using a rotating retarder type of spectropolarimeter.
Through systematic variation of the sample’s angular position, the
Stokes parameters of transmitted light are measured for each
transmitted wavelength as a function of the sample’s angular position.
The linear retardance and fast axis direction relative to the tape’s
long axis are then determined from the modulation of Stokes parameters
over sample rotation. The model derivation, experimental procedure,
and signal processing protocol are described in detail, and the
approach is verified with a simple correlation between linear
retardance and the number of stacked layers of tape.