Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics

Cook, Ellen B.; Stahl, James L.; Lowe, Larry; Chen, R.; Morgan, Edward T.; Wilson, Jerry D.; Varró, R; Chan, Antoni; Graziano, Frank M.; Barney, Neal P.

doi:10.1016/s0022-1759(01)00407-0

Cited by 258 publications

(173 citation statements)

References 18 publications

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“…As a result, the number of assays which can be performed is more limited than in array defined by two fluorochromes (6). However, one advantage of the CBA system is that it can be performed on a clinical flow cytometer already installed in the laboratory.…”

Section: Cytometric Bead Assay (Cba)mentioning

confidence: 99%

Multiplex bead array assays: Performance evaluation and comparison of sensitivity to ELISA☆

Elshal

McCoy

2006

Methods

499

402

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The measurement of soluble cytokines and other analytes in serum and plasma is becoming increasingly important in the study and management of many diseases. As a result, there is a growing demand for rapid, precise, and cost-effective measurement of such analytes in both clinical and research laboratories. Multiplex bead array assays provide quantitative measurement of large numbers of analytes using an automated 96-well plate format. ELISAs (enzyme linked immunosorbent assay) have long been the standard for quantitative analysis of cytokines and other biomarkers, but are not well suited for high throughput multiplex analyses. However, prior to replacement of ELISA assays with multiplex bead array assays, there is a need to know how comparable these two methods are for quantitative analyses. A number of published studies have compared these two methods and it is apparent that certain elements of these assays, such as the clones of monoclonal antibodies used for detection and reporting, are pivotal in obtaining similar results from both assays. By careful consideration of these variables, it should be possible to utilize multiplex bead array assays in lieu of ELISAs for studies requiring high throughput analysis of numerous analytes.

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Section: Cytometric Bead Assay (Cba)mentioning

confidence: 99%

Multiplex bead array assays: Performance evaluation and comparison of sensitivity to ELISA☆

Elshal

McCoy

2006

Methods

499

402

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“…Cytokine concentrations in each culture supernatant were quantified with either BD TM Cytometric Bead Array kit (BD Bioscience, San Diego, CA, USA) (Cook et al 2001) or FlowCytomix TM multiplex kit (Bender MedSystems GmBH, Vienna, Austria). In brief, as for CBA set-up, 50 µL samples or known concentrations of standard samples (0-5000 pg/ml) were added to capture beads conjugated with Ab for each cytokine followed by an addition of anti-cytokine Ab-phycoerythrin (PE) reagent (detection reagent).…”

Section: Quantification Of Cytokinementioning

confidence: 99%

Prevention of experimental autoimmune uveoretinitis by blockade of osteopontin with small interfering RNA

Iwata

Kitamura

Saito

et al. 2010

Experimental Eye Research

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Osteopontin (OPN) is elevated during the progression of experimental autoimmune uveoretinitis (EAU) in C57BL/6 (B6) mice. Furthermore, EAU symptoms are ameliorated in OPN knockout mice or in B6 mice treated with anti-OPN antibody (M5). Recently, OPN has been shown to promote the Th1 response not only in the extracellular space as a secretory protein but also in cytosol as a signaling component. Thus, we attempted to reduce OPN in both compartments by using a small interfering RNA (siRNA) targeting the OPN coding sequence (OPN-siRNA).

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“…TNF-α is also one of a number of cytokines found in tears and its expression is elevated in allergic conjunctivitis (Cook et al, 2001). TNF-α is secreted by corneal epithelial cells in response to an inflammatory stimulation caused by bacterial and viral infections (Minami et al, 2002;Kurpakus-Wheater et al, 2003;Bitko et al, 2004;Kumar et al, 2004).…”

Section: K + Channel Activity Evoked By Tnf-αmentioning

confidence: 99%

Stress-induced corneal epithelial apoptosis mediated by K+ channel activation

2006

Progress in Retinal and Eye Research

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One of the functional roles of the corneal epithelial layer is to protect the cornea, lens and other underlying ocular structures from damages caused by environmental insults. It is important for corneal epithelial cells to maintain this function by undergoing continuous renewal through a dynamic process of wound healing. Previous studies in corneal epithelial cells have provided substantial evidence showing that environmental insults, such as ultraviolet (UV) irradiation and other biohazards, can induce stress-related cellular responses resulting in apoptosis and thus interrupt the dynamic process of wound healing. We found that UV irradiation-induced apoptotic effects in corneal epithelial cells are started by the hyperactivation of K + channels in the cell membrane resulting in a fast loss of intracellular K + ions. Recent studies provide further evidence indicating that these complex responses in corneal epithelial cells are resulted from the activation of stressrelated signaling pathways mediated by K + channel activity. The effect of UV irradiation on corneal epithelial cell fate shares common signaling mechanisms involving the activation of intracellular responses that are often activated by the stimulation of various cytokines. One piece of evidence for making this distinction is that at early times UV irradiation activates a Kv3.4 channel in corneal epithelial cells to elicit activation of c-Jun N-terminal kinase cascades and p53 activation leading to cell cycle arrest and apoptosis. The hypothetic model is that UV-induced potassium channel hyperactivity as an early event initiates fast cell shrinkages due to the loss of intracellular potassium, resulting in the activation of scaffolding protein kinases and cytoskeleton reorganizations. This review article presents important control mechanisms that determine Kv channel activity-mediated cellular responses in corneal epithelial cells, involving activation of stress-induced signaling pathways, arrests of cell cycle progression and/or induction of apoptosis.

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Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs. non-allergics

Cited by 258 publications

References 18 publications

Multiplex bead array assays: Performance evaluation and comparison of sensitivity to ELISA☆

Multiplex bead array assays: Performance evaluation and comparison of sensitivity to ELISA☆

Prevention of experimental autoimmune uveoretinitis by blockade of osteopontin with small interfering RNA

Stress-induced corneal epithelial apoptosis mediated by K+ channel activation

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