1997
DOI: 10.1093/clinchem/43.12.2424
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Simultaneous Measurement of Serum Tryptophan and Kynurenine by HPLC,

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Cited by 390 publications
(192 citation statements)
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“…Tryptophan and kynurenine concentrations in the controls (Table 2) compare rather well with published data (Widner et al 1997). In the lung cancer patients tryptophan concentrations were significantly lower than in controls, kynurenine concentrations did not differ between the groups (Table 2).…”
Section: Resultssupporting
confidence: 87%
See 1 more Smart Citation
“…Tryptophan and kynurenine concentrations in the controls (Table 2) compare rather well with published data (Widner et al 1997). In the lung cancer patients tryptophan concentrations were significantly lower than in controls, kynurenine concentrations did not differ between the groups (Table 2).…”
Section: Resultssupporting
confidence: 87%
“…Age ( were stored at -20°C until assayed. Serum tryptophan and kynurenine concentrations were determined by highperformance liquid chromatography as described, the ratio of kynurenine and tryptophan concentrations (kyn/trp) was calculated to estimate the activity of indoleamine (2,3)-dioxygenase (Widner et al 1997). Neopterin concentrations were determined by ELISA (BRAHMS, Berlin, Germany) according to the manufacturer's instructions.…”
Section: Gendermentioning
confidence: 99%
“…We undertook HPLC on reversed phase for tryptophan breakdown measurements, as described previously. (14,15) In brief, serum samples were diluted with potassium phosphate buffer (0.05 mol/L, pH 6.0) containing the internal calibrator 3-nitro-L-tyrosine (100 lmol/L). Protein was then precipitated with 50 lL trichloroacetic acid (2 mol/L) and removed by centrifugation.…”
Section: Methodsmentioning
confidence: 99%
“…Tryptophan and kynurenine concentrations from the cell supernatant were determined by high-performance liquid chromatography, as described previously [23]. After precipitation of protein with trichloroacetic acid, tryptophan was measured by fluorescence detection at 285 nm excitation and 365 nm emission wavelengths.…”
Section: Indoleamine 23-dioxygenase Assaymentioning
confidence: 99%