Simultaneous Measurement of Plasma Catecholamine (Norepinephrine, Epinephrine, and Dopamine) and Free N—Methyl Dopamine (Epinine) Levels, by HPLC with Electrochemical Detection

Musso, Natale R.; Vergassola, C.; Pende, Aldo; Lotti, G

doi:10.1080/01483919008049025

Cited by 29 publications

(6 citation statements)

References 5 publications

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“…Many reports are mentioned in the literature for the determination of epinine or dobutamine using various analytical methods, including liquid chromatography, normal Raman spectroscopy, spectrophotometry, spectrofluorimetry, capillary electrophoresis, and chemiluminescence [16][17][18][19][20][21][22][23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of GO/ZIF-67 Nanocomposite: Investigation of Catalytic Activity for the Determination of Epinine in the Presence of Dobutamine

et al. 2022

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In this study, we prepared graphene oxide (GO)/ZIF-67 nanocomposites. Therefore, GO/ZIF-67 nanocomposites were used as a modifier on a screen-printed electrode (GO/ZIF-67/SPE) for studying the electrochemical behavior of epinine in phosphate buffer saline (PBS) at pH 7.0 with voltammetry techniques. The GO/ZIF-67/SPE showed greater electrocatalytic activities than the bare SPE. As a result, the GO/ZIF-67/SPE was utilized for additional electrochemical examinations. The epinine concentration determination was in the range 9.0 × 10−8 M to 5.0 × 10−4 M, and the limit of detection (LOD) as well as the limit of quantification (LOQ) equaled 2.0 and 6.6 nM, respectively. From the scan rate study, the oxidation of epinine was found to be diffusion-controlled, and the simultaneous detection of epinine and dobutamine were well achieved with the differential pulse voltammetric (DPV) technique. Moreover, the stability and reproducibility of epinine at the GO/ZIF-67/SPE was studied, and the use of the GO/ZIF-67/SPE to detect epinine and dobutamine in real samples was furthermore successfully demonstrated.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of GO/ZIF-67 Nanocomposite: Investigation of Catalytic Activity for the Determination of Epinine in the Presence of Dobutamine

et al. 2022

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“…1,2 Catecholamines are also related to the sympathetic medullar system, which is particularly sensitive to mental and physical stress. 3,4 Up to now, high-performance liquid chromatography (HPLC) with electrochemical [5][6][7] or optical 8 detections has been frequently used to detect catecholamines. Although catecholamines can be selectively detected from ascorbic acid and uric acid by the HPLC technique, this method requires a pretreatment process, and the detection measurements take time.…”

Section: Introductionmentioning

confidence: 99%

Detection of Adrenaline on Poly(3-aminobenzylamine) Ultrathin Film by Electrochemical-Surface Plasmon Resonance Spectroscopy

et al. 2010

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In this Article, we present a novel method to detect adrenaline on poly(3-aminobenzylamine) (PABA) ultrathin films by electrochemical-surface plasmon resonance (EC-SPR) spectroscopy. We prepared a PABA film, which specifically reacts with adrenaline, on a gold electrode by electropolymerization of 3-aminobenzylamine. The specific reaction of benzylamine within the PABA structure with adrenaline was studied by XPS, UV-vis spectroscopy, and EC-SPR techniques. Adrenaline was detected in real time by EC-SPR spectroscopy, which provides simultaneous monitoring of both optical SPR reflectivity and electrochemical current responses upon injecting adrenaline into the PABA thin film. The number of changes in both current and SPR reflectivity on the injection of adrenaline exhibited the linear relation to the concentration, and the detection limit was 100 pM. The responses were distinctive to those for uric acid and ascorbic acid, which are major interferences of adrenaline.

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“…Catecholamines in plasma from cows and sheep were determined using high‐performance liquid chromatography (HPLC) with electrochemical detection (Kågedal & Goldstein, 1988; Musso et al , 1990). Plasma samples from the calves were not assayed for catecholamines.…”

Section: Methodsmentioning

confidence: 99%

The effects of medetomidine and its reversal with atipamezole on plasma glucose, cortisol and noradrenaline in cattle and sheep

Ranheim

Horsberg

Søli

et al. 2000

Vet Pharm & Therapeutics

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The effects of medetomidine and its reversal with atipamezole on plasma glucose, cortisol and noradrenaline in cattle and sheep. J. vet. Pharmacol. Therap. 23,[379][380][381][382][383][384][385][386][387] In the present study, we report the effect of medetomidine followed by atipamezole on plasma glucose, cortisol and noradrenaline in calves, cows and sheep. Eight calves, eight lactating dairy cows and eight adult female sheep were included in a crossover trial. The animals were injected i.v. with medetomidine (40 mg/kg), followed 60 min later by atipamezole i.v. (200 mg/ kg) or saline. The wash-out period between experiments was 1 or 2 weeks. In every animal, medetomidine induced a marked hyperglycaemia, which was reversed by atipamezole. Cortisol levels increased significantly in cows and sheep, reaching levels 4-8-fold higher than the baseline levels 25 -45 min after injection of medetomidine. Atipamezole did not affect the cortisol levels, except in sheep where an increase was observed. Plasma levels of noradrenaline decreased in cows and sheep after medetomidine injection, reflecting the inhibition of sympathetic activity by the drug. After injection of the antagonist, there was a large increase in noradrenaline levels. In conclusion, a high dose of medetomidine does not seem to reduce the overall endocrine stress response in cattle and sheep, which has previously been reported in other species.(Paper

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Simultaneous Measurement of Plasma Catecholamine (Norepinephrine, Epinephrine, and Dopamine) and Free N—Methyl Dopamine (Epinine) Levels, by HPLC with Electrochemical Detection

Cited by 29 publications

References 5 publications

Synthesis and Characterization of GO/ZIF-67 Nanocomposite: Investigation of Catalytic Activity for the Determination of Epinine in the Presence of Dobutamine

Synthesis and Characterization of GO/ZIF-67 Nanocomposite: Investigation of Catalytic Activity for the Determination of Epinine in the Presence of Dobutamine

Detection of Adrenaline on Poly(3-aminobenzylamine) Ultrathin Film by Electrochemical-Surface Plasmon Resonance Spectroscopy

The effects of medetomidine and its reversal with atipamezole on plasma glucose, cortisol and noradrenaline in cattle and sheep

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